Other Characterized Proteins Similar to dhaK

Finding the characterized proteins for dhaK (dihydroxyacetone:PEP phosphotransferase, subunit K) in glycerol catabolism

Or see clustering for step dhaK

Or see all steps for glycerol catabolism

Or cluster curated proteins matching a keyword

Definition of dhaK

• Curated sequence P76015: glycerone kinase (EC 2.7.1.29). dihydroxyacetone kinase, DhaK subunit; EC 2.7.-.-. PEP-dependent dihydroxyacetone kinase, dihydroxyacetone-binding subunit DhaK; EC 2.7.1.121. dihydroxyacetone kinase subunit K (EC 2.7.1.121). dihydroxyacetone kinase subunit K
• Curated sequence Q92EU2: PTS-dependent dihydroxyacetone kinase 2, dihydroxyacetone-binding subunit DhaK; EC 2.7.1.121
• Curated sequence Q9CIV8: PTS-dependent dihydroxyacetone kinase, dihydroxyacetone-binding subunit DhaK; EC 2.7.1.121
• Ignore hits to items matching 2.7.1.121 when looking for 'other' hits
• Ignore hits to A0A1D3TV19 when looking for 'other' hits (glycerone kinase (EC 2.7.1.29))
• Comment: most prokaryotic dihydroxyacetone kinases are heteromeric and use phosophoenolpyruvate rather than ATP as the phosphoryl donor (EC 2.7.1.121) This is a PTS-like system that relies on EI and Hpr proteins to phosphorylate the dhaM subunit The BRENDA entry does not actually seem to be characterized, not clear if it is dhaK or dhaK'

Fetched 3 sequences

Running ublast to find other characterized proteins with 40% identity and 70% coverage

Found hits to 4 other characterized sequences. (Found 7 hits including self hits.)

Removing ignored items reduces this to 3 close sequences.

Close sequences

(Sequences that are similar to these will not be high-confidence candidates for dhaK.)

DHAQ_LACLA / Q9CIW0 DhaKLM operon coactivator DhaQ; DhaS coactivator DhaQ from Lactococcus lactis subsp. lactis (strain IL1403) (Streptococcus lactis)
PFams: Dak1
328 amino acids: PaperBLAST, CDD, SwissProt::Q9CIW0
43% identical to

DHAK_LACLA / Q9CIV8 PTS-dependent dihydroxyacetone kinase, dihydroxyacetone-binding subunit DhaK; EC 2.7.1.121 from Lactococcus lactis subsp. lactis (strain IL1403) (Streptococcus lactis)
PFams: Dak1
332 amino acids: PaperBLAST, CDD

LERK_PECAS / Q6D8V6 L-erythrulose kinase; EC 2.7.1.209 from Pectobacterium atrosepticum (strain SCRI 1043 / ATCC BAA-672) (Erwinia carotovora subsp. atroseptica)
PFams: Dak1, Dak2
590 amino acids: PaperBLAST, CDD, SwissProt::Q6D8V6
41% identical to

DHAK2_LISIN / Q92EU2 PTS-dependent dihydroxyacetone kinase 2, dihydroxyacetone-binding subunit DhaK; EC 2.7.1.121 from Listeria innocua serovar 6a (strain ATCC BAA-680 / CLIP 11262)
PFams: Dak1
331 amino acids: PaperBLAST, CDD

TKFC_HUMAN / Q3LXA3 Triokinase/FMN cyclase; Bifunctional ATP-dependent dihydroxyacetone kinase/FAD-AMP lyase (cyclizing); EC 2.7.1.28; EC 2.7.1.29; EC 4.6.1.15 from Homo sapiens (Human)
Q3LXA3 triokinase (EC 2.7.1.28); glycerone kinase (EC 2.7.1.29); FAD-AMP lyase (cyclizing) (EC 4.6.1.15) from Homo sapiens
PFams: Dak1, Dak2
575 amino acids: PaperBLAST, CDD, BRENDA::Q3LXA3
41% identical to

b1200 dihydroxyacetone kinase subunit K (EC 2.7.1.121) from Escherichia coli K-12 substr. MG1655
DHAK_ECOLI / P76015 PEP-dependent dihydroxyacetone kinase, dihydroxyacetone-binding subunit DhaK; EC 2.7.1.121 from Escherichia coli (strain K12)
P76015 glycerone kinase (EC 2.7.1.29) from Escherichia coli
P76015 dihydroxyacetone kinase subunit K from Escherichia coli (strain K12)
CH_088626 dihydroxyacetone kinase, DhaK subunit; EC 2.7.-.- from Escherichia coli K12
PFams: Dak1
356 amino acids: PaperBLAST, CDD

Close but ignored sequences

(Sequences that are similar to these will still be high-confidence candidates for dhaK.)

A0A1D3TV19 glycerone kinase (EC 2.7.1.29) from Anaerobium acetethylicum
PFams: Dak1, Dak2
583 amino acids: PaperBLAST, CDD, BRENDA::A0A1D3TV19
50% identical to

DHAK_LACLA / Q9CIV8 PTS-dependent dihydroxyacetone kinase, dihydroxyacetone-binding subunit DhaK; EC 2.7.1.121 from Lactococcus lactis subsp. lactis (strain IL1403) (Streptococcus lactis)
PFams: Dak1
332 amino acids: PaperBLAST, CDD