Other Characterized Proteins Similar to trpA

GapMind for Amino acid biosynthesis

Other Characterized Proteins Similar to trpA

Finding the characterized proteins for trpA (indoleglycerol phosphate aldolase) in L-tryptophan biosynthesis

Or see clustering for step trpA

Or see all steps for L-tryptophan biosynthesis

Or cluster curated proteins matching a keyword

Definition of trpA

HMM TIGR00262
Curated sequence Q9YGA9: tryptophan synthase subunit α (EC 4.1.2.8). tryptophan synthase (subunit 1/2) (EC 4.2.1.20)
UniProt sequence A0A3N7DTH5: RecName: Full=Tryptophan synthase alpha chain {ECO:0000256|HAMAP-Rule:MF_00131}; EC=4.2.1.20 {ECO:0000256|HAMAP-Rule:MF_00131};
UniProt sequence H0BV16: RecName: Full=Tryptophan synthase alpha chain {ECO:0000256|HAMAP-Rule:MF_00131}; EC=4.2.1.20 {ECO:0000256|HAMAP-Rule:MF_00131};
UniProt sequence M4NLA4: RecName: Full=Tryptophan synthase alpha chain {ECO:0000256|HAMAP-Rule:MF_00131}; EC=4.2.1.20 {ECO:0000256|HAMAP-Rule:MF_00131};
Ignore hits to items matching EC 4.2.1.20 when looking for 'other' hits
Ignore hits to items matching EC 4.1.2.8 when looking for 'other' hits
Comment: This reaction is 4.1.2.8 by itself or part of 4.2.1.20. The enzyme from Pyrolobus is not matched by the HMM, but is similar to the characterized trpA from Thermococcus (Q9YGA9, see PMID:11029579) APJEIK_06300 from Pedobacter sp. FW305-3-2-15-E-R2A2 (A0A3N7DTH5) can complement a trpA- strain of E. coli (Bradley Biggs). GAMENC_07330 from Acidovorax sp. FHTAMBA (H0BV16) can complement a trpA- strain of E. coli (Bradley Biggs); also it is very similar to Ac3H11_1535, which has auxotrophic phenotypes. LRK54_RS01680 from Rhodanobacter sp. FW104-10B01 (M4NLA4) can complement a trpA- strain of E. coli (Bradley Biggs); also it is similar to N515DRAFT_0116, which has auxotrophic pheonotypes.

Fetched 6 sequences

Running ublast to find other characterized proteins with 40% identity and 70% coverage

Found hits to 4 other characterized sequences. (Found 7 hits including self hits.)

Removing ignored items reduces this to 0 close sequences.

There are also 2 HMM-only high-coverage hits.

HMM-only sequences

(Since these sequences' annotations are outside the definition for trpA, HMM hits that are over 40% similar to these sequences will be scored as moderate confidence.)

CH_122413 bifunctional tryptophan synthase TRPB from Emericella nidulans
PFams: Trp_syntA, PALP
723 amino acids: PaperBLAST, CDD

CH_123254 likely tryptophan synthetase alpha chain from Candida albicans
PFams: Trp_syntA, PALP
702 amino acids: PaperBLAST, CDD

Close but ignored sequences

(Sequences that are similar to these will still be high-confidence candidates for trpA.)

TRP_YEAST / P00931 Tryptophan synthase; EC 4.2.1.20 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
PFams: Trp_syntA, PALP
707 amino acids: PaperBLAST, CDD, SwissProt::P00931
75% identical to

CH_123254 likely tryptophan synthetase alpha chain from Candida albicans
PFams: Trp_syntA, PALP
702 amino acids: PaperBLAST, CDD

Q8U094 tryptophan synthase (subunit 1/2) (EC 4.2.1.20) from Pyrococcus furiosus
PFams: Trp_syntA, His_biosynth
Heteromeric, 248 amino acids: PaperBLAST, CDD, BRENDA::Q8U094
69% identical to

Q9YGA9 tryptophan synthase subunit α (EC 4.1.2.8) from Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1)
Q9YGA9 tryptophan synthase (subunit 1/2) (EC 4.2.1.20) from Thermococcus kodakarensis
PFams: Trp_syntA, Ribul_P_3_epim, His_biosynth
Heteromeric, 251 amino acids: PaperBLAST, CDD

A0A2U8JPY3 tryptophan synthase (indole-salvaging) (EC 4.2.1.122); tryptophan synthase (EC 4.2.1.20) from Psilocybe cubensis
PFams: Trp_syntA, PALP
700 amino acids: PaperBLAST, CDD, BRENDA::A0A2U8JPY3
59% identical to

CH_123254 likely tryptophan synthetase alpha chain from Candida albicans
PFams: Trp_syntA, PALP
702 amino acids: PaperBLAST, CDD

P50908 α subunit of tryptophan synthase (EC 4.1.2.8) from Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8)
PFams: Trp_syntA
239 amino acids: PaperBLAST, CDD, metacyc::MONOMER-344
40% identical to