Other Characterized Proteins Similar to argX

Finding the characterized proteins for argX (glutamate--LysW ligase) in L-proline biosynthesis

Or see clustering for step argX

Or see all steps for L-proline biosynthesis

Or cluster curated proteins matching a keyword

Definition of argX

• Curated proteins or TIGRFams with EC 6.3.2.60
• Curated sequence Q970U6: Glutamate--LysW ligase ArgX; EC 6.3.2.-
• Ignore hits to items matching 6.3.2.43 when looking for 'other' hits
• Comment: There is also an archaeal pathway from glutamate to ornithine with LysW as the carrier protein, instead of N-acyl intermediates. This pathway is analogous to the conversion of alpha-aminoadipate to lysine, and many of the enzymes are bifunctional. But the initial ligation of LysW to arginine has a dedicated enzyme in many archaea. Q970U6 is given a generic EC number in SwissProt, but has this function. EC 6.4.2.43 (LysW-2-aminoadipate ligases) are ignored because some are bifunctional (PMC5076833).

Fetched 3 sequences

Running ublast to find other characterized proteins with 40% identity and 70% coverage

Found hits to 2 other characterized sequences. (Found 5 hits including self hits.)

Removing ignored items reduces this to 0 close sequences.

Close but ignored sequences

(Sequences that are similar to these will still be high-confidence candidates for argX.)

Q5SH23 L-2-aminoadipate--LysW ligase monomer (EC 6.3.2.43) from Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8)
LYSX_THET8 / Q5SH23 Alpha-aminoadipate--LysW ligase LysX; AAA--LysW ligase LysX; EC 6.3.2.43 from Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8)
Q5SH23 [amino group carrier protein]-L-2-aminoadipate ligase (EC 6.3.2.43) from Thermus thermophilus
PFams: ATP-grasp_3, CPSase_L_D2, RimK, GSH-S_ATP
280 amino acids: PaperBLAST, CDD, SwissProt::Q5SH23
46% identical to

Q5JFW0 lysyltransferase (EC 2.3.2.3); [amino group carrier protein]-L-2-aminoadipate ligase (EC 6.3.2.43); glutamate---[amino group carrier protein] ligase (EC 6.3.2.60) from Thermococcus kodakarensis
PFams: RimK
273 amino acids: PaperBLAST, CDD

LYSX_SACS2 / Q7SI95 Alpha-aminoadipate--LysW ligase LysX; AAA--LysW ligase LysX; EC 6.3.2.43 from Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2) (Sulfolobus solfataricus)
PFams: RimK, CPSase_L_D2, GSH-S_ATP
275 amino acids: PaperBLAST, CDD, SwissProt::Q7SI95
40% identical to

Q5JFW0 lysyltransferase (EC 2.3.2.3); [amino group carrier protein]-L-2-aminoadipate ligase (EC 6.3.2.43); glutamate---[amino group carrier protein] ligase (EC 6.3.2.60) from Thermococcus kodakarensis
PFams: RimK
273 amino acids: PaperBLAST, CDD