Align Methylcrotonoyl-CoA carboxylase (EC 6.4.1.4) (characterized)
to candidate WP_002719660.1 RSPH17029_RS05945 methylcrotonoyl-CoA carboxylase
Query= reanno::Smeli:SM_b21122 (535 letters) >NCBI__GCF_000015985.1:WP_002719660.1 Length = 534 Score = 832 bits (2149), Expect = 0.0 Identities = 409/532 (76%), Positives = 462/532 (86%) Query: 4 LRSHISPSSEEFKANRAAMTEAIATIEDAVRLAAAGGGETARERHVSRGKLLPRDRLATL 63 L S + PSSE F+ANRA IA +A AAAGGG A+ RH +RGKL PR+R+A L Sbjct: 3 LSSRVLPSSETFRANRAHHLALIAETAEAAAWAAAGGGPAAQARHAARGKLPPRERVAGL 62 Query: 64 IDPGTPFLEVGATAAYGMYNDDAPGAGLITGIGRISARECMIVCNDPTVKGGTYYPLTVK 123 +DPG+PFLE+GA AA+G+Y+ AP AG+I GIGR+ +E M+VCND TVKGGTYYP+TVK Sbjct: 63 LDPGSPFLEIGAFAAHGLYDGAAPAAGVIAGIGRVHGQEVMVVCNDATVKGGTYYPMTVK 122 Query: 124 KHLRAQEIAAENRLPCVYLVDSGGANLPNQDEVFPDRDHFGRIFYNQANMSAAGIPQIAV 183 KHLRAQEIAAE RLPCVYLVDSGGANLPNQDEVFPDRDHFGRIFYNQA MSAAGIPQIAV Sbjct: 123 KHLRAQEIAAECRLPCVYLVDSGGANLPNQDEVFPDRDHFGRIFYNQAQMSAAGIPQIAV 182 Query: 184 VMGSCTAGGAYVPAMSDEAIIVEKQGTIFLAGPPLVRAATGEVVSAEDLGGADVHTRLSG 243 VMGSCTAGGAYVPAMSD IIV QGTIFLAGPPLV+AATGEVVSAEDLGG +VHTRLSG Sbjct: 183 VMGSCTAGGAYVPAMSDVTIIVRNQGTIFLAGPPLVKAATGEVVSAEDLGGGEVHTRLSG 242 Query: 244 VADHLARDDAHALALARRAVSALNREKPWTVERIEPEPPLYDPEEIAGIVPADLKTPYEI 303 VAD+LA DDAHALALARRA+ LNR +P TV PE P +DPEEI G+VPADL+TP++I Sbjct: 243 VADYLAEDDAHALALARRALGQLNRTRPETVAWAAPEDPAHDPEEILGLVPADLRTPWDI 302 Query: 304 REVIARLVDGSRFDEFKARFGTTLVCGFAHVHGIPVGIVANNGVLFSESAVKGAHFVELC 363 RE+IARLVDGSRFDEFK RFG TLV GFAH+ G PVGIVANNGVLFSE+A+KGAHFVELC Sbjct: 303 RELIARLVDGSRFDEFKPRFGETLVTGFAHLMGCPVGIVANNGVLFSEAAIKGAHFVELC 362 Query: 364 AQRRIPLVFLQNITGFMVGRKYETEGIAKHGAKLVTAVATVKVPKITMLVGGSFGAGNYG 423 +QR IPL+FLQNITGFMVGR+YE EGIA+HGAK+VTAVAT VPKIT+LVGGSFGAGNYG Sbjct: 363 SQRSIPLIFLQNITGFMVGRRYENEGIARHGAKMVTAVATTAVPKITLLVGGSFGAGNYG 422 Query: 424 MCGRAFSPRFLWTWPNSRISVMGGEQAAGVLSSVRGEALKRSGKPWSEEEEARFRQPVLD 483 M GRA+SPRFLWTWPNSRISVMGGEQAAGVL++VR +A++R G WS EEEA F++P ++ Sbjct: 423 MAGRAYSPRFLWTWPNSRISVMGGEQAAGVLATVRRDAIERQGGHWSAEEEAAFKRPTIE 482 Query: 484 LFERQSHPLYASARLWDDGVIDPRKSRDVLALSLSAALNAPIEETRFGLFRM 535 +FERQSHPL+ASARLWDDG++DPRKSR+ L LSLSAALNAPI TRFGLFRM Sbjct: 483 MFERQSHPLHASARLWDDGIVDPRKSRETLFLSLSAALNAPIAPTRFGLFRM 534 Lambda K H 0.320 0.136 0.404 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 917 Number of extensions: 36 Number of successful extensions: 1 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 535 Length of database: 534 Length adjustment: 35 Effective length of query: 500 Effective length of database: 499 Effective search space: 249500 Effective search space used: 249500 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 52 (24.6 bits)
This GapMind analysis is from Apr 10 2024. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory