Other Characterized Proteins Similar to ech

GapMind for catabolism of small carbon sources

Other Characterized Proteins Similar to ech

Finding the characterized proteins for ech (2-methyl-3-hydroxybutyryl-CoA hydro-lyase) in L-isoleucine catabolism

Or see clustering for step ech

Or see all steps for L-isoleucine catabolism

Or cluster curated proteins matching a keyword

Definition of ech

Curated proteins or TIGRFams with EC 4.2.1.17
Curated sequence GFF2389: Enoyl-CoA hydratase [valine degradation] (EC 4.2.1.17)
Ignore hits to items matching 4.2.1.150 when looking for 'other' hits
Ignore hits to Q9SE41 when looking for 'other' hits (enoyl-CoA hydratase (EC 4.2.1.17))
Ignore hits to MONOMER-11695 when looking for 'other' hits (enoyl-CoA hydratase subunit (EC 4.2.1.17))
Ignore hits to PP_3358 when looking for 'other' hits (hydroxycinnamoyl-CoA hydratase-lyase (EC 4.2.1.17; EC 4.1.2.-))
Comment: Psest_2437 (GFF2389) is the enoyl-CoA hydrotase for both isoleucine and valine degradation. A paper claiming that Q9SE41 (PMID:23809632) is ech relies on homology modeling, and it could well be a hydrolase instead; it is 69% identical to the hydrolase from Arabidopsis (Q9LKJ1). And MetaCyc linked Q51969 (MONOMER-11695) from P. putida to ech, but the sequence shown in the paper (PMID:12115060, Fig. 3) is different. And PP_3358 (hydroxycinnamoyl-CoA hydratase-lyase) was incorrectly given this EC number.

Fetched 36 sequences

Running ublast to find other characterized proteins with 40% identity and 70% coverage

Found hits to 20 other characterized sequences. (Found 56 hits including self hits.)

Removing ignored items reduces this to 16 close sequences.

There are also 1 HMM-only high-coverage hits.

Close sequences

(Sequences that are similar to these will not be high-confidence candidates for ech.)

O07453 BadK from Rhodopseudomonas palustris
PFams: ECH_1, ECH_2
258 amino acids: PaperBLAST, CDD, metacyc::MONOMER-943
64% identical to

BPHYT_RS17335 trans-2,3-dehydroadipyl-CoA hydratase (EC 4.2.1.17) from Burkholderia phytofirmans PsJN
PFams: ECH_1, ECH_2
258 amino acids: PaperBLAST, CDD

C8YX87 AcuK from Halomonas sp. HTNK1
PFams: ECH_1, ECH_2
253 amino acids: PaperBLAST, CDD, metacyc::MONOMER-15200
60% identical to

BPHYT_RS17335 trans-2,3-dehydroadipyl-CoA hydratase (EC 4.2.1.17) from Burkholderia phytofirmans PsJN
PFams: ECH_1, ECH_2
258 amino acids: PaperBLAST, CDD

CH_121974 mitochondrial enoyl-CoA hydratase from Emericella nidulans
PFams: ECH_1, ECH_2
289 amino acids: PaperBLAST, CDD, CharProtDB::CH_121974
52% identical to

BPHYT_RS17335 trans-2,3-dehydroadipyl-CoA hydratase (EC 4.2.1.17) from Burkholderia phytofirmans PsJN
PFams: ECH_1, ECH_2
258 amino acids: PaperBLAST, CDD

A4YI89 3-hydroxypropionyl-CoA dehydratase (EC 4.2.1.116) from Metallosphaera sedula (strain ATCC 51363 / DSM 5348 / JCM 9185 / NBRC 15509 / TH2)
HPCD_METS5 / A4YI89 3-hydroxypropionyl-coenzyme A dehydratase; 3-hydroxypropionyl-CoA dehydratase; EC 4.2.1.116 from Metallosphaera sedula (strain ATCC 51363 / DSM 5348 / JCM 9185 / NBRC 15509 / TH2)
A4YI89 3-hydroxypropionyl-CoA dehydratase (EC 4.2.1.116) from Metallosphaera sedula
PFams: ECH_1, ECH_2
259 amino acids: PaperBLAST, CDD, BRENDA::A4YI89
46% identical to

Psest_2437 Enoyl-CoA hydratase [valine degradation] (EC 4.2.1.17) from Pseudomonas stutzeri RCH2
PFams: ECH_1, ECH_2
257 amino acids: PaperBLAST, CDD

Q5SLK3 2-(1,2-epoxy-1,2-dihydrophenyl)acetyl-CoA isomerase (EC 5.3.3.18) from Thermus thermophilus
PFams: ECH_1, ECH_2
254 amino acids: PaperBLAST, CDD, BRENDA::Q5SLK3
45% identical to

PAAG_ECOLI / P77467 1,2-epoxyphenylacetyl-CoA isomerase; EC 5.3.3.18 from Escherichia coli (strain K12)
P77467 2-(1,2-epoxy-1,2-dihydrophenyl)acetyl-CoA isomerase (EC 5.3.3.18) from Escherichia coli
PFams: ECH_1, ECH_2
262 amino acids: PaperBLAST, CDD

b0036 crotonobetainyl-CoA hydratase (EC 4.2.1.149) from Escherichia coli K-12 substr. MG1655
P31551 crotonobetainyl-CoA hydratase (EC 4.2.1.149) from Escherichia coli (strain K12)
CAID_ECOLI / P31551 Carnitinyl-CoA dehydratase; Crotonobetainyl-CoA hydratase; EC 4.2.1.149 from Escherichia coli (strain K12)
P31551 crotonobetainyl-CoA hydratase (EC 4.2.1.149) from Escherichia coli
CH_000803 carnitinyl-CoA dehydratase; EC 4.2.1.- from Escherichia coli K12
PFams: ECH_1, ECH_2
261 amino acids: PaperBLAST, CDD, BRENDA::P31551
43% identical to

DPGD_AMYOR / G4V4T7 Enoyl-CoA-hydratase; EC 4.2.1.17 from Amycolatopsis orientalis (Nocardia orientalis)
PFams: ECH_1, ECH_2
265 amino acids: PaperBLAST, CDD

Q6N3H7 6-carboxyhex-2-enoyl-CoA hydratase from Rhodopseudomonas palustris (strain ATCC BAA-98 / CGA009)
PFams: ECH_1, ECH_2, 3HCDH_N, 3HCDH, 3HCDH
699 amino acids: PaperBLAST, CDD, metacyc::MONOMER-20678
43% identical to

Q08426 peroxisomal bifunctional enzyme (EC 4.2.1.17; EC 1.1.1.35) from Homo sapiens
ECHP_HUMAN / Q08426 Peroxisomal bifunctional enzyme; PBE; PBFE; EC 4.2.1.17; EC 5.3.3.8; EC 1.1.1.35 from Homo sapiens (Human)
PFams: ECH_1, ECH_2, 3HCDH_N, 3HCDH, 3HCDH
723 amino acids: PaperBLAST, CDD

AUHM_MOUSE / Q9JLZ3 Methylglutaconyl-CoA hydratase, mitochondrial; AU-specific RNA-binding enoyl-CoA hydratase; AU-binding enoyl-CoA hydratase; muAUH; Itaconyl-CoA hydratase; EC 4.2.1.18; EC 4.2.1.56 from Mus musculus (Mouse)
PFams: ECH_1, ECH_2
314 amino acids: PaperBLAST, CDD, SwissProt::Q9JLZ3
45% identical to

FER4_USTMA / Q4PEN0 Enoyl-CoA isomerase/hydratase fer4; Fe-regulated protein 4; Ferrichrome A biosynthesis protein fer4; EC 4.2.1.17 from Ustilago maydis (strain 521 / FGSC 9021) (Corn smut fungus)
PFams: ECH_1, ECH_2
274 amino acids: PaperBLAST, CDD

D3RXI4 3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35); 3-hydroxybutyryl-CoA dehydratase (EC 4.2.1.55) from Ferroglobus placidus
PFams: 3HCDH_N, NAD_Gly3P_dh_N, NAD_binding_2, F420_oxidored, 3HCDH, 3HCDH, ECH_1, ECH_2
658 amino acids: PaperBLAST, CDD, BRENDA::D3RXI4
44% identical to

FADB_BACSU / P94549 Probable enoyl-CoA hydratase; EC 4.2.1.17 from Bacillus subtilis (strain 168)
PFams: ECH_1, ECH_2
258 amino acids: PaperBLAST, CDD

Q13825 methylglutaconyl-CoA hydratase subunit (EC 4.2.1.18) from Homo sapiens
AUHM_HUMAN / Q13825 Methylglutaconyl-CoA hydratase, mitochondrial; AU-specific RNA-binding enoyl-CoA hydratase; AU-binding protein/enoyl-CoA hydratase; Itaconyl-CoA hydratase; EC 4.2.1.18; EC 4.2.1.56 from Homo sapiens (Human)
Q13825 methylglutaconyl-CoA hydratase (EC 4.2.1.18) from Homo sapiens
PFams: ECH_1, ECH_2
339 amino acids: PaperBLAST, CDD, BRENDA::Q13825
44% identical to

F4JML5 methylglutaconyl-CoA hydratase (EC 4.2.1.18) from Arabidopsis thaliana
PFams: ECH_1, ECH_2
301 amino acids: PaperBLAST, CDD, BRENDA::F4JML5
44% identical to

Q8GB17 crotonobetainyl-CoA hydratase (EC 4.2.1.149) from Proteus sp.
PFams: ECH_1, ECH_2
261 amino acids: PaperBLAST, CDD, BRENDA::Q8GB17
41% identical to

DPGD_AMYOR / G4V4T7 Enoyl-CoA-hydratase; EC 4.2.1.17 from Amycolatopsis orientalis (Nocardia orientalis)
PFams: ECH_1, ECH_2
265 amino acids: PaperBLAST, CDD

