catabolism of small carbon sources in Spirochaetaceae bacterium RHO1_bin

GapMind for catabolism of small carbon sources

catabolism of small carbon sources in Spirochaetaceae bacterium RHO1_bin_44

Pathways are sorted by name. Sort by completeness instead.

Pathway	Steps
acetate	ybhL, acs
D-alanine	Pf6N2E2_5402, Pf6N2E2_5403, Pf6N2E2_5404, Pf6N2E2_5405, dadA
alanine	cycA
arabinose	araE, araA, araB, araD
arginine	rocE, rocF, odc, patA, patD, gabT, gabD
asparagine	ans, bztA, bztB, bztC, bztD
aspartate	bztA, bztB, bztC, bztD
cellobiose	bglT, bgl, glk
citrate	SLC13A5, acn, icd
citrulline	AO353_03055, AO353_03050, AO353_03045, AO353_03040, arcB, arcC, odc, patA, patD, gabT, gabD
deoxyinosine	nupC, deoD, deoB, deoC, adh, acs
deoxyribonate	deoxyribonate-transport, deoxyribonate-dehyd, ketodeoxyribonate-cleavage, garK, aacS, atoB
deoxyribose	deoP, deoK, deoC, adh, acs
ethanol	etoh-dh-nad, adh, acs
fructose	fruII-ABC, 1pfk, fba, tpi
fucose	fucP, fucU, fdh, fuconolactonase, fucD, fucDH, KDF-hydrolase
fumarate	dctA
galactose	galP, galK, galT, galE, pgmA
galacturonate	exuT, uxaC, uxaB, uxaA, kdgK, eda
gluconate	gntT, gntK, gnd
glucose	MFS-glucose, glk
glucose-6-P	uhpT
glucosamine	gamP, nagB
glucuronate	exuT, udh, gci, garL, garR, garK
glutamate	bztA, bztB, bztC, gltL, gdhA
glycerol	glpF, glpK, glpD, tpi
histidine	aapJ, aapQ, aapM, aapP, hutH, hutU, hutI, hutG
isoleucine	livF, livG, livJ, livH, livM, bkdA, bkdB, bkdC, lpd, acdH, ech, ivdG, fadA, prpC, prpD, acn, prpB
4-hydroxybenzoate	pcaK, pobA, praA, xylF, mhpD, mhpE, adh, acs
D-lactate	lctP, D-LDH
L-lactate	lctP, lctO, acs
lactose	lacP, lacZ, galK, galT, galE, pgmA, glk
leucine	aapJ, aapQ, aapM, aapP, ilvE, bkdA, bkdB, bkdC, lpd, liuA, liuB, liuD, liuC, liuE, aacS, atoB
lysine	lysP, davB, davA, davT, davD, gcdG, gcdH, ech, fadB, atoB
L-malate	sdlC
maltose	malEIICBA, malA, glk
mannitol	PLT5, mt2d, scrK
mannose	manP, manA
myoinositol	PGA1_c07300, PGA1_c07310, PGA1_c07320, iolG, iolE, iolD, iolB, iolC, iolJ, mmsA, tpi
NAG	nagEcba, nagA, nagB
2-oxoglutarate	kgtP
phenylacetate	paaT, paaK, paaA, paaB, paaC, paaE, paaG, paaZ1, paaZ2, paaJ1, paaF, paaH, paaJ2
phenylalanine	aroP, PAH, PCBD, QDPR, HPD, hmgA, maiA, fahA, aacS, atoB
proline	aapJ, aapQ, aapM, aapP, put1, putA
propionate	putP, prpE, prpC, prpD, acn, prpB
putrescine	potA, potB, potC, potD, patA, patD, gabT, gabD
pyruvate	SLC5A8
rhamnose	rhaT, rhaM, rhaA, rhaB, rhaD, tpi, aldA
ribose	rbsU, rbsK
D-serine	cycA, dsdA
serine	serP, sdaB
sorbitol	mtlA, srlD
succinate	sdc
sucrose	ams, fruII-ABC, 1pfk, fba, tpi
threonine	tdcC, ltaE, adh, acs, gcvP, gcvT, gcvH, lpd
thymidine	nupG, deoA, deoB, deoC, adh, acs
trehalose	thuE, thuF, thuG, thuK, treF, glk
tryptophan	aroP, tnaA
tyrosine	aroP, HPD, hmgA, maiA, fahA, aacS, atoB
valine	livF, livG, livJ, livH, livM, bkdA, bkdB, bkdC, lpd, acdH, ech, bch, mmsB, mmsA, prpC, prpD, acn, prpB
xylitol	PLT5, xdhA, xylB
xylose	xylT, xylA, xylB

Confidence: high confidence medium confidence low confidence
transporter – transporters and PTS systems are shaded because predicting their specificity is particularly challenging.

This GapMind analysis is from Jul 26 2024. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the 2024 update on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources