Potential Gaps in catabolism of small carbon sources in Mucilaginibacter mallensis MP1X4
Found 95 low-confidence and 45 medium-confidence steps on the best paths for 62 pathways.
| Pathway | Step | Best candidate | 2nd candidate |
|---|
| 4-hydroxybenzoate | mhpD: 2-hydroxypentadienoate hydratase | | |
| 4-hydroxybenzoate | mhpE: 4-hydroxy-2-oxovalerate aldolase | BLU33_RS19350 | |
| 4-hydroxybenzoate | pcaK: 4-hydroxybenzoate transporter pcaK | | |
| 4-hydroxybenzoate | pobA: 4-hydroxybenzoate 3-monooxygenase | | |
| 4-hydroxybenzoate | praA: protocatechuate 2,3-dioxygenase | | |
| 4-hydroxybenzoate | xylF: 2-hydroxymuconate semialdehyde hydrolase | | |
| alanine | cycA: L-alanine symporter CycA | | |
| arginine | rocD: ornithine aminotransferase | BLU33_RS14040 | BLU33_RS14060 |
| arginine | rocE: L-arginine permease | | |
| arginine | rocF: arginase | | |
| cellobiose | glk: glucokinase | BLU33_RS02715 | BLU33_RS17875 |
| citrate | SLC13A5: citrate:Na+ symporter | | |
| citrulline | AO353_03040: ABC transporter for L-Citrulline, ATPase component | BLU33_RS00530 | BLU33_RS19505 |
| citrulline | AO353_03045: ABC transporter for L-Citrulline, permease component 2 | | |
| citrulline | AO353_03050: ABC transporter for L-Citrulline, permease component 1 | | |
| citrulline | AO353_03055: ABC transporter for L-Citrulline, periplasmic substrate-binding component | | |
| citrulline | rocD: ornithine aminotransferase | BLU33_RS14040 | BLU33_RS14060 |
| D-alanine | cycA: D-alanine:H+ symporter CycA | | |
| D-alanine | dadA: D-alanine dehydrogenase | BLU33_RS02880 | |
| D-lactate | D-LDH: D-lactate dehydrogenase | BLU33_RS09740 | BLU33_RS08595 |
| D-lactate | larD: D,L-lactic acid transporter | BLU33_RS15445 | |
| D-serine | cycA: D-serine:H+ symporter CycA | | |
| D-serine | dsdA: D-serine ammonia-lyase | BLU33_RS07380 | BLU33_RS19355 |
| deoxyinosine | deoB: phosphopentomutase | BLU33_RS13775 | |
| deoxyinosine | deoC: deoxyribose-5-phosphate aldolase | | |
| deoxyribonate | deoxyribonate-dehyd: 2-deoxy-D-ribonate 3-dehydrogenase | BLU33_RS04965 | BLU33_RS12805 |
| deoxyribonate | deoxyribonate-transport: 2-deoxy-D-ribonate transporter | | |
| deoxyribonate | garK: glycerate 2-kinase | | |
| deoxyribonate | ketodeoxyribonate-cleavage: 2-deoxy-3-keto-D-ribonate cleavage enzyme | | |
| deoxyribose | deoC: deoxyribose-5-phosphate aldolase | | |
| deoxyribose | deoK: deoxyribokinase | | |
| deoxyribose | deoP: deoxyribose transporter | BLU33_RS11695 | BLU33_RS11040 |
| fucose | fdh: L-fucose 1-dehydrogenase | BLU33_RS04965 | |
| fucose | fucDH: 2-keto-3-deoxy-L-fuconate 4-dehydrogenase | BLU33_RS09730 | BLU33_RS06275 |
| fucose | fuconolactonase: L-fucono-1,5-lactonase | BLU33_RS11025 | |
| galactose | galT: UDP-glucose:alpha-D-galactose-1-phosphate uridylyltransferase | | |
| gluconate | gntK: D-gluconate kinase | BLU33_RS09605 | |
| glucosamine | nagK: N-acetylglucosamine kinase | BLU33_RS18860 | BLU33_RS02715 |
| glucose | glk: glucokinase | BLU33_RS02715 | BLU33_RS17875 |
