Other Characterized Proteins Similar to nupC

Finding the characterized proteins for nupC (thymidine permease NupC) in thymidine catabolism

Or see clustering for step nupC

Or see all steps for thymidine catabolism

Or cluster curated proteins matching a keyword

Definition of nupC

• Curated sequence P0AFF2: Nucleoside permease NupC; Nucleoside-transport system protein NupC. Pyrimidine nucleoside:H+ symporter, NupC (Craig et al. 1994; Patching et al. 2005). Wild-type NupC had an apparent affinity for uridine of 22.2 +/- 3.7 muM and an apparent binding affinity of 1.8-2.6 mM, and various mutants with alterred properties were isolated and characterized (Sun et al. 2017). ADP-glucose is also a substrate of this system. nucleoside:H⁺ symporter NupC. nucleoside:H⁺ symporter NupC
• Ignore hits to P39141 when looking for 'other' hits (Nucleoside permease NupC. Pyrimidine-specific nucleoside:H+ symporter, NupC)
• UniProt sequence A0KU05: RecName: Full=Nucleoside permease {ECO:0000256|RuleBase:RU362018}; Flags: Precursor;
• Ignore hits to Q9KPL5 when looking for 'other' hits (Concentrative nucleoside transporter, CNT, of 418 aas and 12 TMSs. A repeat-swapped model of VcCNT predicts that nucleoside transport occurs via a mechanism involving an elevator-like substrate binding domain movement across the membrane)
• Ignore hits to P33021 when looking for 'other' hits (Putative nucleoside permease NupX. Nucleoside permease NupX. putative nucleoside transporter)
• Comment: A nupC-like protein from Shewanella sp. ANA-3 (Shewana3_1039, A0KU05) is important for utilization of thymidine and other nucleosides. A similar protein from V. cholerae (Q9KPL5) binds uridine and 2'-deoxyuridine and is likely to be a thymidine transporter as well, but this is not proven. The nupC protein from B. subtilis (P39141) was shown to be a uridine transporter (PMID:8550462) and is suspected to be a thymidine transporter as well, so it is ignored. The specificity of E. coli nupX (P33021, also known as yeiJ) seems to be unknown.

Fetched 2 sequences

Running ublast to find other characterized proteins with 40% identity and 70% coverage

Found hits to 4 other characterized sequences. (Found 5 hits including self hits.)

Removing ignored items reduces this to 1 close sequences.

Close sequences

(Sequences that are similar to these will not be high-confidence candidates for nupC.)

NUPC_HELPY / O25792 Nucleoside permease NupC from Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
PFams: Nucleos_tra2_N, Nucleos_tra2_C
418 amino acids: PaperBLAST, CDD, SwissProt::O25792
53% identical to

A0KU05 Nucleoside permease {ECO:0000256|RuleBase:RU362018}; Flags: Precursor
419 amino acids: PaperBLAST, CDD

Close but ignored sequences

(Sequences that are similar to these will still be high-confidence candidates for nupC.)

TC 2.A.41.2.11 / Q9KPL5 Concentrative nucleoside transporter, CNT, of 418 aas and 12 TMSs. A repeat-swapped model of VcCNT predicts that nucleoside transport occurs via a mechanism involving an elevator-like substrate binding domain movement across the membrane from Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
PFams: Nucleos_tra2_N, Gate, Nucleos_tra2_C
418 amino acids: PaperBLAST, CDD, TCDB::Q9KPL5
67% identical to

A0KU05 Nucleoside permease {ECO:0000256|RuleBase:RU362018}; Flags: Precursor
419 amino acids: PaperBLAST, CDD

NUPC_BACSU / P39141 Nucleoside permease NupC from Bacillus subtilis (strain 168)
TC 2.A.41.1.2 / P39141 Pyrimidine-specific nucleoside:H+ symporter, NupC from Bacillus subtilis
PFams: Nucleos_tra2_N, Nucleos_tra2_C
393 amino acids: PaperBLAST, CDD, SwissProt::P39141
60% identical to

b2393 nucleoside:H⁺ symporter NupC from Escherichia coli K-12 substr. MG1655
P0AFF2 nucleoside:H⁺ symporter NupC from Escherichia coli (strain K12)
NUPC_ECOLI / P0AFF2 Nucleoside permease NupC; Nucleoside-transport system protein NupC from Escherichia coli (strain K12)
TC 2.A.41.1.1 / P0AFF2 Pyrimidine nucleoside:H+ symporter, NupC (Craig et al. 1994; Patching et al. 2005). Wild-type NupC had an apparent affinity for uridine of 22.2 +/- 3.7 muM and an apparent binding affinity of 1.8-2.6 mM, and various mutants with alterred properties were isolated and characterized (Sun et al. 2017). ADP-glucose is also a substrate of this system from Escherichia coli
PFams: Nucleos_tra2_N, Gate, Nucleos_tra2_C
400 amino acids: PaperBLAST, CDD

P33021 putative nucleoside transporter from Escherichia coli (strain K12)
NUPX_ECOLI / P33021 Putative nucleoside permease NupX from Escherichia coli (strain K12)
TC 2.A.41.2.10 / P33021 Nucleoside permease NupX from Escherichia coli (strain K12)
PFams: Nucleos_tra2_N, Nucleos_tra2_C
416 amino acids: PaperBLAST, CDD, SwissProt::P33021
49% identical to

A0KU05 Nucleoside permease {ECO:0000256|RuleBase:RU362018}; Flags: Precursor
419 amino acids: PaperBLAST, CDD