Q1D5Y4 methylglutaconyl-CoA hydratase (EC 4.2.1.18) from Myxococcus xanthus
PFams: ECH_2, ECH_1
258 amino acids: PaperBLAST, CDD, BRENDA::Q1D5Y4
43% identical to

Q9KJE7 putative phenylitaconyl-CoA hydratase subunit from Thauera aromatica
PFams: ECH_1, ECH_2
256 amino acids: PaperBLAST, CDD, metacyc::MONOMER-688
40% identical to

DPGD_AMYOR / G4V4T7 Enoyl-CoA-hydratase; EC 4.2.1.17 from Amycolatopsis orientalis (Nocardia orientalis)
PFams: ECH_1, ECH_2
265 amino acids: PaperBLAST, CDD

BPHYT_RS13545 3-hydroxyadipyl-CoA dehydrogenase (EC 1.1.1.35) from Burkholderia phytofirmans PsJN
PFams: ECH_1, ECH_2, 3HCDH_N, 3HCDH
706 amino acids: PaperBLAST, CDD, reanno::BFirm:BPHYT_RS13545
40% identical to

A9WEU4 propionyl-CoA synthase subunit (EC 1.3.1.84; EC 4.2.1.116; EC 6.2.1.36) from Chloroflexus aurantiacus (strain ATCC 29366 / DSM 635 / J-10-fl)
PFams: ACAS_N, AMP-binding, AMP-binding_C, ECH_1, ECH_2
1822 amino acids: PaperBLAST, CDD, metacyc::MONOMER-13612
42% identical to

FADB_BACSU / P94549 Probable enoyl-CoA hydratase; EC 4.2.1.17 from Bacillus subtilis (strain 168)
PFams: ECH_1, ECH_2
258 amino acids: PaperBLAST, CDD

HMM-only sequences

(Since these sequences' annotations are outside the definition for ech, HMM hits that are over 40% similar to these sequences will be scored as moderate confidence.)

Close but ignored sequences

(Sequences that are similar to these will still be high-confidence candidates for ech.)

A5N5C7 crotonase (EC 4.2.1.150) from Clostridium kluyveri (strain ATCC 8527 / DSM 555 / NCIMB 10680)
PFams: ECH_1, ECH_2
259 amino acids: PaperBLAST, CDD, metacyc::MONOMER-13469
46% identical to

BPHYT_RS17335 trans-2,3-dehydroadipyl-CoA hydratase (EC 4.2.1.17) from Burkholderia phytofirmans PsJN
PFams: ECH_1, ECH_2
258 amino acids: PaperBLAST, CDD

P52046 crotonase monomer (EC 4.2.1.150) from Clostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787)
CRT_CLOAB / P52046 Short-chain-enoyl-CoA hydratase; 3-hydroxybutyryl-CoA dehydratase; Crotonase; EC 4.2.1.150 from Clostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787)
CH_091794 3-hydroxybutyryl-CoA dehydratase; EC 4.2.1.55 from Clostridium acetobutylicum
PFams: ECH_1, ECH_2
261 amino acids: PaperBLAST, CDD, CharProtDB::CH_091794
46% identical to

Q845K2 2,3-dehydroadipyl-CoA hydratase (EC 4.2.1.17) from Pseudomonas sp. Y2
PFams: ECH_1, ECH_2
257 amino acids: PaperBLAST, CDD

CRCH_SYNWW / Q0AVM1 Crotonyl-CoA hydratase; EC 4.2.1.150 from Syntrophomonas wolfei subsp. wolfei (strain DSM 2245B / Goettingen)
PFams: ECH_1, ECH_2
260 amino acids: PaperBLAST, CDD, SwissProt::Q0AVM1
44% identical to

BPHYT_RS17335 trans-2,3-dehydroadipyl-CoA hydratase (EC 4.2.1.17) from Burkholderia phytofirmans PsJN
PFams: ECH_1, ECH_2
258 amino acids: PaperBLAST, CDD

A4YDS4 crotonyl-CoA hydratase/(S)-3-hydroxybutyryl-CoA dehydrogenase (EC 1.1.1.35; EC 4.2.1.150) from Metallosphaera sedula (strain ATCC 51363 / DSM 5348 / JCM 9185 / NBRC 15509 / TH2)
A4YDS4 3-hydroxybutyryl-CoA dehydrogenase (EC 1.1.1.157); 3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35); short-chain-enoyl-CoA hydratase (EC 4.2.1.150) from Metallosphaera sedula
PFams: 3HCDH_N, NAD_binding_2, 3HCDH, 3HCDH, ECH_1, ECH_2
651 amino acids: PaperBLAST, CDD, BRENDA::A4YDS4
42% identical to

Psest_2437 Enoyl-CoA hydratase [valine degradation] (EC 4.2.1.17) from Pseudomonas stutzeri RCH2
PFams: ECH_1, ECH_2
257 amino acids: PaperBLAST, CDD