Alignments for a candidate for mcm-large in Thauera aminoaromatica S2

GapMind for catabolism of small carbon sources

Alignments for a candidate for mcm-large in Thauera aminoaromatica S2

Align methylmalonyl-CoA mutase (subunit 2/2) (EC 5.4.99.2) (characterized)
to candidate WP_004298371.1 C665_RS01390 methylmalonyl-CoA mutase

Query= BRENDA::O74009
         (563 letters)



>NCBI__GCF_000310185.1:WP_004298371.1
          Length = 1088

 Score =  304 bits (778), Expect = 1e-86
 Identities = 209/587 (35%), Positives = 320/587 (54%), Gaps = 60/587 (10%)

Query: 25   KKFLEKAPERKEKFMTDD--------------------GFEIKRIYTP--ADLGEDWNYM 62
            KK L+  P+ +E +  D+                    G +I+++  P   D GE   ++
Sbjct: 506  KKLLDMWPKTRELYAADEYVVKIRDKEIRTQLTSTSLSGSKIRKVALPNFEDAGETLKFL 565

Query: 63   EKLGFPGEYPFTRGVYATMYRGRIWTMRQYAGYATAEESNKRYKYLLSQGQTG--LSVAF 120
             K   PG +P+T GV+A    G   T R +AG   A  +N+R+K + S+G     LS AF
Sbjct: 566  MKENVPGSFPYTAGVFAFKREGEDPT-RMFAGEGDAFRTNRRFKKV-SEGMPAHRLSTAF 623

Query: 121  DLPTQLGYDSD-HPLAEGEVGKVGVAIDSLWDMRILFDGIPL--DKVSTSMTINSTAANL 177
            D  T  G D D  P   G++G  GV+I +L DM++L+DG  L     S SMTIN  A  +
Sbjct: 624  DSVTLYGCDPDLRPDIYGKIGNSGVSIATLDDMKVLYDGFDLCCPTTSVSMTINGPAPII 683

Query: 178  LAMYILVAEEQGVSQEK----------------------LRGTVQNDILKEYIARGTYIF 215
            LA +   A +Q V++ K                      +RGTVQ DILKE   + T IF
Sbjct: 684  LAFFFNTALDQQVTKFKADNGREPTEDELQKIKEWTLSTVRGTVQADILKEDQGQNTCIF 743

Query: 216  PPQPSMRLTTDIIMYCAEN-VPKWNPISISGYHIREAGANAVQEVAFTLADGIEYVKAVI 274
              + ++++  DI  Y   + V  +  +SISGYHI EAGAN + ++AFTL++G  YV++ +
Sbjct: 744  STEFALKMMGDIQEYFVHHDVKNFYSVSISGYHIAEAGANPISQLAFTLSNGFTYVESYL 803

Query: 275  ERGMDVDKFAPRLSFFFAAHNNFLEEIAKF-RAARRLWAYIMKEWFNAKNPRSMMLRFHT 333
             RGM +D FAP LSFFF+  N    E +   R ARR+WA  MK  + A N RS  L++H 
Sbjct: 804  ARGMHIDDFAPNLSFFFS--NGMDPEYSVIGRVARRIWAVAMKNKYGA-NERSQKLKYHV 860

Query: 334  QTAGSTLTAQQPENNIVRVAIQALAAVLGGTQSLHTNSYDEALSLPTEKSVRIALRTQQI 393
            QT+G +L AQ+ + N +R  +QAL A+     SLHTN+YDEA++ PTE+SVR A+  Q I
Sbjct: 861  QTSGRSLHAQEMDFNDIRTTLQALIAIYDNCNSLHTNAYDEAITTPTEESVRRAMAIQLI 920

Query: 394  IAYESGVVDTVDPLGGAYYIEWLTDHIYEEALKYIEKIQKMGGMMRAIERGYVQKEIAEA 453
            I  E G+    +P  GA+ IE LTD + E  LK  E I   GG++ A+E GY + +I E 
Sbjct: 921  INREWGLAKNENPNQGAFIIEELTDLVEEAVLKEFEAIASRGGVLGAMETGYQRGKIQEE 980

Query: 454  AYKYQKEIEEGKRIIVGVNAFVTDEPIEVEILKVDPSIREKQIERLKKLRS--ERDNKKV 511
            +  Y+ +  +G   I+GVN F+  +    + +++  S  E++  +LK+L     R+  + 
Sbjct: 981  SLYYEHKKHDGSYPIIGVNTFLNPKGQAQQEIELARSTEEEKQGQLKRLADFHARNKDQA 1040

Query: 512  QEALDKLRNAAEKEDENLMPYIIEAHRHLATLQEVTDVLREIWGEYR 558
             +   KL+ A   ++ N+   +++A R+  +L ++T+ L ++ G+YR
Sbjct: 1041 PKWQAKLQQAV-IDNTNVFEVLVDAVRY-CSLGQITEALYKVGGQYR 1085


Lambda     K      H
   0.318    0.134    0.388 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1225
Number of extensions: 58
Number of successful extensions: 6
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 563
Length of database: 1088
Length adjustment: 41
Effective length of query: 522
Effective length of database: 1047
Effective search space:   546534
Effective search space used:   546534
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 55 (25.8 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources