Potential Gaps in catabolism of small carbon sources in Nafulsella turpanensis ZLM-10
Found 102 low-confidence and 43 medium-confidence steps on the best paths for 62 pathways.
| Pathway | Step | Best candidate | 2nd candidate |
|---|
| 2-oxoglutarate | kgtP: 2-oxoglutarate:H+ symporter KgtP | | |
| 4-hydroxybenzoate | mhpD: 2-hydroxypentadienoate hydratase | | |
| 4-hydroxybenzoate | mhpE: 4-hydroxy-2-oxovalerate aldolase | | |
| 4-hydroxybenzoate | pcaK: 4-hydroxybenzoate transporter pcaK | | |
| 4-hydroxybenzoate | pobA: 4-hydroxybenzoate 3-monooxygenase | | |
| 4-hydroxybenzoate | praA: protocatechuate 2,3-dioxygenase | | |
| 4-hydroxybenzoate | xylF: 2-hydroxymuconate semialdehyde hydrolase | | |
| acetate | actP: cation/acetate symporter ActP | | |
| arabinose | araA: L-arabinose isomerase | | |
| arabinose | araB: ribulokinase | | |
| arabinose | araD: L-ribulose-5-phosphate epimerase | | |
| arabinose | araE: L-arabinose:H+ symporter | F752_RS0122200 | F752_RS0104840 |
| arginine | rocE: L-arginine permease | | |
| arginine | rocF: arginase | F752_RS0110890 | F752_RS0100840 |
| cellobiose | glk: glucokinase | F752_RS0110680 | |
| cellobiose | MFS-glucose: glucose transporter, MFS superfamily | F752_RS0122200 | F752_RS0104840 |
| citrate | SLC13A5: citrate:Na+ symporter | F752_RS0121580 | |
| citrulline | AO353_03040: ABC transporter for L-Citrulline, ATPase component | F752_RS0106200 | F752_RS0112795 |
| citrulline | AO353_03045: ABC transporter for L-Citrulline, permease component 2 | | |
| citrulline | AO353_03050: ABC transporter for L-Citrulline, permease component 1 | | |
| citrulline | AO353_03055: ABC transporter for L-Citrulline, periplasmic substrate-binding component | | |
| D-alanine | cycA: D-alanine:H+ symporter CycA | | |
| D-alanine | dadA: D-alanine dehydrogenase | | |
| D-lactate | D-LDH: D-lactate dehydrogenase | F752_RS0116650 | F752_RS0110570 |
| D-lactate | lctP: D-lactate:H+ symporter LctP or LidP | | |
| D-serine | cycA: D-serine:H+ symporter CycA | | |
| D-serine | dsdA: D-serine ammonia-lyase | F752_RS0103745 | F752_RS0103525 |
| deoxyinosine | deoB: phosphopentomutase | F752_RS0120770 | |
| deoxyribonate | deoxyribonate-dehyd: 2-deoxy-D-ribonate 3-dehydrogenase | F752_RS0114160 | F752_RS0122285 |
| deoxyribonate | deoxyribonate-transport: 2-deoxy-D-ribonate transporter | | |
| deoxyribonate | ketodeoxyribonate-cleavage: 2-deoxy-3-keto-D-ribonate cleavage enzyme | | |
| deoxyribose | deoK: deoxyribokinase | | |
| deoxyribose | deoP: deoxyribose transporter | | |
| ethanol | etoh-dh-nad: ethanol dehydrogenase (NAD(P)) | F752_RS0116570 | F752_RS0114210 |
| fructose | scrK: fructokinase | F752_RS0104800 | |
| fucose | fucA: L-fuculose-phosphate aldolase FucA | | |
| fucose | fucI: L-fucose isomerase FucI | | |
| fucose | fucK: L-fuculose kinase FucK | | |
| fucose | fucO: L-lactaldehyde reductase | F752_RS0114210 | |
| fucose | fucP: L-fucose:H+ symporter FucP | | |
| fucose | fucU: L-fucose mutarotase FucU | | |
| galactose | galK: galactokinase (-1-phosphate forming) | | |
| galactose | galP: galactose:H+ symporter GalP | F752_RS0122200 | F752_RS0104840 |
| galactose | galT: UDP-glucose:alpha-D-galactose-1-phosphate uridylyltransferase | | |
| gluconate | gntK: D-gluconate kinase | F752_RS0117690 | |
| gluconate | gntT: gluconate:H+ symporter GntT | | |
| glucosamine | gamP: glucosamine PTS system, EII-CBA components (GamP/NagE) | | |
| glucosamine | nagB: glucosamine 6-phosphate deaminase (isomerizing) | F752_RS0118615 | |
| glucose | glk: glucokinase | F752_RS0110680 | |
| glucose | MFS-glucose: glucose transporter, MFS superfamily | F752_RS0122200 | F752_RS0104840 |
| glucose-6-P | uhpT: glucose-6-phosphate:phosphate antiporter | | |
| glucuronate | exuT: D-glucuronate:H+ symporter ExuT | F752_RS0121210 | |
| glucuronate | uxuB: D-mannonate dehydrogenase | | |
| glycerol | glpD: glycerol 3-phosphate dehydrogenase (monomeric) | | |
| glycerol | glpF: glycerol facilitator glpF | | |
| glycerol | glpK: glycerol kinase | | |
