Other Characterized Proteins Similar to prpF

GapMind for catabolism of small carbon sources

Other Characterized Proteins Similar to prpF

Finding the characterized proteins for prpF (methylaconitate isomerase) in propionate catabolism

Or see clustering for step prpF

Or see all steps for propionate catabolism

Or cluster curated proteins matching a keyword

Definition of prpF

Curated proteins or TIGRFams with EC 5.3.3.7
Curated sequence Q937N7: 2-methyl-aconitate isomerase; Cis-trans isomerase; EC 5.3.3.-
Ignore hits to A0A0A1H8I4 when looking for 'other' hits (Aconitate isomerase; AI; EC 5.3.3.7. aconitate DELTA-isomerase (EC 5.3.3.7))
Comment: SwissProt annotates Q937N7 with this activity, but with a vague EC number. The periplasmic substrate-binding protein A0A0A1H8I4 has aconitate isomerase activity but has a high Km (PMID:26293748), and its periplasmic location would prevent it from participating in the methylcitrate cycle, so it is ignored.

Fetched 3 sequences

Running ublast to find other characterized proteins with 40% identity and 70% coverage

Found hits to 3 other characterized sequences. (Found 6 hits including self hits.)

Close sequences

(Sequences that are similar to these will not be high-confidence candidates for prpF.)

Q0QLE6 3-methylitaconate Δ-isomerase (EC 5.3.3.6) from Eubacterium barkeri
MII_EUBBA / Q0QLE6 3-methylitaconate isomerase; (R)-3-methylitaconate isomerase; 3-methylitaconate delta-isomerase; EC 5.3.3.6 from Eubacterium barkeri (Clostridium barkeri)
Q0QLE6 methylitaconate DELTA-isomerase (EC 5.3.3.6) from Eubacterium barkeri
PFams: PrpF
380 amino acids: PaperBLAST, CDD, SwissProt::Q0QLE6
40% identical to

PRPF_CUPNE / Q937N7 2-methyl-aconitate isomerase; Cis-trans isomerase; EC 5.3.3.- from Cupriavidus necator (Alcaligenes eutrophus) (Ralstonia eutropha)
PFams: PrpF
396 amino acids: PaperBLAST, CDD

Q88JY0 4-oxalomesaconate tautomerase (EC 5.3.2.8) from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)
GALD_PSEPK / Q88JY0 4-oxalomesaconate tautomerase; Gallate degradation protein D; EC 5.3.2.8 from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)
PFams: PrpF
361 amino acids: PaperBLAST, CDD, SwissProt::Q88JY0
44% identical to

A0A0U2X0E4 aconitate-Δ-isomerase 1 (EC 5.3.3.7) from Ustilago maydis
ADI1_USTMD / A0A0U2X0E4 Aconitate-delta-isomerase 1; Itaconic acid/2-hydroxyparaconate biosynthesis cluster protein ADI1; EC 5.-.-.- from Ustilago maydis (Corn smut fungus)
PFams: PrpF
443 amino acids: PaperBLAST, CDD

LIGU_NOVK1 / Q0KJL4 (4E)-oxalomesaconate Delta-isomerase; OMA isomerase; 1,3-allylic isomerase LigU; EC 5.3.3.- from Novosphingobium sp. (strain KA1) (Sphingomonas sp. (strain KA1))
PFams: PrpF
357 amino acids: PaperBLAST, CDD, SwissProt::Q0KJL4
41% identical to