Potential Gaps in catabolism of small carbon sources in Limnohabitans parvus II-B4
Found 91 low-confidence and 58 medium-confidence steps on the best paths for 62 pathways.
Pathway | Step | Best candidate | 2nd candidate |
2-oxoglutarate | Psest_0084: 2-oxoglutarate TRAP transporter, fused 4TM/12TM components | B9Z37_RS10770 | |
2-oxoglutarate | Psest_0085: 2-oxoglutarate TRAP transporter, solute receptor component | B9Z37_RS10765 | |
4-hydroxybenzoate | ligU: 4-oxalomesaconate tautomerase | | |
4-hydroxybenzoate | pcaK: 4-hydroxybenzoate transporter pcaK | | |
alanine | braC: L-alanine/L-serine/L-threonine ABC transporter, substrate binding protein (BraC/NatB) | B9Z37_RS11950 | |
alanine | braD: L-alanine/L-serine/L-threonine ABC transporter, permease component 1 (BraD/NatD) | B9Z37_RS09270 | B9Z37_RS08650 |
alanine | braF: L-alanine/L-serine/L-threonine ABC transporter, ATP-binding component 1 (BraF/NatA) | B9Z37_RS09280 | B9Z37_RS08660 |
alanine | braG: L-alanine/L-serine/L-threonine ABC transporter, ATP-binding component 2 (BraG/NatE) | B9Z37_RS09285 | B9Z37_RS10195 |
arabinose | araE: L-arabinose:H+ symporter | | |
arabinose | xacB: L-arabinose 1-dehydrogenase | B9Z37_RS11375 | B9Z37_RS12690 |
arabinose | xacC: L-arabinono-1,4-lactonase | B9Z37_RS06620 | |
arabinose | xacE: 2-dehydro-3-deoxy-L-arabinonate dehydratase | | |
arginine | rocE: L-arginine permease | | |
arginine | rocF: arginase | | |
asparagine | aapM: ABC transporter for amino acids (Asp/Asn/Glu/Pro/Leu), permease component 2 (AapM) | B9Z37_RS12755 | |
asparagine | aapP: ABC transporter for amino acids (Asp/Asn/Glu/Pro/Leu), ATPase component AapP | B9Z37_RS12760 | B9Z37_RS06780 |
asparagine | aapQ: ABC transporter for amino acids (Asp/Asn/Glu/Pro/Leu), permease component 1 (AapQ) | B9Z37_RS12750 | |
aspartate | aapM: ABC transporter for amino acids (Asp/Asn/Glu/Pro/Leu), permease component 2 (AapM) | B9Z37_RS12755 | |
aspartate | aapP: ABC transporter for amino acids (Asp/Asn/Glu/Pro/Leu), ATPase component AapP | B9Z37_RS12760 | B9Z37_RS06780 |
aspartate | aapQ: ABC transporter for amino acids (Asp/Asn/Glu/Pro/Leu), permease component 1 (AapQ) | B9Z37_RS12750 | |
cellobiose | bgl: cellobiase | | |
citrate | SLC13A5: citrate:Na+ symporter | | |
citrulline | AO353_03040: ABC transporter for L-Citrulline, ATPase component | B9Z37_RS06780 | B9Z37_RS12760 |
citrulline | AO353_03045: ABC transporter for L-Citrulline, permease component 2 | | |
citrulline | AO353_03050: ABC transporter for L-Citrulline, permease component 1 | | |
citrulline | AO353_03055: ABC transporter for L-Citrulline, periplasmic substrate-binding component | | |
citrulline | arcC: carbamate kinase | | |
D-alanine | Pf6N2E2_5402: ABC transporter for D-Alanine, substrate-binding component | B9Z37_RS12745 | |
D-alanine | Pf6N2E2_5403: ABC transporter for D-Alanine, permease component 2 | B9Z37_RS12750 | |
D-alanine | Pf6N2E2_5404: ABC transporter for D-Alanine, permease component 1 | B9Z37_RS12755 | |
D-alanine | Pf6N2E2_5405: ABC transporter for D-Alanine, ATPase component | B9Z37_RS12760 | B9Z37_RS06780 |
D-lactate | lctP: D-lactate:H+ symporter LctP or LidP | | |
D-serine | cycA: D-serine:H+ symporter CycA | | |
D-serine | dsdA: D-serine ammonia-lyase | B9Z37_RS12805 | B9Z37_RS04975 |
