Other Characterized Proteins Similar to trpB

Finding the characterized proteins for trpB (tryptophan synthase) in L-tryptophan biosynthesis

Or see clustering for step trpB

Or see all steps for L-tryptophan biosynthesis

Or cluster curated proteins matching a keyword

Definition of trpB

• HMM TIGR00263
• UniProt sequence P50383: RecName: Full=Tryptophan synthase beta chain 1; EC=4.2.1.20;
• Curated sequence Q97TX6: tryptophan synthase (EC 4.2.1.20)
• Ignore hits to items matching EC 4.2.1.20 when looking for 'other' hits
• Ignore hits to items matching EC 4.2.1.122 when looking for 'other' hits
• Comment: Some members of TIGR01415 might also have this activity. The family also includes indole-scavenging "trpB-2" proteins, whose presence generally indicates tryptophan synthesis (EC 4.2.1.122). The enzyme from Pyrolobus is not matched by the HMM, but is similar to the characterized trpB from Sulfolobus (P50383, see PMID:11029579) or Saccharolobus (Q97TX6) "TrpB2" proteins are reported to use phosphoserine (an intermediate in serine synthesis) rather than serine as a substrate (PMID:25184516); GapMind does not try to distinguish them from TrpB.

Fetched 4 sequences

Running ublast to find other characterized proteins with 40% identity and 70% coverage

Found hits to 5 other characterized sequences. (Found 8 hits including self hits.)

Removing ignored items reduces this to 0 close sequences.

There are also 2 HMM-only high-coverage hits.

HMM-only sequences

(Since these sequences' annotations are outside the definition for trpB, HMM hits that are over 40% similar to these sequences will be scored as moderate confidence.)

CH_122413 bifunctional tryptophan synthase TRPB from Emericella nidulans
PFams: Trp_syntA, PALP
723 amino acids: PaperBLAST, CDD

CH_123254 likely tryptophan synthetase alpha chain from Candida albicans
PFams: Trp_syntA, PALP
702 amino acids: PaperBLAST, CDD

Close but ignored sequences

(Sequences that are similar to these will still be high-confidence candidates for trpB.)

TRP_YEAST / P00931 Tryptophan synthase; EC 4.2.1.20 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
PFams: Trp_syntA, PALP
707 amino acids: PaperBLAST, CDD, SwissProt::P00931
75% identical to

CH_123254 likely tryptophan synthetase alpha chain from Candida albicans
PFams: Trp_syntA, PALP
702 amino acids: PaperBLAST, CDD

A0A2U8JPY3 tryptophan synthase (indole-salvaging) (EC 4.2.1.122); tryptophan synthase (EC 4.2.1.20) from Psilocybe cubensis
PFams: Trp_syntA, PALP
700 amino acids: PaperBLAST, CDD, BRENDA::A0A2U8JPY3
59% identical to

CH_123254 likely tryptophan synthetase alpha chain from Candida albicans
PFams: Trp_syntA, PALP
702 amino acids: PaperBLAST, CDD

Q5JDJ1 tryptophan synthase (indole-salvaging) (EC 4.2.1.122); tryptophan synthase (subunit 1/2) (EC 4.2.1.20) from Thermococcus kodakarensis
PFams: PALP
Heteromeric, 442 amino acids: PaperBLAST, CDD, BRENDA::Q5JDJ1
55% identical to

P50383 Tryptophan synthase beta chain 1; EC=4.2.1.20
425 amino acids: PaperBLAST, CDD

Q9WZ09 tryptophan synthase β2 subunit (EC 4.2.1.122) from Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8)
PFams: PALP
422 amino acids: PaperBLAST, CDD, metacyc::MONOMER-3543
54% identical to

P50383 Tryptophan synthase beta chain 1; EC=4.2.1.20
425 amino acids: PaperBLAST, CDD

Q9FFW8 tryptophan synthase, beta subunit (EC 4.2.1.122) from Arabidopsis thaliana
Q9FFW8 tryptophan synthase (EC 4.2.1.20) from Arabidopsis thaliana
PFams: PALP
506 amino acids: PaperBLAST, CDD, BRENDA::Q9FFW8
50% identical to

P50383 Tryptophan synthase beta chain 1; EC=4.2.1.20
425 amino acids: PaperBLAST, CDD