| histidine | permease: L-histidine permease | | |
| isoleucine | acdH: (2S)-2-methylbutanoyl-CoA dehydrogenase | F752_RS0118655 | F752_RS0101760 |
| isoleucine | Bap2: L-isoleucine permease Bap2 | | |
| isoleucine | bkdA: branched-chain alpha-ketoacid dehydrogenase, E1 component alpha subunit | F752_RS0108360 | F752_RS0103345 |
| isoleucine | bkdC: branched-chain alpha-ketoacid dehydrogenase, E2 component | F752_RS0110830 | F752_RS0118755 |
| isoleucine | ivdG: 3-hydroxy-2-methylbutyryl-CoA dehydrogenase | F752_RS0112575 | F752_RS0114160 |
| isoleucine | pccA: propionyl-CoA carboxylase, alpha subunit | F752_RS0101295 | F752_RS0116595 |
| L-lactate | L-LDH: L-lactate dehydrogenase | F752_RS0115105 | |
| L-lactate | lctP: L-lactate:H+ symporter LctP or LidP | | |
| lactose | galK: galactokinase (-1-phosphate forming) | | |
| lactose | galT: UDP-glucose:alpha-D-galactose-1-phosphate uridylyltransferase | | |
| lactose | glk: glucokinase | F752_RS0110680 | |
| lactose | lacP: lactose permease LacP | | |
| lactose | lacZ: lactase (homomeric) | F752_RS0102810 | |
| leucine | bkdA: branched-chain alpha-ketoacid dehydrogenase, E1 component alpha subunit | F752_RS0108360 | F752_RS0103345 |
| leucine | bkdC: branched-chain alpha-ketoacid dehydrogenase, E2 component | F752_RS0110830 | F752_RS0118755 |
| leucine | liuA: isovaleryl-CoA dehydrogenase | F752_RS0101760 | F752_RS0118655 |
| leucine | liuB: 3-methylcrotonyl-CoA carboxylase, alpha (biotin-containing) subunit | F752_RS0101295 | F752_RS0103845 |
| leucine | liuD: 3-methylcrotonyl-CoA carboxylase, beta subunit | F752_RS0112535 | F752_RS0107940 |
| lysine | hglS: D-2-hydroxyglutarate synthase | | |
| lysine | lat: L-lysine 6-aminotransferase | F752_RS0110225 | |
| lysine | lysN: 2-aminoadipate transaminase | F752_RS0116115 | F752_RS0110225 |
| lysine | lysP: L-lysine:H+ symporter LysP | | |
| lysine | ydiJ: (R)-2-hydroxyglutarate dehydrogenase | F752_RS0104975 | |
| maltose | glk: glucokinase | F752_RS0110680 | |
| maltose | MFS-glucose: glucose transporter, MFS superfamily | F752_RS0122200 | F752_RS0104840 |
| mannitol | mtlA: mannitol phosphotransferase system, EII-CBA components | | |
| mannitol | mtlD: mannitol-1-phosphate 5-dehydrogenase | | |
| mannose | manA: mannose-6-phosphate isomerase | F752_RS0103050 | F752_RS0103485 |
| mannose | manP: mannose PTS system, EII-CBA components | | |
| myoinositol | iolG: myo-inositol 2-dehydrogenase | F752_RS0114695 | |
| myoinositol | iolM: 2-inosose 4-dehydrogenase | | |
| myoinositol | iolN: 2,4-diketo-inositol hydratase | | |
| myoinositol | iolO: 5-dehydro-L-gluconate epimerase | | |
| myoinositol | iolT: myo-inositol:H+ symporter | F752_RS0122200 | F752_RS0104840 |
| myoinositol | uxaE: D-tagaturonate epimerase | | |
| myoinositol | uxuB: D-mannonate dehydrogenase | | |
| NAG | nagA: N-acetylglucosamine 6-phosphate deacetylase | | |
| NAG | nagB: glucosamine 6-phosphate deaminase (isomerizing) | F752_RS0118615 | |
| NAG | nagEcba: N-acetylglucosamine phosphotransferase system, EII-CBA components | | |
| phenylacetate | paaB: phenylacetyl-CoA 1,2-epoxidase, subunit B | | |
| phenylacetate | paaC: phenylacetyl-CoA 1,2-epoxidase, subunit C | F752_RS0110050 | |
| phenylacetate | paaE: phenylacetyl-CoA 1,2-epoxidase, subunit E | F752_RS0110070 | F752_RS0106290 |
| phenylacetate | paaF: 2,3-dehydroadipyl-CoA hydratase | F752_RS0119545 | F752_RS0112240 |
| phenylacetate | paaK: phenylacetate-CoA ligase | F752_RS0105800 | |
| phenylacetate | paaT: phenylacetate transporter Paa | | |
| phenylacetate | paaZ1: oxepin-CoA hydrolase | F752_RS0119545 | F752_RS0118960 |
| phenylalanine | aroP: L-phenylalanine:H+ symporter AroP | | |
| phenylalanine | maiA: maleylacetoacetate isomerase | | |
| phenylalanine | QDPR: 6,7-dihydropteridine reductase | | |
| proline | proY: proline:H+ symporter | | |
| propionate | pccA: propionyl-CoA carboxylase, alpha subunit | F752_RS0101295 | F752_RS0116595 |
| propionate | putP: propionate transporter; proline:Na+ symporter | | |
| putrescine | gabT: gamma-aminobutyrate transaminase | F752_RS0110225 | F752_RS0110815 |
| putrescine | patA: putrescine aminotransferase (PatA/SpuC) | F752_RS0110225 | F752_RS0107785 |
| putrescine | patD: gamma-aminobutyraldehyde dehydrogenase | F752_RS0109595 | F752_RS0122315 |
| putrescine | puuP: putrescine:H+ symporter PuuP/PlaP | | |
| pyruvate | SLC5A8: sodium-coupled pyruvate transporter | F752_RS0101065 | |
| rhamnose | fucO: L-lactaldehyde reductase | F752_RS0114210 | |
| rhamnose | LRA1: L-rhamnofuranose dehydrogenase | F752_RS0114160 | F752_RS0118300 |
| rhamnose | LRA2: L-rhamnono-gamma-lactonase | | |
| rhamnose | LRA3: L-rhamnonate dehydratase | F752_RS0104150 | |
| rhamnose | LRA4: 2-keto-3-deoxy-L-rhamnonate aldolase | | |
| rhamnose | rhaT: L-rhamnose:H+ symporter RhaT | | |
| ribose | rbsK: ribokinase | | |
| ribose | rbsU: probable D-ribose transporter RbsU | | |
| serine | snatA: L-serine transporter | F752_RS0111000 | |
| sorbitol | mtlA: PTS system for polyols, EII-CBA components | | |
| sorbitol | srlD: sorbitol 6-phosphate 2-dehydrogenase | F752_RS0114160 | F752_RS0110200 |
| sucrose | scrK: fructokinase | F752_RS0104800 | |
| threonine | snatA: L-threonine transporter snatA | F752_RS0111000 | |
| thymidine | deoA: thymidine phosphorylase DeoA | | |
| thymidine | deoB: phosphopentomutase | F752_RS0120770 | |
| trehalose | glk: glucokinase | F752_RS0110680 | |
| trehalose | MFS-glucose: glucose transporter, MFS superfamily | F752_RS0122200 | F752_RS0104840 |
| tryptophan | aroP: tryptophan:H+ symporter AroP | | |
| tryptophan | tnaA: tryptophanase | | |
| tyrosine | aroP: L-tyrosine transporter (AroP/FywP) | | |
| tyrosine | maiA: maleylacetoacetate isomerase | | |
| valine | acdH: isobutyryl-CoA dehydrogenase | F752_RS0118655 | F752_RS0101760 |
| valine | Bap2: L-valine permease Bap2 | | |
| valine | bch: 3-hydroxyisobutyryl-CoA hydrolase | F752_RS0112240 | F752_RS0118960 |
| valine | bkdA: branched-chain alpha-ketoacid dehydrogenase, E1 component alpha subunit | F752_RS0108360 | F752_RS0103345 |
| valine | bkdC: branched-chain alpha-ketoacid dehydrogenase, E2 component | F752_RS0110830 | F752_RS0118755 |
| valine | mmsA: methylmalonate-semialdehyde dehydrogenase | F752_RS0109595 | F752_RS0117775 |
| valine | mmsB: 3-hydroxyisobutyrate dehydrogenase | | |
| valine | pccA: propionyl-CoA carboxylase, alpha subunit | F752_RS0101295 | F752_RS0116595 |
| xylitol | PLT5: xylitol:H+ symporter PLT5 | F752_RS0122200 | F752_RS0104840 |
| xylitol | xdhA: xylitol dehydrogenase | F752_RS0114160 | F752_RS0110200 |
Confidence: high confidence medium confidence low confidence
This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.
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About GapMind
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using
ublast (a fast alternative to protein BLAST)
against a database of manually-curated proteins (most of which are experimentally characterized) or by using
HMMer with enzyme models (usually from
TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
- ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
- HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.
where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").
Otherwise, a candidate is "medium confidence" if either:
- ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
- ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
- HMMer finds a hit (regardless of coverage or other bits).
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps."
For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways.
For diverse bacteria and archaea that can utilize a carbon source, there is a complete
high-confidence catabolic pathway (including a transporter) just 38% of the time, and
there is a complete medium-confidence pathway 63% of the time.
Gaps may be due to:
- our ignorance of proteins' functions,
- omissions in the gene models,
- frame-shift errors in the genome sequence, or
- the organism lacks the pathway.
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory