Alignments for a candidate for drdehyd-beta in Paraburkholderia bryophila 376MFSha3.1

GapMind for catabolism of small carbon sources

Alignments for a candidate for drdehyd-beta in Paraburkholderia bryophila 376MFSha3.1

Align 2-deoxy-D-ribose dehydrogenase β subunit (characterized)
to candidate H281DRAFT_05400 H281DRAFT_05400 isoquinoline 1-oxidoreductase, beta subunit

Query= metacyc::MONOMER-20833
         (739 letters)



>FitnessBrowser__Burk376:H281DRAFT_05400
          Length = 749

 Score =  413 bits (1062), Expect = e-119
 Identities = 261/747 (34%), Positives = 401/747 (53%), Gaps = 55/747 (7%)

Query: 14  LSRRRFL------ASTAVGALVIGFGLPLGAGRVQAATS---------AERGTQVP-AFL 57
           +SRR FL       + A G L++GF +P  +   +A  S         A+ G   P AF+
Sbjct: 24  VSRRTFLKLSVTVGAAAGGGLLLGFSMPAVSQDQKAGKSVIGGDANEAAQNGVFAPNAFI 83

Query: 58  EIRPDGTVRLLSPFMEGGQGTHTAMAQIVGEELDADPATFIVEAAPPGEA-YVVMENGLR 116
           +I   G V L+ P +E GQG +T++  ++ EEL+    T  ++ APP E  +     G +
Sbjct: 84  QIDTAGKVTLVIPKVEMGQGVYTSIPMLIAEELEVPLDTVTLDHAPPDEKLFTDPLLGGQ 143

Query: 117 ITGGSMSVRMSYPTMRRLGALARAMLLQAGAKQWGVPVGELTTQPGRVVHAASGRSLGYG 176
           +TGGS S+R ++  MR+ GA AR +L+ A A+QW V       + G+V+HAAS RS+GYG
Sbjct: 144 LTGGSTSIRYAWEPMRKAGATARMLLIGAAAQQWQVDASSCHAKSGQVIHAASNRSIGYG 203

Query: 177 ELASSALDMPVPDPASITLRDPSQFRWIGKPVKRLDAYDKSTGKALYSIDLKVDNMLHAA 236
           +L  +A  +PVP   ++ L+DP  F+ IG  VKRLD+ +K  G A + +D+++ +M++AA
Sbjct: 204 QLVDAAAKLPVPQ--NVPLKDPKDFKIIGTAVKRLDSPEKVDGTATFGLDVRLPDMVYAA 261

Query: 237 VQHAPRLGMTVGSLRNQSQVEGMKGVHSVHVLPGAVAVVAERWWHAKRAVEAIQVDWQEA 296
           + + P  G  + S+ + +  + + GV  V  +  AVAV+ +  W AKR ++A+ + W E 
Sbjct: 262 IANCPVFGGKLASV-DDTNAKKIPGVRQVVKIDNAVAVIGDHTWAAKRGLQALDIKWNEG 320

Query: 297 A-ADSALRVMPADFSSDKHREFLAAQQGPTRDDENEGDVAGALKGAKTQVEATYHNQYLN 355
           A A  +++ +  D ++   R    A++        +GDV  A   AKT+V+A Y   +L 
Sbjct: 321 ADAKLSMKQIVDDLANASQRNGAVARK--------DGDVVHAFSNAKTRVDAVYQQPFLA 372

Query: 356 HAQLEPPSALARFNPDGTLEIWLPNQAPDMFRADIAKRTGLDPAQITLHSPLLGGFFGRH 415
           HA +EP +      PDG  EIWL +Q P   R      TGL   +I +H+ L+GG FGR 
Sbjct: 373 HATMEPINCTVHVRPDGC-EIWLGSQVPTRVRDAAMAVTGLPADKIVVHNHLIGGGFGRR 431

Query: 416 FLYDSANPYPQAIALAKAVSRPIKLIWSREEEFLRDVLRPLAVVKFRAALDDKGLPVAIE 475
             +D      QA+ + K VS P+K++W+REE+   D+ RP    K  A LD  G P+A +
Sbjct: 432 LEFDMVT---QAVKIGKQVSTPVKVLWTREEDIQHDMYRPYYYDKISAGLDANGKPLAWQ 488

Query: 476 ------AVSATEGPTEALAGKQGDKIDPTAVEGLSGKSYAIPNKRIAQIYVKGAPML--- 526
                 ++ A   P    A K G  +DP AVE  S   Y +PN+ +   YV+  P     
Sbjct: 489 HRIVGSSIMARFAPP---AFKDG--LDPDAVEVASDLPYDLPNQLVD--YVRQEPHAIPT 541

Query: 527 GYWRSVGNSLNDFFYESFLDELADKGGHDPYELRLHLLRDNPRLTTLLQAAGELSGGWKR 586
            +WR VG +   F  ESF+DELA +   DP + R  LL   PR   +L  A + +     
Sbjct: 542 AFWRGVGPTRGTFVVESFIDELAAEAKVDPVKYRRDLLGKTPRALNVLNTAAQAANWGSA 601

Query: 587 GPFTAEDGTRRARGVAMASPFGSHTAAIAEVSIENGKVKVHDIWQAIDPGSIVNPAIVEA 646
            P        + RGV++   FGS+ A + +V+++ G+V V  +  A+D G +VNP  VEA
Sbjct: 602 VP------KGQGRGVSVMHAFGSYFAIVVDVAVDQGEVAVKRVVCAVDCGMVVNPNTVEA 655

Query: 647 QVNGAVALGLSQTLLEEAVYVDGKPRARNYDLYPILAPAQMARVHVRVVESGEKMGGIGE 706
           QV G +  G++  L  E    DG+    N+  Y +L   +   + V +V+S E  GGIGE
Sbjct: 656 QVQGGIIFGITAALYSEITIKDGRVEQNNFTDYRMLRIDETPPIDVHIVKSSEAPGGIGE 715

Query: 707 PPLPAVAPAVANAVAQLTGQRVRSLPL 733
           P   A+APA+ NA+   TG+R+R LP+
Sbjct: 716 PGTAALAPALTNAIFAATGKRLRQLPV 742


Lambda     K      H
   0.317    0.134    0.394 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1341
Number of extensions: 71
Number of successful extensions: 10
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 739
Length of database: 749
Length adjustment: 40
Effective length of query: 699
Effective length of database: 709
Effective search space:   495591
Effective search space used:   495591
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 55 (25.8 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources