Other Characterized Proteins Similar to lacA'

GapMind for catabolism of small carbon sources

Finding the characterized proteins for lacA' (periplasmic lactose 3-dehydrogenase, LacA subunit) in lactose catabolism

Or see clustering for step lacA'

Or see all steps for lactose catabolism

Definition of lacA'

Curated sequence CCNA_01706: lactose 3-dehydrogenase flavin subunit (EC 1.1.99.13). lacA: subunit of periplasmic glucoside 3-dehydrogenase (EC 1.1.99.13)
Curated sequence CA265_RS15345: periplasmic glucoside 3-dehydrogenase (lacA subunit) (EC 1.1.99.13)
Ignore hits to items matching 1.1.99.13 when looking for 'other' hits
Comment: LacACB from Caulobacter is the best studied lactose 3-dehydrogenase, but a related system from Pedobacter is also required for lactose utilization. EC 1.1.99.13 includes 3-ketoglycoside dehydrogenases more broadly. Other types of periplasmic 3-ketoglycoside dehydrogenases have been reported (ThuAB from Agrobacterium and Sinorhizobium and BT2158 from Bacteroides thetaiotaomicron) but these do not seem to be involved in lactose utilization. To avoid confusion with galactose catabolism genes, these are named lacA' etc. in GapMind.

Fetched 2 sequences

Running ublast to find other characterized proteins with 40% identity and 70% coverage

Found hits to 2 other characterized sequences. (Found 4 hits including self hits.)

Removing ignored items reduces this to 0 close sequences.

(Sequences that are similar to these will still be high-confidence candidates for lacA'.)

A0A140CUL9 glucoside 3-dehydrogenase (EC 1.1.99.13) from Sphingobacterium faecium
PFams: GMC_oxred_N, GMC_oxred_C
562 amino acids: PaperBLAST, CDD, BRENDA::A0A140CUL9
61% identical to

CA265_RS15345 periplasmic glucoside 3-dehydrogenase (lacA subunit) (EC 1.1.99.13) from Pedobacter sp. GW460-11-11-14-LB5
PFams: GMC_oxred_N, GMC_oxred_C
Heteromeric, 567 amino acids: PaperBLAST, CDD

Echvi_1847 periplasmic glucoside 3-dehydrogenase (lacA subunit) (EC 1.1.99.13) from Echinicola vietnamensis KMM 6221, DSM 17526
PFams: FAD_binding_2, GMC_oxred_N, GMC_oxred_C
Heteromeric, 559 amino acids: PaperBLAST, CDD, reanno::Cola:Echvi_1847
60% identical to