deoxyinosine | deoB: phosphopentomutase | B9Z37_RS11745 | |
deoxyinosine | deoC: deoxyribose-5-phosphate aldolase | | |
deoxyinosine | deoD: deoxyinosine phosphorylase | | |
deoxyinosine | nupC: deoxyinosine:H+ symporter NupC | | |
deoxyribonate | deoxyribonate-dehyd: 2-deoxy-D-ribonate 3-dehydrogenase | B9Z37_RS12390 | B9Z37_RS00480 |
deoxyribonate | deoxyribonate-transport: 2-deoxy-D-ribonate transporter | | |
deoxyribonate | ketodeoxyribonate-cleavage: 2-deoxy-3-keto-D-ribonate cleavage enzyme | | |
deoxyribose | deoC: deoxyribose-5-phosphate aldolase | | |
deoxyribose | deoK: deoxyribokinase | B9Z37_RS02670 | |
deoxyribose | deoP: deoxyribose transporter | | |
fructose | frcB: fructose ABC transporter, substrate-binding component FrcB | | |
fructose | frcC: fructose ABC transporter, permease component FrcC | B9Z37_RS10970 | |
fructose | scrK: fructokinase | B9Z37_RS10965 | B9Z37_RS02670 |
fucose | aldA: lactaldehyde dehydrogenase | B9Z37_RS05925 | B9Z37_RS00715 |
fucose | fucA: L-fuculose-phosphate aldolase FucA | B9Z37_RS07515 | |
fucose | fucI: L-fucose isomerase FucI | | |
fucose | fucK: L-fuculose kinase FucK | | |
fucose | fucP: L-fucose:H+ symporter FucP | | |
fucose | fucU: L-fucose mutarotase FucU | B9Z37_RS10985 | |
fumarate | dauA: fumarate transporter DauA | B9Z37_RS13945 | |
galactose | dgoD: D-galactonate dehydratase | B9Z37_RS02655 | B9Z37_RS02720 |
galactose | galactonolactonase: galactonolactonase (either 1,4- or 1,5-lactone) | B9Z37_RS06620 | |
galactose | galdh: D-galactose 1-dehydrogenase (forming 1,4- or 1,5-lactones) | B9Z37_RS11375 | B9Z37_RS12690 |
galacturonate | exuT: D-galacturonate transporter ExuT | | |
galacturonate | uxuL: D-galactaro-1,5-lactonase (UxuL or UxuF) | B9Z37_RS06620 | B9Z37_RS10270 |
glucosamine | nagB: glucosamine 6-phosphate deaminase (isomerizing) | B9Z37_RS05050 | |
glucosamine | SLC2A2: glucosamine transporter SLC2A2 | | |
glucose-6-P | uhpT: glucose-6-phosphate:phosphate antiporter | | |
glucuronate | dctQ: D-glucuronate TRAP transporter, small permease component | | |
glucuronate | uxuL: D-glucaro-1,5-lactonase UxuL or UxuF | B9Z37_RS06620 | B9Z37_RS10270 |
glutamate | aapM: ABC transporter for amino acids (Asp/Asn/Glu/Pro/Leu), permease component 2 (AapM) | B9Z37_RS12755 | |
glutamate | aapP: ABC transporter for amino acids (Asp/Asn/Glu/Pro/Leu), ATPase component AapP | B9Z37_RS12760 | B9Z37_RS06780 |
glutamate | aapQ: ABC transporter for amino acids (Asp/Asn/Glu/Pro/Leu), permease component 1 (AapQ) | B9Z37_RS12750 | |
glycerol | glpD: glycerol 3-phosphate dehydrogenase (monomeric) | B9Z37_RS05785 | |
glycerol | glpF: glycerol facilitator glpF | | |
histidine | aapP: L-histidine ABC transporter, ATPase component AapP | B9Z37_RS12760 | B9Z37_RS06780 |
histidine | hutG: N-formiminoglutamate formiminohydrolase | | |
histidine | hutH: histidine ammonia-lyase | | |
histidine | hutI: imidazole-5-propionate hydrolase | | |
histidine | hutU: urocanase | | |
L-lactate | lctP: L-lactate:H+ symporter LctP or LidP | | |
L-malate | sdlC: L-malate:Na+ symporter SdlC | | |
lactose | klh: periplasmic 3'-ketolactose hydrolase | | |
lactose | lacA': periplasmic lactose 3-dehydrogenase, LacA subunit | | |
lactose | lacB': periplasmic lactose 3-dehydrogenase, cytochrome c component (LacB) | B9Z37_RS13585 | |
lactose | lacC': periplasmic lactose 3-dehydrogenase, LacC subunit | | |
lysine | davD: glutarate semialdehyde dehydrogenase | B9Z37_RS11445 | B9Z37_RS07645 |
lysine | davT: 5-aminovalerate aminotransferase | B9Z37_RS09810 | B9Z37_RS00710 |
lysine | lysP: L-lysine:H+ symporter LysP | | |
lysine | patA: cadaverine aminotransferase | B9Z37_RS09810 | B9Z37_RS00710 |
lysine | patD: 5-aminopentanal dehydrogenase | B9Z37_RS11445 | B9Z37_RS06135 |
maltose | susB: alpha-glucosidase (maltase) | | |
mannitol | mt2d: mannitol 2-dehydrogenase | B9Z37_RS12650 | B9Z37_RS02600 |
mannitol | PLT5: polyol transporter PLT5 | | |
mannitol | scrK: fructokinase | B9Z37_RS10965 | B9Z37_RS02670 |
mannose | manA: mannose-6-phosphate isomerase | | |
mannose | manP: mannose PTS system, EII-CBA components | | |
myoinositol | iolG: myo-inositol 2-dehydrogenase | | |
myoinositol | iolM: 2-inosose 4-dehydrogenase | | |
myoinositol | iolN: 2,4-diketo-inositol hydratase | | |
myoinositol | iolO: 5-dehydro-L-gluconate epimerase | | |
myoinositol | iolT: myo-inositol:H+ symporter | | |
myoinositol | uxaE: D-tagaturonate epimerase | | |
myoinositol | uxuB: D-mannonate dehydrogenase | B9Z37_RS12650 | |
NAG | nagA: N-acetylglucosamine 6-phosphate deacetylase | | |
NAG | nagB: glucosamine 6-phosphate deaminase (isomerizing) | B9Z37_RS05050 | |
NAG | nagEcba: N-acetylglucosamine phosphotransferase system, EII-CBA components | | |
phenylacetate | paaT: phenylacetate transporter Paa | | |
phenylalanine | iorAB: phenylpyruvate:ferredoxin oxidoreductase, fused IorA/IorB | B9Z37_RS07030 | |
propionate | putP: propionate transporter; proline:Na+ symporter | | |
putrescine | gabT: gamma-aminobutyrate transaminase | B9Z37_RS00710 | B9Z37_RS06015 |
putrescine | patA: putrescine aminotransferase (PatA/SpuC) | B9Z37_RS00710 | B9Z37_RS06015 |
putrescine | patD: gamma-aminobutyraldehyde dehydrogenase | B9Z37_RS00715 | B9Z37_RS05925 |
putrescine | puuP: putrescine:H+ symporter PuuP/PlaP | | |
pyruvate | dctM: pyruvate TRAP transporter, large permease component | B9Z37_RS03275 | B9Z37_RS10085 |
rhamnose | LRA1: L-rhamnofuranose dehydrogenase | B9Z37_RS01605 | B9Z37_RS02600 |
rhamnose | LRA2: L-rhamnono-gamma-lactonase | | |
rhamnose | LRA3: L-rhamnonate dehydratase | B9Z37_RS12660 | B9Z37_RS10310 |
rhamnose | rhaT: L-rhamnose:H+ symporter RhaT | | |
ribose | rbsA: D-ribose ABC transporter, ATPase component RbsA | B9Z37_RS03475 | |
ribose | rbsB: D-ribose ABC transporter, substrate-binding component RbsB | B9Z37_RS10975 | |
ribose | rbsK: ribokinase | B9Z37_RS02670 | |
serine | sdaB: L-serine ammonia-lyase | B9Z37_RS12805 | B9Z37_RS04975 |
sorbitol | scrK: fructokinase | B9Z37_RS10965 | B9Z37_RS02670 |
sorbitol | sdh: sorbitol dehydrogenase | B9Z37_RS12695 | B9Z37_RS09565 |
sorbitol | SOT: sorbitol:H+ co-transporter SOT1 or SOT2 | | |
succinate | dauA: succinate:H+ symporter DauA | B9Z37_RS13945 | |
sucrose | ams: sucrose hydrolase (invertase) | | |
threonine | braC: L-alanine/L-serine/L-threonine ABC transporter, substrate binding protein (BraC/NatB) | B9Z37_RS11950 | |
threonine | braD: L-alanine/L-serine/L-threonine ABC transporter, permease component 1 (BraD/NatD) | B9Z37_RS09270 | B9Z37_RS08650 |
threonine | braF: L-alanine/L-serine/L-threonine ABC transporter, ATP-binding component 1 (BraF/NatA) | B9Z37_RS09280 | B9Z37_RS08660 |
threonine | braG: L-alanine/L-serine/L-threonine ABC transporter, ATP-binding component 2 (BraG/NatE) | B9Z37_RS09285 | B9Z37_RS10195 |
threonine | tdcE: 2-ketobutyrate formate-lyase | | |
thymidine | deoA: thymidine phosphorylase DeoA | B9Z37_RS07680 | B9Z37_RS03025 |
thymidine | deoB: phosphopentomutase | B9Z37_RS11745 | |
thymidine | deoC: deoxyribose-5-phosphate aldolase | | |
thymidine | nupG: thymidine permease NupG/XapB | | |
trehalose | treF: trehalase | | |
tryptophan | aroP: tryptophan:H+ symporter AroP | | |
tryptophan | tnaA: tryptophanase | | |
tyrosine | hmgA: homogentisate dioxygenase | | |
tyrosine | maiA: maleylacetoacetate isomerase | B9Z37_RS14155 | B9Z37_RS13395 |
valine | livJ: L-valine ABC transporter, substrate-binding component (LivJ/LivK/BraC/BraC3) | B9Z37_RS11950 | |
valine | mmsA: methylmalonate-semialdehyde dehydrogenase | B9Z37_RS06540 | B9Z37_RS06135 |
valine | mmsB: 3-hydroxyisobutyrate dehydrogenase | B9Z37_RS07625 | B9Z37_RS06075 |
xylitol | fruI: xylitol PTS, enzyme IIABC (FruI) | | |
xylitol | x5p-reductase: D-xylulose-5-phosphate 2-reductase | | |
xylose | DKDP-dehydrog: D-2-keto-3-deoxypentoate dehydrogenase | B9Z37_RS12690 | B9Z37_RS00480 |
xylose | gtsA: xylose ABC transporter, periplasmic substrate-binding component GtsA | B9Z37_RS08260 | |
xylose | gtsB: xylose ABC transporter, permease component 1 GtsB | B9Z37_RS08265 | B9Z37_RS13745 |
xylose | gtsC: xylose ABC transporter, permease component 2 GtsC | B9Z37_RS08270 | |
xylose | gtsD: xylose ABC transporter, ATPase component GtsD | B9Z37_RS08275 | B9Z37_RS13735 |
xylose | xad: D-xylonate dehydratase | B9Z37_RS02655 | B9Z37_RS02630 |
xylose | xdh: D-xylose dehydrogenase | B9Z37_RS02600 | B9Z37_RS12390 |
xylose | xylC: xylonolactonase | B9Z37_RS06620 | |
Confidence: high confidence medium confidence low confidence
This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.
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About GapMind
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using
ublast (a fast alternative to protein BLAST)
against a database of manually-curated proteins (most of which are experimentally characterized) or by using
HMMer with enzyme models (usually from
TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
- ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
- HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.
where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").
Otherwise, a candidate is "medium confidence" if either:
- ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
- ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
- HMMer finds a hit (regardless of coverage or other bits).
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps."
For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways.
For diverse bacteria and archaea that can utilize a carbon source, there is a complete
high-confidence catabolic pathway (including a transporter) just 38% of the time, and
there is a complete medium-confidence pathway 63% of the time.
Gaps may be due to:
- our ignorance of proteins' functions,
- omissions in the gene models,
- frame-shift errors in the genome sequence, or
- the organism lacks the pathway.
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory