Other Characterized Proteins Similar to ilvH
Finding the characterized proteins for ilvH (acetolactate/acetohydroxybutanoate synthase regulatory subunit ) in L-valine biosynthesis
Or see clustering for step ilvH
Or see all steps for L-valine biosynthesis
Or cluster curated proteins matching a keyword
Definition of ilvH
- HMM TIGR00119
- Curated proteins matching acetohydroxy-acid synthase%small
- Curated proteins matching acetohydroxybutanoate synthase, regulatory subunit
- Curated proteins matching small subunit of acetolactate synthase
- Curated sequence P0ADG1: acetolactate synthase (subunit 1/2) (EC 2.2.1.6). acetolactate synthase II subunit IlvM (EC 2.2.1.6)
- Ignore hits to items matching EC 2.2.1.6 when looking for 'other' hits
- UniProt sequence A0A154R0Y7: RecName: Full=Acetolactate synthase small subunit {ECO:0000256|RuleBase:RU368092}; Short=AHAS {ECO:0000256|RuleBase:RU368092}; Short=ALS {ECO:0000256|RuleBase:RU368092}; EC=2.2.1.6 {ECO:0000256|RuleBase:RU368092}; AltName: Full=Acetohydroxy-acid synthase small subunit {ECO:0000256|RuleBase:RU368092};
- Curated sequence Q93YZ7: Acetolactate synthase small subunit 1, chloroplastic; ALS-interacting protein 1; Acetohydroxyacid synthase small subunit 1
- UniProt sequence A0A0H2X4P1: SubName: Full=Acetolactate synthase isozyme II small subunit {ECO:0000313|EMBL:AAY47914.1};
- Predicted: UniProt sequence A0A7J3AYJ4: SubName: Full=Uncharacterized protein {ECO:0000313|EMBL:HGD33185.1};
- Predicted: UniProt sequence A0A161J739: SubName: Full=Acetolactate synthase 3 regulatory subunit domain protein {ECO:0000313|EMBL:ANC53960.1};
- Comment: The isolated catalytic subunit can have some activity on its own, so it's not clear if the regulatory subunit (ilvH) is always required, but ilvH does always seem to be present. P0ADG1 is annotated with this EC number but not explicitly as the small regulatory subunit, so it was added manually. Q93YZ7 is annotated as this but without the EC number, so is added manually. Most regulatory subunits have an N-terminal ACT domain and a C-terminal ACT-like domain, but E. coli IlvM, which is required for the activity of E. coli acetohydroxyacid synthase isoenzyme II, has the N-terminal ACT domain only. We identified several other short (one-domain) regulatory subunits. In Rhodanobacter and related genera, the putative regulatory subunit has just one ACT domain (i.e., LRK54_RS10305, which is nearly identical to A0A154R0Y7). Based on sequence analysis, short ilvH probably maintains the ability to bind valine and to bind the catalytic subunit, but not the ability to bind ATP or other regulatory subunits. Mutant fitness data confirms that LRK54_RS10305 is involved in amino acid biosynthesis. In Xanthomonas campestris, the one ACT-domain protein Xcc-8004.1058.1 (A0A0H2X4P1), which is conserved next to ilvI, has a similar fitness pattern as ilvI (Alice Castaing, unpublished data). Furthermore, its AlphaFold structure is very similar to that of E. coli IlvM (TM-score 0.89, RMSD 1.49 A, foldseek). So Xcc-8004.1058.1 is another short regulatory subunit. Similarly, in Brevundimonas sp. GW460-12-10-14-LB2, the putative ilvH has the ACT domain only (Brev2_1981 = A0A161J739). Many Thermoproteota seem to have a diverged short regulatory subunit, such as the ACT domain protein KCR_RS03285 (A0A7J3AYJ4), which is conserved next to ilvI. Foldseek shows that this protein is similar to the ACT domain of (p)ppGpp synthase but also to IlvH of Staphylococcus aureus, so we predict that it is the regulatory subunit.
Fetched 14 sequences
Running ublast to find other characterized proteins with 40% identity and 70% coverage
Found hits to 3 other characterized sequences. (Found 13 hits including self hits.)
Removing ignored items reduces this to 0 close sequences.
Close but ignored sequences
(Sequences that are similar to these will still be high-confidence candidates for ilvH.)
G4NC97 acetolactate synthase (subunit 2/2) (EC 2.2.1.6) from Pyricularia oryzae
PFams: ACT, ACT_5, ALS_ss_C
Heteromeric, 316 amino acids: PaperBLAST, CDD, BRENDA::G4NC97
70% identical to
CH_122620 small subunit of acetolactate synthase from Emericella nidulans
PFams: ACT, ACT_5, ALS_ss_C
326 amino acids: PaperBLAST, CDD
Q0KCU3 acetohydroxyacid synthase subunit H (EC 2.2.1.6) from Cupriavidus necator (strain ATCC 17699 / DSM 428 / KCTC 22496 / NCIMB 10442 / H16 / Stanier 337)
PFams: ACT, ACT_5, ALS_ss_C
Heteromeric, 163 amino acids: PaperBLAST, CDD, metacyc::MONOMER-18811
59% identical to
b0078 acetolactate synthase / acetohydroxybutanoate synthase, regulatory subunit (EC 2.2.1.6) from Escherichia coli K-12 substr. MG1655
P00894 acetolactate synthase / acetohydroxybutanoate synthase, regulatory subunit (EC 2.2.1.6) from Escherichia coli (strain K12)
P00894 acetolactate synthase (subunit 1/2) (EC 2.2.1.6) from Escherichia coli
GB|BAB96647.2 acetolactate synthase, small subunit; EC 2.2.1.6 from Escherichia coli
PFams: ACT, ACT_5, ALS_ss_C
Heteromeric, 163 amino acids: PaperBLAST, CDD
Q8RL87 acetolactate synthase (subunit 1/2) (EC 2.2.1.6) from Geobacillus stearothermophilus
PFams: ACT_4, ACT, ACT_5, ALS_ss_C
Heteromeric, 169 amino acids: PaperBLAST, CDD, BRENDA::Q8RL87
45% identical to
O08354 acetohydroxy-acid synthase small subunit (EC 2.2.1.6) from Methanococcus aeolicus
PFams: ACT, ACT_5, ALS_ss_C
Heteromeric, 169 amino acids: PaperBLAST, CDD