Other Characterized Proteins Similar to PPYAT

GapMind for Amino acid biosynthesis

Other Characterized Proteins Similar to PPYAT

Finding the characterized proteins for PPYAT (phenylpyruvate aminotransferase) in L-phenylalanine biosynthesis

Or see clustering for step PPYAT

Or see all steps for L-phenylalanine biosynthesis

Or cluster curated proteins matching a keyword

Definition of PPYAT

Curated proteins or TIGRFams with EC 2.6.1.57
Curated proteins or TIGRFams with EC 2.6.1.1
Curated proteins or TIGRFams with EC 2.6.1.5
Ignore hits to P39643 when looking for 'other' hits (Transaminase BacF; Transaminase A; EC 2.6.1.-. 3-[(2S,5R)-5-hydroxy-7-oxabicyclo[4.1.0]heptan-2-yl]-2-oxopropanoate aminotransferase (EC 2.6.1.57))
Ignore hits to H7CE71 when looking for 'other' hits (aromatic-amino-acid transaminase (EC 2.6.1.57))
Curated sequence PHETRANTHAUERA-MONOMER: aromatic-amino-acid aminotransferase (EC 2.6.1.1)
Curated sequence A0A060PQX5: branched-chain-amino-acid transaminase (EC 2.6.1.42)
Ignore hits to items matching EC 2.6.1.27 when looking for 'other' hits
Ignore hits to items matching 2.6.1.42 when looking for 'other' hits
Ignore hits to P12343 when looking for 'other' hits (Aspartate aminotransferase, cytoplasmic; cAspAT; Cysteine aminotransferase, cytoplasmic; Cysteine transaminase, cytoplasmic; cCAT; Glutamate oxaloacetate transaminase 1; Transaminase A; EC 2.6.1.1; EC 2.6.1.3)
Ignore hits to MONOMER-15918 when looking for 'other' hits (phosphoserine aminotransferase monomer (EC 2.6.1.52; EC 2.6.1.1))
Ignore hits to Q93QX0 when looking for 'other' hits (Bifunctional aspartate aminotransferase and L-aspartate beta-decarboxylase; Aspartate 4-decarboxylase; ASD; AsdA; EC 2.6.1.1; EC 4.1.1.12. aspartate 4-decarboxylase (EC 4.1.1.12))
Comment: These enzymes are usually annotated as aromatic-amino-acid transaminase, but only the activity on phenylpyruvate (to form phenylalanine) is relevant here. Phenylalanine is also considered a substrate for aspartate aminotransferases, P39643 is annotated with this EC number but is involved in bacilysin biosynthesis and probably wouldn't form phenylalanine. H7CE71 is misannotated as this in BRENDA. link has this activity but is annotated with a different EC number. A0A060PQX5 has this activity but is given a different EC number in BRENDA. link and Q93QX0 probably do not have this activity. Many branched-chain or tryptophan transaminases are also active on phenylalanine so any similarity to them is ignored. Ignore the very short protein P12343

Fetched 111 sequences

Running ublast to find other characterized proteins with 40% identity and 70% coverage

Found hits to 49 other characterized sequences. (Found 160 hits including self hits.)

Removing ignored items reduces this to 37 close sequences.

Close sequences

(Sequences that are similar to these will not be high-confidence candidates for PPYAT.)

Q845W8 aspartate 4-decarboxylase (EC 4.1.1.12) from Pseudomonas dacunhae
PFams: Aminotran_1_2
533 amino acids: PaperBLAST, CDD, BRENDA::Q845W8
84% identical to

ASDP_PSESP / Q53IZ1 Bifunctional aspartate aminotransferase and L-aspartate beta-decarboxylase; Aspartate 4-decarboxylase; Asd; AsdP; EC 2.6.1.1; EC 4.1.1.12 from Pseudomonas sp.
Q53IZ1 aspartate 4-decarboxylase (EC 4.1.1.12) from Pseudomonas sp.
PFams: Aminotran_1_2
531 amino acids: PaperBLAST, CDD

O57946 kynurenine-oxoglutarate transaminase (EC 2.6.1.7) from Pyrococcus horikoshii
PFams: Aminotran_1_2, Asp_aminotransf
428 amino acids: PaperBLAST, CDD, BRENDA::O57946
75% identical to

A0A060PQX5 branched-chain-amino-acid transaminase (EC 2.6.1.42) from Thermococcus sp.
PFams: Aminotran_1_2, Asp_aminotransf
417 amino acids: PaperBLAST, CDD

Q9SIV0 alkyl-thiohydroximate C-S lyase (EC 4.4.1.13) from Arabidopsis thaliana
SUR1_ARATH / Q9SIV0 S-alkyl-thiohydroximate lyase SUR1; Protein ABERRANT LATERAL ROOT FORMATION 1; Protein HOOKLESS 3; Protein ROOTY; Protein ROOTY 1; Protein SUPERROOT 1; EC 4.4.1.- from Arabidopsis thaliana (Mouse-ear cress)
PFams: Aminotran_1_2
462 amino acids: PaperBLAST, CDD, SwissProt::Q9SIV0
72% identical to

Q67Y55 tyrosine transaminase (EC 2.6.1.5) from Arabidopsis thaliana
PFams: Aminotran_1_2
449 amino acids: PaperBLAST, CDD

Q7TSV6 aspartate racemase (EC 5.1.1.13) from Mus musculus
PFams: Aminotran_1_2
404 amino acids: PaperBLAST, CDD, BRENDA::Q7TSV6
74% identical to

Q8NHS2 aspartate transaminase (EC 2.6.1.1) from Homo sapiens
PFams: Aminotran_1_2
421 amino acids: PaperBLAST, CDD

Q0IS70 nicotianamine aminotransferase (EC 2.6.1.80) from Oryza sativa
PFams: Aminotran_1_2, Aminotran_1_2
481 amino acids: PaperBLAST, CDD, BRENDA::Q0IS70
59% identical to