| glucose-6-P | uhpT: glucose-6-phosphate:phosphate antiporter | | |
| glucuronate | exuT: D-glucuronate:H+ symporter ExuT | | |
| glucuronate | uxuB: D-mannonate dehydrogenase | | |
| glutamate | gltP: L-glutamate:cation symporter GltP/GltT | BLU33_RS16685 | BLU33_RS14195 |
| glycerol | glpD: glycerol 3-phosphate dehydrogenase (monomeric) | BLU33_RS15455 | |
| histidine | hutG: N-formiminoglutamate formiminohydrolase | | |
| histidine | PA5503: L-histidine ABC transporter, ATPase component | BLU33_RS00530 | BLU33_RS09400 |
| histidine | PA5504: L-histidine ABC transporter, permease component | BLU33_RS00535 | BLU33_RS19835 |
| histidine | PA5505: L-histidine ABC transporter, substrate-binding component | BLU33_RS00540 | |
| isoleucine | acdH: (2S)-2-methylbutanoyl-CoA dehydrogenase | BLU33_RS24850 | BLU33_RS17775 |
| isoleucine | Bap2: L-isoleucine permease Bap2 | | |
| isoleucine | bkdA: branched-chain alpha-ketoacid dehydrogenase, E1 component alpha subunit | BLU33_RS24660 | BLU33_RS19455 |
| isoleucine | bkdC: branched-chain alpha-ketoacid dehydrogenase, E2 component | BLU33_RS10760 | BLU33_RS18030 |
| isoleucine | ech: 2-methyl-3-hydroxybutyryl-CoA hydro-lyase | BLU33_RS22635 | BLU33_RS00005 |
| isoleucine | ivdG: 3-hydroxy-2-methylbutyryl-CoA dehydrogenase | BLU33_RS17295 | BLU33_RS10705 |
| isoleucine | pccA: propionyl-CoA carboxylase, alpha subunit | BLU33_RS09750 | BLU33_RS23025 |
| L-lactate | larD: D,L-lactic acid transporter LarD | BLU33_RS15445 | |
| L-lactate | lctO: L-lactate oxidase or 2-monooxygenase | | |
| lactose | glk: glucokinase | BLU33_RS02715 | BLU33_RS17875 |
| lactose | klh: periplasmic 3'-ketolactose hydrolase | | |
| leucine | bkdA: branched-chain alpha-ketoacid dehydrogenase, E1 component alpha subunit | BLU33_RS24660 | BLU33_RS19455 |
| leucine | bkdC: branched-chain alpha-ketoacid dehydrogenase, E2 component | BLU33_RS10760 | BLU33_RS18030 |
| leucine | leuT: L-leucine:Na+ symporter LeuT | | |
| leucine | liuA: isovaleryl-CoA dehydrogenase | BLU33_RS17775 | BLU33_RS24850 |
| leucine | liuB: 3-methylcrotonyl-CoA carboxylase, alpha (biotin-containing) subunit | BLU33_RS09750 | BLU33_RS23025 |
| leucine | liuC: 3-methylglutaconyl-CoA hydratase | BLU33_RS22635 | BLU33_RS04380 |
| leucine | liuD: 3-methylcrotonyl-CoA carboxylase, beta subunit | BLU33_RS08985 | BLU33_RS16640 |
| lysine | hglS: D-2-hydroxyglutarate synthase | | |
| lysine | lysN: 2-aminoadipate transaminase | BLU33_RS14885 | BLU33_RS14060 |
| lysine | lysP: L-lysine:H+ symporter LysP | | |
| lysine | ydiJ: (R)-2-hydroxyglutarate dehydrogenase | BLU33_RS08595 | |
| maltose | glk: glucokinase | BLU33_RS02715 | BLU33_RS17875 |
| mannitol | mt2d: mannitol 2-dehydrogenase | BLU33_RS17295 | BLU33_RS08240 |
| mannitol | PLT5: polyol transporter PLT5 | BLU33_RS04945 | BLU33_RS15240 |
| mannose | manA: mannose-6-phosphate isomerase | BLU33_RS07230 | BLU33_RS05090 |
| mannose | manP: mannose PTS system, EII-CBA components | | |
| myoinositol | iolG: myo-inositol 2-dehydrogenase | | |
| myoinositol | iolM: 2-inosose 4-dehydrogenase | BLU33_RS13085 | BLU33_RS08240 |
| myoinositol | iolN: 2,4-diketo-inositol hydratase | | |
| myoinositol | iolO: 5-dehydro-L-gluconate epimerase | | |
| myoinositol | iolT: myo-inositol:H+ symporter | BLU33_RS04945 | BLU33_RS04860 |
| myoinositol | uxaE: D-tagaturonate epimerase | | |
| myoinositol | uxuB: D-mannonate dehydrogenase | | |
| NAG | nagK: N-acetylglucosamine kinase | BLU33_RS18860 | BLU33_RS02715 |
| phenylacetate | paaA: phenylacetyl-CoA 1,2-epoxidase, subunit A | | |
| phenylacetate | paaB: phenylacetyl-CoA 1,2-epoxidase, subunit B | | |
| phenylacetate | paaC: phenylacetyl-CoA 1,2-epoxidase, subunit C | | |
| phenylacetate | paaE: phenylacetyl-CoA 1,2-epoxidase, subunit E | BLU33_RS17665 | BLU33_RS18820 |
| phenylacetate | paaF: 2,3-dehydroadipyl-CoA hydratase | BLU33_RS22635 | |
| phenylacetate | paaG: 1,2-epoxyphenylacetyl-CoA isomerase / 2-(oxepinyl)acetyl-CoA isomerase / didehydroadipyl-CoA isomerase | BLU33_RS22635 | |
| phenylacetate | paaJ1: 3-oxo-5,6-dehydrosuberyl-CoA thiolase | BLU33_RS24945 | BLU33_RS09250 |
| phenylacetate | paaJ2: 3-oxoadipyl-CoA thiolase | BLU33_RS24945 | BLU33_RS09250 |
| phenylacetate | paaK: phenylacetate-CoA ligase | | |
| phenylacetate | paaT: phenylacetate transporter Paa | | |
| phenylacetate | paaZ1: oxepin-CoA hydrolase | | |
| phenylacetate | paaZ2: 3-oxo-5,6-didehydrosuberyl-CoA semialdehyde dehydrogenase | | |
| phenylalanine | aroP: L-phenylalanine:H+ symporter AroP | | |
| phenylalanine | fahA: fumarylacetoacetate hydrolase | | |
| phenylalanine | maiA: maleylacetoacetate isomerase | | |
| phenylalanine | PAH: phenylalanine 4-monooxygenase | BLU33_RS04185 | |
| phenylalanine | PCBD: pterin-4-alpha-carbinoalamine dehydratase | | |
| phenylalanine | QDPR: 6,7-dihydropteridine reductase | | |
| propionate | pccA: propionyl-CoA carboxylase, alpha subunit | BLU33_RS09750 | BLU33_RS23025 |
| propionate | putP: propionate transporter; proline:Na+ symporter | | |
| putrescine | gabT: gamma-aminobutyrate transaminase | BLU33_RS14040 | BLU33_RS14060 |
| putrescine | patA: putrescine aminotransferase (PatA/SpuC) | BLU33_RS05520 | BLU33_RS14040 |
| putrescine | patD: gamma-aminobutyraldehyde dehydrogenase | BLU33_RS06915 | BLU33_RS14055 |
| putrescine | puuP: putrescine:H+ symporter PuuP/PlaP | | |
| pyruvate | SLC5A8: sodium-coupled pyruvate transporter | | |
| rhamnose | rhaA: L-rhamnose isomerase | | |
| rhamnose | rhaB: L-rhamnulokinase | | |
| rhamnose | rhaD: rhamnulose 1-phosphate aldolase | | |
| rhamnose | rhaM: L-rhamnose mutarotase | BLU33_RS04955 | |
| rhamnose | rhaT: L-rhamnose:H+ symporter RhaT | | |
| ribose | rbsK: ribokinase | | |
| ribose | rbsU: probable D-ribose transporter RbsU | | |
| serine | serP: L-serine permease SerP | | |
| sorbitol | sdh: sorbitol dehydrogenase | BLU33_RS13310 | BLU33_RS08240 |
| sorbitol | SOT: sorbitol:H+ co-transporter SOT1 or SOT2 | BLU33_RS04945 | BLU33_RS15240 |
| threonine | tdcC: L-threonine:H+ symporter TdcC | | |
| thymidine | deoA: thymidine phosphorylase DeoA | BLU33_RS13805 | |
| thymidine | deoB: phosphopentomutase | BLU33_RS13775 | |
| thymidine | deoC: deoxyribose-5-phosphate aldolase | | |
| trehalose | glk: glucokinase | BLU33_RS02715 | BLU33_RS17875 |
| trehalose | klh: 3-ketotrehalose hydrolase | BLU33_RS13315 | BLU33_RS08150 |
| tryptophan | aroP: tryptophan:H+ symporter AroP | | |
| tryptophan | tnaA: tryptophanase | | |
| tyrosine | aroP: L-tyrosine transporter (AroP/FywP) | | |
| tyrosine | fahA: fumarylacetoacetate hydrolase | | |
| tyrosine | maiA: maleylacetoacetate isomerase | | |
| valine | acdH: isobutyryl-CoA dehydrogenase | BLU33_RS24850 | BLU33_RS17775 |
| valine | Bap2: L-valine permease Bap2 | | |
| valine | bch: 3-hydroxyisobutyryl-CoA hydrolase | | |
| valine | bkdA: branched-chain alpha-ketoacid dehydrogenase, E1 component alpha subunit | BLU33_RS24660 | BLU33_RS19455 |
| valine | bkdC: branched-chain alpha-ketoacid dehydrogenase, E2 component | BLU33_RS10760 | BLU33_RS18030 |
| valine | ech: (S)-3-hydroxybutanoyl-CoA hydro-lyase | BLU33_RS22635 | |
| valine | mmsA: methylmalonate-semialdehyde dehydrogenase | BLU33_RS14055 | BLU33_RS06915 |
| valine | mmsB: 3-hydroxyisobutyrate dehydrogenase | BLU33_RS24355 | BLU33_RS13615 |
| valine | pccA: propionyl-CoA carboxylase, alpha subunit | BLU33_RS09750 | BLU33_RS23025 |
| xylitol | PLT5: xylitol:H+ symporter PLT5 | BLU33_RS04945 | BLU33_RS15240 |
| xylitol | xdhA: xylitol dehydrogenase | BLU33_RS08240 | BLU33_RS13085 |
Confidence: high confidence medium confidence low confidence
This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.
Links
Downloads
Related tools
About GapMind
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using
ublast (a fast alternative to protein BLAST)
against a database of manually-curated proteins (most of which are experimentally characterized) or by using
HMMer with enzyme models (usually from
TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
- ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
- HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.
where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").
Otherwise, a candidate is "medium confidence" if either:
- ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
- ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
- HMMer finds a hit (regardless of coverage or other bits).
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps."
For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways.
For diverse bacteria and archaea that can utilize a carbon source, there is a complete
high-confidence catabolic pathway (including a transporter) just 38% of the time, and
there is a complete medium-confidence pathway 63% of the time.
Gaps may be due to:
- our ignorance of proteins' functions,
- omissions in the gene models,
- frame-shift errors in the genome sequence, or
- the organism lacks the pathway.
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory