Potential Gaps in catabolism of small carbon sources in Imtechella halotolerans K1
Found 128 low-confidence and 30 medium-confidence steps on the best paths for 62 pathways.
| Pathway | Step | Best candidate | 2nd candidate |
|---|
| 2-oxoglutarate | kgtP: 2-oxoglutarate:H+ symporter KgtP | | |
| 4-hydroxybenzoate | mhpD: 2-hydroxypentadienoate hydratase | | |
| 4-hydroxybenzoate | mhpE: 4-hydroxy-2-oxovalerate aldolase | W5A_RS12300 | |
| 4-hydroxybenzoate | pcaK: 4-hydroxybenzoate transporter pcaK | | |
| 4-hydroxybenzoate | pobA: 4-hydroxybenzoate 3-monooxygenase | | |
| 4-hydroxybenzoate | praA: protocatechuate 2,3-dioxygenase | | |
| 4-hydroxybenzoate | xylF: 2-hydroxymuconate semialdehyde hydrolase | | |
| acetate | actP: cation/acetate symporter ActP | | |
| arabinose | araA: L-arabinose isomerase | | |
| arabinose | araB: ribulokinase | | |
| arabinose | araD: L-ribulose-5-phosphate epimerase | | |
| arginine | rocE: L-arginine permease | | |
| arginine | rocF: arginase | | |
| cellobiose | glk: glucokinase | | |
| citrate | SLC13A5: citrate:Na+ symporter | W5A_RS04570 | |
| citrulline | AO353_03040: ABC transporter for L-Citrulline, ATPase component | W5A_RS09855 | W5A_RS05890 |
| citrulline | AO353_03045: ABC transporter for L-Citrulline, permease component 2 | | |
| citrulline | AO353_03050: ABC transporter for L-Citrulline, permease component 1 | | |
| citrulline | AO353_03055: ABC transporter for L-Citrulline, periplasmic substrate-binding component | | |
| D-alanine | cycA: D-alanine:H+ symporter CycA | | |
| D-alanine | dadA: D-alanine dehydrogenase | | |
| D-lactate | D-LDH: D-lactate dehydrogenase | W5A_RS03280 | W5A_RS12165 |
| D-lactate | lctP: D-lactate:H+ symporter LctP or LidP | | |
| D-serine | cycA: D-serine:H+ symporter CycA | | |
| D-serine | dsdA: D-serine ammonia-lyase | W5A_RS12330 | |
| deoxyinosine | deoB: phosphopentomutase | W5A_RS11005 | |
| deoxyribonate | deoxyribonate-dehyd: 2-deoxy-D-ribonate 3-dehydrogenase | W5A_RS11970 | W5A_RS09265 |
| deoxyribonate | deoxyribonate-transport: 2-deoxy-D-ribonate transporter | | |
| deoxyribonate | garK: glycerate 2-kinase | | |
| deoxyribonate | ketodeoxyribonate-cleavage: 2-deoxy-3-keto-D-ribonate cleavage enzyme | | |
| deoxyribose | deoK: deoxyribokinase | | |
| deoxyribose | deoP: deoxyribose transporter | W5A_RS09270 | |
| ethanol | etoh-dh-nad: ethanol dehydrogenase (NAD(P)) | W5A_RS13360 | W5A_RS10270 |
| fructose | 1pfk: 1-phosphofructokinase | | |
| fructose | fruII-ABC: fructose-specific PTS system (fructose 1-phosphate forming), EII-ABC components | | |
| fucose | fucD: L-fuconate dehydratase | | |
| fucose | fucDH: 2-keto-3-deoxy-L-fuconate 4-dehydrogenase | W5A_RS09235 | W5A_RS11970 |
| fucose | fuconolactonase: L-fucono-1,5-lactonase | W5A_RS09245 | |
| galacturonate | eda: 2-keto-3-deoxygluconate 6-phosphate aldolase | | |
| galacturonate | exuT: D-galacturonate transporter ExuT | | |
| galacturonate | kdgK: 2-keto-3-deoxygluconate kinase | | |
| galacturonate | uxaB: tagaturonate reductase | | |
| galacturonate | uxaC: D-galacturonate isomerase | | |
| gluconate | gnd: 6-phosphogluconate dehydrogenase, decarboxylating | | |
| gluconate | gntK: D-gluconate kinase | | |
| gluconate | gntT: gluconate:H+ symporter GntT | | |
| glucosamine | gamP: glucosamine PTS system, EII-CBA components (GamP/NagE) | | |
| glucose | glk: glucokinase | | |
| glucose-6-P | uhpT: glucose-6-phosphate:phosphate antiporter | | |
| glucuronate | exuT: D-glucuronate:H+ symporter ExuT | | |
| glucuronate | gci: D-glucaro-1,4-lactone cycloisomerase | | |
| glucuronate | kdgD: 5-dehydro-4-deoxyglucarate dehydratase | | |
| glucuronate | udh: D-glucuronate dehydrogenase | | |
| glycerol | glpD: glycerol 3-phosphate dehydrogenase (monomeric) | | |
| glycerol | glpF: glycerol facilitator glpF | W5A_RS10955 | |
| glycerol | glpK: glycerol kinase | | |
| histidine | hutG: N-formiminoglutamate formiminohydrolase | W5A_RS11270 | |
| histidine | permease: L-histidine permease | | |
| isoleucine | acdH: (2S)-2-methylbutanoyl-CoA dehydrogenase | W5A_RS06285 | W5A_RS05590 |
| isoleucine | bkdA: branched-chain alpha-ketoacid dehydrogenase, E1 component alpha subunit | W5A_RS11795 | W5A_RS08900 |
| isoleucine | bkdB: branched-chain alpha-ketoacid dehydrogenase, E1 component beta subunit | W5A_RS02640 | W5A_RS11795 |
| isoleucine | bkdC: branched-chain alpha-ketoacid dehydrogenase, E2 component | W5A_RS08895 | W5A_RS05190 |
| isoleucine | brnQ: L-isoleucine:cation symporter BrnQ/BraZ/BraB | W5A_RS01735 | |
| isoleucine | ech: 2-methyl-3-hydroxybutyryl-CoA hydro-lyase | W5A_RS03060 | W5A_RS07185 |
| isoleucine | ivdG: 3-hydroxy-2-methylbutyryl-CoA dehydrogenase | W5A_RS11970 | W5A_RS10270 |
| isoleucine | pccA: propionyl-CoA carboxylase, alpha subunit | W5A_RS01050 | |
| L-lactate | lctO: L-lactate oxidase or 2-monooxygenase | W5A_RS09285 | |
| L-lactate | lctP: L-lactate:H+ symporter LctP or LidP | | |
| lactose | glk: glucokinase | | |
| lactose | lacP: lactose permease LacP | | |
| leucine | bkdA: branched-chain alpha-ketoacid dehydrogenase, E1 component alpha subunit | W5A_RS11795 | W5A_RS08900 |
| leucine | bkdB: branched-chain alpha-ketoacid dehydrogenase, E1 component beta subunit | W5A_RS02640 | W5A_RS11795 |
| leucine | bkdC: branched-chain alpha-ketoacid dehydrogenase, E2 component | W5A_RS08895 | W5A_RS05190 |
| leucine | brnQ: L-leucine:Na+ symporter BrnQ/BraB | W5A_RS01735 | |
| leucine | liuA: isovaleryl-CoA dehydrogenase | W5A_RS05590 | W5A_RS06285 |
| leucine | liuB: 3-methylcrotonyl-CoA carboxylase, alpha (biotin-containing) subunit | W5A_RS01050 | |
| leucine | liuC: 3-methylglutaconyl-CoA hydratase | W5A_RS03060 | W5A_RS01130 |
| leucine | liuD: 3-methylcrotonyl-CoA carboxylase, beta subunit | W5A_RS11335 | |
| lysine | hglS: D-2-hydroxyglutarate synthase | | |
| lysine | lat: L-lysine 6-aminotransferase | W5A_RS07400 | W5A_RS06850 |
| lysine | lysN: 2-aminoadipate transaminase | W5A_RS06160 | W5A_RS06850 |
| lysine | lysP: L-lysine:H+ symporter LysP | | |
| lysine | ydiJ: (R)-2-hydroxyglutarate dehydrogenase | W5A_RS11720 | |
| maltose | glk: glucokinase | | |
| mannitol | mtlA: mannitol phosphotransferase system, EII-CBA components | | |
| mannitol | mtlD: mannitol-1-phosphate 5-dehydrogenase | | |
| mannose | manA: mannose-6-phosphate isomerase | W5A_RS05415 | W5A_RS11640 |
| mannose | manP: mannose PTS system, EII-CBA components | | |
| myoinositol | iolB: 5-deoxy-D-glucuronate isomerase | | |
| myoinositol | iolC: 5-dehydro-2-deoxy-D-gluconate kinase | | |
| myoinositol | iolD: 3D-(3,5/4)-trihydroxycyclohexane-1,2-dione hydrolase | | |
| myoinositol | iolE: scyllo-inosose 2-dehydratase | | |
| myoinositol | iolG: myo-inositol 2-dehydrogenase | | |
| myoinositol | iolJ: 5-dehydro-2-deoxyphosphogluconate aldolase | W5A_RS02565 | |
| myoinositol | iolT: myo-inositol:H+ symporter | | |
| myoinositol | mmsA: malonate-semialdehyde dehydrogenase | W5A_RS05220 | |
| NAG | nagEcba: N-acetylglucosamine phosphotransferase system, EII-CBA components | | |
| phenylacetate | paaA: phenylacetyl-CoA 1,2-epoxidase, subunit A | | |
| phenylacetate | paaB: phenylacetyl-CoA 1,2-epoxidase, subunit B | | |
| phenylacetate | paaC: phenylacetyl-CoA 1,2-epoxidase, subunit C | | |
| phenylacetate | paaF: 2,3-dehydroadipyl-CoA hydratase | W5A_RS03060 | W5A_RS07185 |
| phenylacetate | paaG: 1,2-epoxyphenylacetyl-CoA isomerase / 2-(oxepinyl)acetyl-CoA isomerase / didehydroadipyl-CoA isomerase | W5A_RS03060 | |
| phenylacetate | paaJ1: 3-oxo-5,6-dehydrosuberyl-CoA thiolase | W5A_RS07190 | W5A_RS06410 |
| phenylacetate | paaJ2: 3-oxoadipyl-CoA thiolase | W5A_RS07190 | W5A_RS06410 |
| phenylacetate | paaK: phenylacetate-CoA ligase | | |
| phenylacetate | paaT: phenylacetate transporter Paa | | |
| phenylacetate | paaZ1: oxepin-CoA hydrolase | | |
| phenylacetate | paaZ2: 3-oxo-5,6-didehydrosuberyl-CoA semialdehyde dehydrogenase | | |
| phenylalanine | aroP: L-phenylalanine:H+ symporter AroP | | |
| phenylalanine | maiA: maleylacetoacetate isomerase | | |
| phenylalanine | PAH: phenylalanine 4-monooxygenase | W5A_RS04525 | |
| phenylalanine | PCBD: pterin-4-alpha-carbinoalamine dehydratase | | |
| phenylalanine | QDPR: 6,7-dihydropteridine reductase | | |
| proline | proY: proline:H+ symporter | | |
| propionate | pccA: propionyl-CoA carboxylase, alpha subunit | W5A_RS01050 | |
| propionate | putP: propionate transporter; proline:Na+ symporter | | |
| putrescine | gabD: succinate semialdehyde dehydrogenase | W5A_RS05220 | W5A_RS01275 |
| putrescine | gabT: gamma-aminobutyrate transaminase | W5A_RS12435 | W5A_RS06850 |
| putrescine | patA: putrescine aminotransferase (PatA/SpuC) | W5A_RS06850 | W5A_RS07400 |
| putrescine | patD: gamma-aminobutyraldehyde dehydrogenase | W5A_RS05220 | W5A_RS12355 |
| putrescine | puuP: putrescine:H+ symporter PuuP/PlaP | | |
| pyruvate | SLC5A8: sodium-coupled pyruvate transporter | W5A_RS03140 | |
| rhamnose | LRA1: L-rhamnofuranose dehydrogenase | W5A_RS11970 | W5A_RS10270 |
| rhamnose | LRA2: L-rhamnono-gamma-lactonase | | |
| rhamnose | LRA3: L-rhamnonate dehydratase | | |
| rhamnose | LRA5: 2-keto-3-deoxy-L-rhamnonate 4-dehydrogenase | W5A_RS10270 | W5A_RS11970 |
| rhamnose | rhaT: L-rhamnose:H+ symporter RhaT | | |
| ribose | rbsK: ribokinase | | |
| ribose | rbsU: probable D-ribose transporter RbsU | | |
| serine | serP: L-serine permease SerP | | |
| sorbitol | mtlA: PTS system for polyols, EII-CBA components | | |
| sorbitol | srlD: sorbitol 6-phosphate 2-dehydrogenase | W5A_RS11970 | W5A_RS09265 |
