Align monosaccharide-transporting ATPase (EC 3.6.3.17) (characterized)
to candidate Synpcc7942_1680 Synpcc7942_1680 Sulphate transport system permease protein 1
Query= BRENDA::Q97UY8 (353 letters) >FitnessBrowser__SynE:Synpcc7942_1680 Length = 338 Score = 196 bits (498), Expect = 7e-55 Identities = 113/289 (39%), Positives = 178/289 (61%), Gaps = 15/289 (5%) Query: 2 VRIIVKNVSKVFKKGKVVALDNVNINIENGERFGILGPSGAGKTTFMRIIAGLDVPSTGE 61 V I V VSK F G A+ +V++ +E G +LGPSG+GK+T +R+IAGL+ P +G Sbjct: 1 VGIQVSQVSKQF--GSFQAVKDVDLTVETGSLVALLGPSGSGKSTLLRLIAGLEQPDSGR 58 Query: 62 LYFDDRLVASNGKLIVPPEDRKIGMVFQTWALYPNLTAFENIAFPLTNMKMSKEEIRKRV 121 ++ R A+N + DR+IG VFQ +AL+ +LT +NIAF L K +KE++R RV Sbjct: 59 IFLTGR-DATNESV----RDRQIGFVFQHYALFKHLTVRKNIAFGLELRKHTKEKVRARV 113 Query: 122 EEVAKILDIHHVLNHFPRELSGGQQQRVALARALVKDPSLLLLDEPFSNLDARMRDSARA 181 EE+ +++ + + + +P +LSGGQ+QRVALARAL P +LLLDEPF LDA++R R+ Sbjct: 114 EELLELVQLTGLGDRYPSQLSGGQRQRVALARALAVQPQVLLLDEPFGALDAKVRKDLRS 173 Query: 182 LVKEVQSRLGVTLLVVSHDPADIFAIADRVGVLVKGKLVQVGKPEDLYDNPVSIQVASLI 241 ++++ + VT + V+HD + +AD++ V+ GK+ Q+G P ++YDNP + V S I Sbjct: 174 WLRKLHDEVHVTTVFVTHDQEEAMEVADQIVVMNHGKVEQIGSPAEIYDNPATPFVMSFI 233 Query: 242 GEINELEGKVTNEGVVIGSLRFPVSVSSDRAIIGIRPEDVKLSKDVIKD 290 G +N L ++ G L P + +RP D++++ D I + Sbjct: 234 GPVNVLPN--SSHIFQAGGLDTP------HPEVFLRPHDIEIAIDPIPE 274 Lambda K H 0.319 0.139 0.390 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 338 Number of extensions: 15 Number of successful extensions: 2 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 353 Length of database: 338 Length adjustment: 29 Effective length of query: 324 Effective length of database: 309 Effective search space: 100116 Effective search space used: 100116 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 49 (23.5 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory