Other Characterized Proteins Similar to lysA

Finding the characterized proteins for lysA (diaminopimelate decarboxylase) in L-lysine biosynthesis

Or see clustering for step lysA

Or see all steps for L-lysine biosynthesis

Or cluster curated proteins matching a keyword

Definition of lysA

• Curated proteins or TIGRFams with EC 4.1.1.20
• Ignore hits to A0A0H3H393 when looking for 'other' hits (lysine decarboxylase (EC 4.1.1.18))
• Predicted: UniProt sequence U7D9L6: SubName: Full=Diaminopimelate decarboxylase {ECO:0000313|EMBL:ERP38717.1};
• Ignore hits to MONOMER-21197 when looking for 'other' hits (dimethylsulfonio-2-hydroxybutanoate hydroxylase/decarboxylase)
• Comment: A0A0H3H393 is reported to be a lysine decarboxylase but is 87% identical to E. coli's diaminopimelate decarboxylase, so it is ignored. CALK_RS03395 (U7D9L6) has conserved functional residues (see alignment to 1ko0A) and is in an operon with meso-diaminopimelate D-dehydrogenase (ddh), so it must be the missing lysA. MetaCyc annotates THAPSDRAFT_20613 (link), which is probably a diaminopimelate decarboxylase, as a dimethylsulfonio-2-hydroxybutanoate hydroxylase/decarboxylase, but there is no experimental evidence, so it is ignored.

Fetched 17 sequences

Running ublast to find other characterized proteins with 40% identity and 70% coverage

Found hits to 1 other characterized sequences. (Found 17 hits including self hits.)

Removing ignored items reduces this to 0 close sequences.

Close but ignored sequences

(Sequences that are similar to these will still be high-confidence candidates for lysA.)

A0A0H3H393 lysine decarboxylase (EC 4.1.1.18) from Klebsiella oxytoca
PFams: Orn_DAP_Arg_deC, Orn_Arg_deC_N
420 amino acids: PaperBLAST, CDD, BRENDA::A0A0H3H393
87% identical to

b2838 diaminopimelate decarboxylase (EC 4.1.1.20) from Escherichia coli K-12 substr. MG1655
P00861 diaminopimelate decarboxylase (EC 4.1.1.20) from Escherichia coli (strain K12)
DCDA_ECOLW / E0IWI3 Diaminopimelate decarboxylase; DAP decarboxylase; DAPDC; EC 4.1.1.20 from Escherichia coli (strain ATCC 9637 / CCM 2024 / DSM 1116 / LMG 11080 / NBRC 13500 / NCIMB 8666 / NRRL B-766 / W)
DCDA_ECOLI / P00861 Diaminopimelate decarboxylase; DAP decarboxylase; DAPDC; EC 4.1.1.20 from Escherichia coli (strain K12)
P00861 diaminopimelate decarboxylase (EC 4.1.1.20) from Escherichia coli
PFams: Orn_DAP_Arg_deC, Orn_Arg_deC_N
420 amino acids: PaperBLAST, CDD