Other Characterized Proteins Similar to glk

GapMind for catabolism of small carbon sources

Other Characterized Proteins Similar to glk

Finding the characterized proteins for glk (glucokinase) in sucrose catabolism

Or see clustering for step glk

Or see all steps for sucrose catabolism

Or cluster curated proteins matching a keyword

Definition of glk

Curated proteins or TIGRFams with EC 2.7.1.1
Curated proteins or TIGRFams with EC 2.7.1.2
UniProt sequence A8LLL9: SubName: Full=Glucokinase {ECO:0000313|EMBL:ABV93397.1}; EC=2.7.1.2 {ECO:0000313|EMBL:ABV93397.1};
UniProt sequence Q5GAN8: SubName: Full=General specificity hexokinase {ECO:0000313|EMBL:AAT74901.1};
Curated sequence MONOMER-19002: N-acetylglucosamine kinase (EC 2.7.1.59)
Ignore hits to CH_123431 when looking for 'other' hits (N-acetylglucosamine kinase)
Ignore hits to Q9ADE8 when looking for 'other' hits (polyphosphate-glucose phosphotransferase (EC 2.7.1.63))
UniProt sequence I7DXX1: SubName: Full=Glucokinase Glk {ECO:0000313|EMBL:AFO90274.1}; EC=2.7.1.2 {ECO:0000313|EMBL:AFO90274.1};
Comment: Dshi_1655 (A8LLL9) was identified using fitness data, is very important for growth on glucose and several glucose-containing disaccharides; and functional residues seem to be conserved. The related protein PGA1_c05420 (I7DXX1) also seems to be glk. Q5GAN8 = hexA from B. fragilis is a broad-specificity hexokinase (PMID:15659667). Q5GBH5 = rokA = MONOMER-19002 from B. fragilis is a glucose/mannose/NAG kinase (PMID:15659667). (MetaCyc lists it as NAG kinase only but it is just as active on glucose.) CH_123431 is nearly identical to a characterized broad-specificity hexose kinase, so ignore. SCO5059 (Q9ADE8) is reported to have ATP-glucokinase activity (PMID:24200789), so ignore.

Fetched 98 sequences

Running ublast to find other characterized proteins with 40% identity and 70% coverage

Found hits to 8 other characterized sequences. (Found 103 hits including self hits.)

Removing ignored items reduces this to 6 close sequences.

Close sequences

(Sequences that are similar to these will not be high-confidence candidates for glk.)

b0394 fructokinase (EC 2.7.1.7; EC 2.7.1.4) from Escherichia coli K-12 substr. MG1655
P23917 fructokinase (EC 2.7.1.7; EC 2.7.1.4) from Escherichia coli (strain K12)
MAK_ECOLI / P23917 Fructokinase; D-fructose kinase; Manno(fructo)kinase; EC 2.7.1.4 from Escherichia coli (strain K12)
PFams: ROK, Glucokinase
302 amino acids: PaperBLAST, CDD, SwissProt::P23917
59% identical to

Sama_2231 glucokinase (EC 2.7.1.2) from Shewanella amazonensis SB2B
PFams: ROK, Glucokinase
299 amino acids: PaperBLAST, CDD

SMc03109 Mannokinase (EC 2.7.1.7) from Sinorhizobium meliloti 1021
PFams: ROK
298 amino acids: PaperBLAST, CDD, reanno::Smeli:SMc03109
52% identical to

Sama_2231 glucokinase (EC 2.7.1.2) from Shewanella amazonensis SB2B
PFams: ROK, Glucokinase
299 amino acids: PaperBLAST, CDD

Q76LT2 polyphosphate-dependent glucokinase subunit (EC 2.7.1.63) from Microlunatus phosphovorus
PFams: ROK
266 amino acids: PaperBLAST, CDD, metacyc::MONOMER-6061
50% identical to

P9WIN1 polyphosphate-dependent glucokinase (EC 2.7.1.63) from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
PPGK_MYCTA / A5U654 Polyphosphate glucokinase; Poly(P) glucokinase; ATP-dependent glucokinase; Polyphosphate--glucose phosphotransferase; EC 2.7.1.63; EC 2.7.1.2 from Mycobacterium tuberculosis (strain ATCC 25177 / H37Ra)
PPGK_MYCTU / P9WIN1 Polyphosphate glucokinase; Poly(P) glucokinase; ATP-dependent glucokinase; Polyphosphate--glucose phosphotransferase; EC 2.7.1.63; EC 2.7.1.2 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
PFams: ROK
265 amino acids: PaperBLAST, CDD

b1119 N-acetyl-D-glucosamine kinase (EC 2.7.1.59) from Escherichia coli K-12 substr. MG1655
P75959 N-acetyl-D-glucosamine kinase (EC 2.7.1.59) from Escherichia coli (strain K12)
NAGK_ECOLI / P75959 N-acetyl-D-glucosamine kinase; GlcNAc kinase; EC 2.7.1.59 from Escherichia coli (strain K12)
GB|ABB62126.1 N-acetyl-D-glucosamine kinase; EC 2.7.1.59 from Escherichia coli K12
PFams: ROK, Glucokinase
303 amino acids: PaperBLAST, CDD, CharProtDB::CH_002679
42% identical to

Sama_2231 glucokinase (EC 2.7.1.2) from Shewanella amazonensis SB2B
PFams: ROK, Glucokinase
299 amino acids: PaperBLAST, CDD

Q8R8N4 ADP-specific glucokinase (EC 2.7.1.147) from Caldanaerobacter subterraneus subsp. tengcongensis
PFams: ROK
312 amino acids: PaperBLAST, CDD, BRENDA::Q8R8N4
41% identical to

Q8RDE9 glucokinase (EC 2.7.1.2) from Caldanaerobacter subterraneus subsp. tengcongensis
PFams: ROK
315 amino acids: PaperBLAST, CDD

NAGK_SALTY / Q8ZPZ9 N-acetyl-D-glucosamine kinase; GlcNAc kinase; EC 2.7.1.59 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
PFams: ROK, Glucokinase
303 amino acids: PaperBLAST, CDD, SwissProt::Q8ZPZ9
40% identical to

Sama_2231 glucokinase (EC 2.7.1.2) from Shewanella amazonensis SB2B
PFams: ROK, Glucokinase
299 amino acids: PaperBLAST, CDD

Close but ignored sequences

(Sequences that are similar to these will still be high-confidence candidates for glk.)

CH_123431 N-acetylglucosamine kinase from Candida albicans
PFams: Hexokinase_1, Hexokinase_2
493 amino acids: PaperBLAST, CDD, CharProtDB::CH_123431
99% identical to

HXK1_CANAL / Q59RW5 N-acetylglucosamine kinase 1; GlcNAc kinase 1; Hexokinase 1; EC 2.7.1.59; EC 2.7.1.1 from Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast)
PFams: Hexokinase_1, Hexokinase_2
493 amino acids: PaperBLAST, CDD

Q9ADE8 polyphosphate-glucose phosphotransferase (EC 2.7.1.63) from Streptomyces coelicolor
PFams: ROK
246 amino acids: PaperBLAST, CDD, BRENDA::Q9ADE8
57% identical to