Other Characterized Proteins Similar to mcl

Finding the characterized proteins for mcl ((S)-citramalyl-CoA pyruvate-lyase) in L-glutamate catabolism

Or see clustering for step mcl

Or see all steps for L-glutamate catabolism

Or cluster curated proteins matching a keyword

Definition of mcl

• Curated proteins or TIGRFams with EC 4.1.3.25
• Ignore hits to GFF3000 when looking for 'other' hits (Malyl-CoA lyase (EC 4.1.3.24))
• Ignore hits to B6E2X2 when looking for 'other' hits (L-malyl-CoA/beta-methylmalyl-CoA lyase; (3S)-malyl-CoA/beta-methylmalyl-CoA lyase; EC 4.1.3.24. malyl-CoA lyase subunit (EC 4.1.3.24))
• Comment: Although the citramalate lyase is described as a single reaction in MetaCyc, the enzyme database entry (EC 4.1.3.22) suggests that it is a two-step reaction: citramalate CoA-transferase (EC 2.8.3.11) and citramalyl-CoA lyase (EC 4.1.3.25). No citramalate CoA-transferase proteins have been linked to sequence, but citramalyl-CoA lyases are known, so only this step is included. Some (S-citramalyl-CoA lyases are very similar to malyl-CoA lyases, which are marked ignore.

Fetched 6 sequences

Running ublast to find other characterized proteins with 40% identity and 70% coverage

Found hits to 4 other characterized sequences. (Found 10 hits including self hits.)

Removing ignored items reduces this to 2 close sequences.

Close sequences

(Sequences that are similar to these will not be high-confidence candidates for mcl.)

P71503 malyl coenzyme A lyase subunit (EC 4.1.3.24) from Methylorubrum extorquens
C5B113 malyl-CoA lyase (EC 4.1.3.24) from Methylorubrum extorquens
PFams: HpcH_HpaI
324 amino acids: PaperBLAST, CDD, BRENDA::C5B113
57% identical to

MCAL_RHOS4 / Q3J5L6 L-malyl-CoA/beta-methylmalyl-CoA lyase; (3S)-malyl-CoA/beta-methylmalyl-CoA lyase; (S)-citramalyl-CoA lyase; EC 4.1.3.24; EC 4.1.3.25 from Rhodobacter sphaeroides (strain ATCC 17023 / 2.4.1 / NCIB 8253 / DSM 158)
Q3J5L6 malyl-CoA lyase (EC 4.1.3.24) from Rhodobacter sphaeroides
PFams: HpcH_HpaI
318 amino acids: PaperBLAST, CDD

CITEL_MYCTU / P9WPE1 Citrate lyase subunit beta-like protein; EC 4.1.-.- from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
PFams: HpcH_HpaI, C-C_Bond_Lyase
273 amino acids: PaperBLAST, CDD, SwissProt::P9WPE1
42% identical to

Q9I562 (S)-citramalyl-CoA lyase (EC 4.1.3.25) from Pseudomonas aeruginosa
PFams: HpcH_HpaI
275 amino acids: PaperBLAST, CDD

Close but ignored sequences

(Sequences that are similar to these will still be high-confidence candidates for mcl.)

B6E2X2 malyl-CoA lyase subunit (EC 4.1.3.24) from Rhodobacter capsulatus
MCAL_RHOCA / B6E2X2 L-malyl-CoA/beta-methylmalyl-CoA lyase; (3S)-malyl-CoA/beta-methylmalyl-CoA lyase; EC 4.1.3.24 from Rhodobacter capsulatus (Rhodopseudomonas capsulata)
PFams: HpcH_HpaI
318 amino acids: PaperBLAST, CDD, SwissProt::B6E2X2
88% identical to

MCAL_RHOS4 / Q3J5L6 L-malyl-CoA/beta-methylmalyl-CoA lyase; (3S)-malyl-CoA/beta-methylmalyl-CoA lyase; (S)-citramalyl-CoA lyase; EC 4.1.3.24; EC 4.1.3.25 from Rhodobacter sphaeroides (strain ATCC 17023 / 2.4.1 / NCIB 8253 / DSM 158)
Q3J5L6 malyl-CoA lyase (EC 4.1.3.24) from Rhodobacter sphaeroides
PFams: HpcH_HpaI
318 amino acids: PaperBLAST, CDD

PGA1_c30490 Malyl-CoA lyase (EC 4.1.3.24) from Phaeobacter inhibens BS107
PFams: HpcH_HpaI
320 amino acids: PaperBLAST, CDD, reanno::Phaeo:GFF3000
87% identical to

MCAL_RHOS4 / Q3J5L6 L-malyl-CoA/beta-methylmalyl-CoA lyase; (3S)-malyl-CoA/beta-methylmalyl-CoA lyase; (S)-citramalyl-CoA lyase; EC 4.1.3.24; EC 4.1.3.25 from Rhodobacter sphaeroides (strain ATCC 17023 / 2.4.1 / NCIB 8253 / DSM 158)
Q3J5L6 malyl-CoA lyase (EC 4.1.3.24) from Rhodobacter sphaeroides
PFams: HpcH_HpaI
318 amino acids: PaperBLAST, CDD