| sucrose | ams: sucrose hydrolase (invertase) | | |
| sucrose | glk: glucokinase | | |
| threonine | tdcC: L-threonine:H+ symporter TdcC | | |
| thymidine | deoA: thymidine phosphorylase DeoA | | |
| thymidine | deoB: phosphopentomutase | W5A_RS11005 | |
| thymidine | nupG: thymidine permease NupG/XapB | | |
| trehalose | glk: glucokinase | | |
| trehalose | treF: trehalase | | |
| tryptophan | aroP: tryptophan:H+ symporter AroP | | |
| tryptophan | tnaA: tryptophanase | | |
| tyrosine | aroP: L-tyrosine transporter (AroP/FywP) | | |
| tyrosine | maiA: maleylacetoacetate isomerase | | |
| valine | acdH: isobutyryl-CoA dehydrogenase | W5A_RS06285 | W5A_RS05590 |
| valine | bch: 3-hydroxyisobutyryl-CoA hydrolase | W5A_RS03060 | |
| valine | bkdA: branched-chain alpha-ketoacid dehydrogenase, E1 component alpha subunit | W5A_RS11795 | W5A_RS08900 |
| valine | bkdB: branched-chain alpha-ketoacid dehydrogenase, E1 component beta subunit | W5A_RS02640 | W5A_RS11795 |
| valine | bkdC: branched-chain alpha-ketoacid dehydrogenase, E2 component | W5A_RS08895 | W5A_RS05190 |
| valine | brnQ: L-valine:cation symporter BrnQ/BraZ/BraB | W5A_RS01735 | |
| valine | ech: (S)-3-hydroxybutanoyl-CoA hydro-lyase | W5A_RS03060 | W5A_RS07185 |
| valine | mmsA: methylmalonate-semialdehyde dehydrogenase | W5A_RS05220 | W5A_RS01275 |
| valine | mmsB: 3-hydroxyisobutyrate dehydrogenase | | |
| valine | pccA: propionyl-CoA carboxylase, alpha subunit | W5A_RS01050 | |
| xylitol | fruI: xylitol PTS, enzyme IIABC (FruI) | | |
| xylitol | x5p-reductase: D-xylulose-5-phosphate 2-reductase | | |
| xylose | xylA: xylose isomerase | | |
| xylose | xylB: xylulokinase | | |
Confidence: high confidence medium confidence low confidence
This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.
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About GapMind
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using
ublast (a fast alternative to protein BLAST)
against a database of manually-curated proteins (most of which are experimentally characterized) or by using
HMMer with enzyme models (usually from
TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
- ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
- HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.
where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").
Otherwise, a candidate is "medium confidence" if either:
- ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
- ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
- HMMer finds a hit (regardless of coverage or other bits).
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps."
For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways.
For diverse bacteria and archaea that can utilize a carbon source, there is a complete
high-confidence catabolic pathway (including a transporter) just 38% of the time, and
there is a complete medium-confidence pathway 63% of the time.
Gaps may be due to:
- our ignorance of proteins' functions,
- omissions in the gene models,
- frame-shift errors in the genome sequence, or
- the organism lacks the pathway.
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory