Align Maltose transport system permease protein malG aka TT_C1629, component of The trehalose/maltose/sucrose/palatinose porter (TTC1627-9) plus MalK1 (ABC protein, shared with 3.A.1.1.24) (Silva et al. 2005; Chevance et al., 2006). The receptor (TTC1627) binds disaccharide alpha-glycosides, namely trehalose (alpha-1,1), sucrose (alpha-1,2), maltose (alpha-1,4), palatinose (alpha-1,6) and glucose (characterized)
to candidate N515DRAFT_3133 N515DRAFT_3133 carbohydrate ABC transporter membrane protein 2, CUT1 family (TC 3.A.1.1.-)
Query= TCDB::Q72H66 (280 letters) >FitnessBrowser__Dyella79:N515DRAFT_3133 Length = 273 Score = 151 bits (382), Expect = 1e-41 Identities = 92/266 (34%), Positives = 148/266 (55%), Gaps = 6/266 (2%) Query: 16 LVVFVVVYSVFPFYWAVISSF-KPSDALFSPDPSFLPVPFTLEHYENVFLQANFGRNLLN 74 L++ + +VFP W + SF +P +A P P LP TL +Y +F +A GR LLN Sbjct: 13 LLIGSTLVAVFPLLWMLSVSFMRPGEASALPPP-LLPTHATLANYHELFERAGMGRYLLN 71 Query: 75 SLIVAGGATLLSLVLGVLAAYALGRLPFPPKNAVMYIVLSMTMFPQIAVLGGLFLLLRQT 134 SL V+ TLLSL ++A YA +L F + + ++L + P + LFLLL+ Sbjct: 72 SLGVSSAITLLSLAFNLMAGYAFAKLRFSGRERLFQVLLGGLVIPAQVAMLPLFLLLKYL 131 Query: 135 GLFNTHLGLILTYLLFTLPFTVWVLVGYFRGLPRELEEAAYVDGATPLQTLLKVMLPLTG 194 GL N++ +++ + F ++++ Y RG+P +L EAA +DGA L+ ++++LPL Sbjct: 132 GLVNSYAAVVVPAM--ATIFGIFLVRQYARGIPDDLMEAARIDGAGELRIFVQIVLPLLK 189 Query: 195 PGLVTTGLLAFIAAWNEYLFALTFTVGDSVKTVPPAIASFGGATPFEIPWGSIMAASVVV 254 P +VT + F+ AWN++++ L G T+P A+AS + +MA SVV Sbjct: 190 PIMVTLAIFTFLTAWNDFMWPLIALTGQEHYTLPIALASLSREHVQDSE--LMMAGSVVT 247 Query: 255 TVPLVVLVLVFQQRIVAGLTAGAVKG 280 +P++VL L Q+ + GL G+VKG Sbjct: 248 VLPVLVLFLALQRYYLQGLLLGSVKG 273 Lambda K H 0.329 0.145 0.439 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 141 Number of extensions: 6 Number of successful extensions: 2 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 280 Length of database: 273 Length adjustment: 25 Effective length of query: 255 Effective length of database: 248 Effective search space: 63240 Effective search space used: 63240 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 47 (22.7 bits)
This GapMind analysis is from Apr 09 2024. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory