GapMind for catabolism of small carbon sources

 

Clusters of Characterized Proteins

Clustering the characterized proteins for putP (proline:Na+ symporter) in L-proline catabolism

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Definition of putP

Fetched 6 sequences

Running BLASTp

Found similarities, at above 30% identity and 75% coverage, for 6 of these sequences

Found 1 clusters of similar sequences. Download as table or as draft rules or view by organism

Cluster 1 473-512 amino acids (not heteromeric)

The first sequence in each cluster is the seed.

OPUE_BACSU / O06493 Osmoregulated proline transporter OpuE; Osmoprotectant uptake; Proline uptake system from Bacillus subtilis (strain 168)
TC 2.A.21.2.5 / M1TZ79 Proline uptake porter, OpuE (YerK) from Bacillus subtilis subsp. subtilis 6051-HGW
PFams: SSF
492 amino acids: PaperBLAST, CDD, Compare to cluster

b1015 proline:Na+ symporter from Escherichia coli K-12 substr. MG1655
PUTP_ECOLI / P07117 Sodium/proline symporter; Proline permease; Propionate transporter from Escherichia coli (strain K12)
TC 2.A.21.2.1 / P07117 Proline:Na+ symporter, PutP from Escherichia coli
P07117 proline:Na+ symporter from Escherichia coli (strain K12)
PFams: SSF
502 amino acids: PaperBLAST, CDD, Compare to cluster

PUTP_BACSU / P94392 High-affinity proline transporter PutP from Bacillus subtilis (strain 168)
TC 2.A.21.2.4 / J7JIE8 The high affinity nutritional proline uptake porter, PutP from Bacillus subtilis QB928
PFams: SSF
473 amino acids: PaperBLAST, CDD, Compare to cluster

PUTP_STAA8 / Q2FWY7 Sodium/proline symporter; Proline permease from Staphylococcus aureus (strain NCTC 8325)
TC 2.A.21.2.2 / Q7A4Q7 Sodium/proline symporter (Proline permease) from Staphylococcus aureus (strain N315)
PFams: SSF
512 amino acids: PaperBLAST, CDD, Compare to cluster

TC 2.A.21.2.6 / O24896 High affinity proline-specific Na+:proline symporter, PutP (Rivera-Ordaz et al. 2013).  Proline is a preferred source of energy for this microaerophilic bacterium.  PutP is efficiently inhibited by the proline analogs, 3,4-dehydro-D,L-proline and L-azetidine-2-carboxylic acid from Helicobacter pylori (strain ATCC 700392 / 26695)
PFams: SSF
496 amino acids: PaperBLAST, CDD, Compare to cluster

TC 2.A.21.2.3 / Q9I5F5 L-proline uptake porter, PutP from Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
PFams: SSF
506 amino acids: PaperBLAST, CDD, Compare to cluster