Clusters of Characterized Proteins

Clustering the characterized proteins for nupC (thymidine permease NupC) in thymidine catabolism

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Definition of nupC

• Curated sequence P0AFF2: Nucleoside permease NupC; Nucleoside-transport system protein NupC. Pyrimidine nucleoside:H+ symporter, NupC (Craig et al. 1994; Patching et al. 2005). Wild-type NupC had an apparent affinity for uridine of 22.2 +/- 3.7 muM and an apparent binding affinity of 1.8-2.6 mM, and various mutants with alterred properties were isolated and characterized (Sun et al. 2017). ADP-glucose is also a substrate of this system. nucleoside:H⁺ symporter NupC. nucleoside:H⁺ symporter NupC
• Ignore hits to P39141 when looking for 'other' hits (Nucleoside permease NupC. Pyrimidine-specific nucleoside:H+ symporter, NupC)
• UniProt sequence A0KU05: RecName: Full=Nucleoside permease {ECO:0000256|RuleBase:RU362018}; Flags: Precursor;
• Ignore hits to Q9KPL5 when looking for 'other' hits (Concentrative nucleoside transporter, CNT, of 418 aas and 12 TMSs. A repeat-swapped model of VcCNT predicts that nucleoside transport occurs via a mechanism involving an elevator-like substrate binding domain movement across the membrane)
• Ignore hits to P33021 when looking for 'other' hits (Putative nucleoside permease NupX. Nucleoside permease NupX. putative nucleoside transporter)
• Comment: A nupC-like protein from Shewanella sp. ANA-3 (Shewana3_1039, A0KU05) is important for utilization of thymidine and other nucleosides. A similar protein from V. cholerae (Q9KPL5) binds uridine and 2'-deoxyuridine and is likely to be a thymidine transporter as well, but this is not proven. The nupC protein from B. subtilis (P39141) was shown to be a uridine transporter (PMID:8550462) and is suspected to be a thymidine transporter as well, so it is ignored. The specificity of E. coli nupX (P33021, also known as yeiJ) seems to be unknown.

Fetched 2 sequences

Running BLASTp

Found similarities, at above 30% identity and 75% coverage, for 1 of these sequences

Found 1 clusters of similar sequences. Download as table or as draft rules or view by organism

Cluster 1 400-419 amino acids (not heteromeric)

A0KU05 Nucleoside permease {ECO:0000256|RuleBase:RU362018}; Flags: Precursor
419 amino acids: PaperBLAST, CDD, Compare to cluster

b2393 nucleoside:H⁺ symporter NupC from Escherichia coli K-12 substr. MG1655
P0AFF2 nucleoside:H⁺ symporter NupC from Escherichia coli (strain K12)
NUPC_ECOLI / P0AFF2 Nucleoside permease NupC; Nucleoside-transport system protein NupC from Escherichia coli (strain K12)
TC 2.A.41.1.1 / P0AFF2 Pyrimidine nucleoside:H+ symporter, NupC (Craig et al. 1994; Patching et al. 2005). Wild-type NupC had an apparent affinity for uridine of 22.2 +/- 3.7 muM and an apparent binding affinity of 1.8-2.6 mM, and various mutants with alterred properties were isolated and characterized (Sun et al. 2017). ADP-glucose is also a substrate of this system from Escherichia coli
PFams: Nucleos_tra2_N, Gate, Nucleos_tra2_C
400 amino acids: PaperBLAST, CDD, Compare to cluster