Alignments for a candidate for xad in Azospirillum brasilense Sp245

GapMind for catabolism of small carbon sources

Alignments for a candidate for xad in Azospirillum brasilense Sp245

Align xylonate dehydratase (EC 4.2.1.82) (characterized)
to candidate AZOBR_RS15015 AZOBR_RS15015 dihydroxy-acid dehydratase

Query= BRENDA::Q9A9Z2
         (595 letters)



>FitnessBrowser__azobra:AZOBR_RS15015
          Length = 621

 Score =  185 bits (470), Expect = 4e-51
 Identities = 181/601 (30%), Positives = 266/601 (44%), Gaps = 97/601 (16%)

Query: 48  GKPIIGIAQTGSDISPCNRIHL-DLVQRVRDGIRDAGGIPMEFPVHPIFENCRRPTAALD 106
           GKPII IA + +   P   +HL DL Q V   I  AGG+  EF    + +        + 
Sbjct: 33  GKPIIAIANSFTQFVP-GHVHLKDLGQLVAREIEKAGGVAKEFNTIAVDDGIAMGHDGML 91

Query: 107 RNLSYLGLVETLHGYPI-----DAVVLTTGCDKTTPAGIMAATTVNIPAIVLSGGPMLDG 161
            +L    L+     Y +     DA+V  + CDK TP  +MAA  +NIPA+ +SGGPM   
Sbjct: 92  YSLPSRELIADAVEYMVNAHCADALVCISNCDKITPGMLMAAMRLNIPAVFVSGGPM--- 148

Query: 162 WHENELVGSGTVIWRSRRK--------LAAGE--ITEEEFIDRAASSAPSAGHCNTMGTA 211
                   +G V WR + K        +AA +  +++EE       S P+ G C+ M TA
Sbjct: 149 -------EAGKVNWRGKTKAVDLIDAMVAAADPTVSDEEAAVMERGSCPTCGSCSGMFTA 201

Query: 212 STMNAVAEALGLSLTGCAAIPAPYRERGQMAYKTGQRIVDLA---YDD----VKPLDILT 264
           ++MN + EALGLSL G   I A + +R ++    G+  V+L    Y +      P  I T
Sbjct: 202 NSMNCLTEALGLSLPGNGTILATHADRKELFLAAGRMAVELCRRWYQEEDATALPRGIAT 261

Query: 265 KQAFENAIALVAAAGGSTNAQPHIVAMARHAGVEIT-ADDWRAAYDIPLIVNMQPAGKYL 323
            +AFENA+ L  A GGSTN   H++A A+   V  T AD  R +  +P +  + PA   +
Sbjct: 262 FEAFENAMTLDIAMGGSTNTVLHLLAAAQEGQVPFTMADIDRLSRRVPNVCKVAPAVSDV 321

Query: 324 G-ERFHRAGGAPAVLWELLQQGRLHGDVLTVTGKTMSENL----------QGRET----- 367
             E  H+AGG   +L EL + G L+ DV TV  KT+ + L          +G  T     
Sbjct: 322 HIEDVHKAGGIFGILGELDRGGLLNRDVATVHAKTLGDALDRWDVKRTQDEGVHTMFKAA 381

Query: 368 ----------------------SDREVIFPYHEPLAEKAGFLVLKGNLFDFAIMKSSVIG 405
                                  D+ VI       ++  G  VL GN+ +    K  ++ 
Sbjct: 382 PGGIPTTIAFSQEKRWPELDLDRDKGVIRSVDSAFSKDGGLAVLFGNIAE----KGCIV- 436

Query: 406 EEFRKRYLSQPGQEGVFEARAIVFDGSDDYHKRINDPALEIDERCILVIRGAGPIGWPGS 465
                +         VF   A VF+  D   + I    ++  +  ++VIR  GP G PG 
Sbjct: 437 -----KTAGVDASNLVFAGPARVFESQDAAVEAILGDTVKAGD--VVVIRYEGPRGGPGM 489

Query: 466 AEVVNMQPPDHLLKKGI-MSLPTLGDGRQSGTADSPSILNASPESAIGGGLSWLRTGDTI 524
            E+  + P  +L  KG+  +   + DGR SG     SI +ASPE+A GG +  ++ GD I
Sbjct: 490 QEM--LYPTSYLKSKGLGKACALVTDGRFSGGTSGLSIGHASPEAAQGGAIGLVQDGDRI 547

Query: 525 RIDLNTGRCD-ALVDEATIAARKQDGIPAVPA--------TMTPWQEIYRAHASQLDTGG 575
            ID+   + + AL DE     R  +      A         ++P    Y A  +  D G 
Sbjct: 548 EIDIPNRKINLALSDEELQRRRDAENAKGADAWKPANRNRVVSPALRAYAALTTSADRGA 607

Query: 576 V 576
           V
Sbjct: 608 V 608


Lambda     K      H
   0.319    0.136    0.411 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 924
Number of extensions: 52
Number of successful extensions: 7
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 2
Number of HSP's successfully gapped: 2
Length of query: 595
Length of database: 621
Length adjustment: 37
Effective length of query: 558
Effective length of database: 584
Effective search space:   325872
Effective search space used:   325872
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 53 (25.0 bits)

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Sep 17 2021.

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Protein sequences (fasta format)
Organisms (tab-delimited)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources