Align 3-hydroxybutyryl-CoA dehydrogenase (EC 1.1.1.157); 3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35); short-chain-enoyl-CoA hydratase (EC 4.2.1.150) (characterized)
to candidate WP_004300927.1 C665_RS04215 NAD-binding 3-hydroxyacyl-CoA dehydrogenase
Query= BRENDA::A4YDS4 (651 letters) >NCBI__GCF_000310185.1:WP_004300927.1 Length = 671 Score = 191 bits (486), Expect = 7e-53 Identities = 132/346 (38%), Positives = 187/346 (54%), Gaps = 26/346 (7%) Query: 3 VTVIGSGVMGHGIAELAAIAGNEVWMNDISTEILQQAMERIKWSLSKLRESGSLKEGVEQ 62 V V+G+GVMG IA + A+ G V + D S E + A+ R L + R G ++ V Sbjct: 315 VHVVGAGVMGGDIAAVCALRGMTVTLQDQSVERIAPAIARAA-KLFERRHKGDARQ-VRF 372 Query: 63 VLARIHPETDQAQALKGSDFVIEAVKEDLELKRTIFRNAEAHASPSAVLATNTSSLPISE 122 L R+ P+ A + +D +IEA+ E+LE+KR++F EA A P AVLATNTSSL + + Sbjct: 373 TLDRLIPDPKGEGAAR-ADVIIEAIFENLEVKRSLFAELEARAKPDAVLATNTSSLRLED 431 Query: 123 IASVLKSPQRVVGMHFFNPPVLMPLVEIVRGKDTSDEVVKTTAEMAKSMNKETIVVKDVP 182 IA+ LK P R+VG+HFFNP ++PLVE+V+G+ T EV++ + ++K + VK P Sbjct: 432 IATALKDPSRLVGIHFFNPVPMLPLVEVVQGEATDAEVLRRATGFVRRIDKLPLPVKSAP 491 Query: 183 GFFVNRVLLRIMEAGCYLVEKGIASIQEVDSSAIEELGFPMGVFLLADYTGLDIGYSVWK 242 GF VN VL M VE+G+A E+ +A+ G PMG L D GLDI + K Sbjct: 492 GFLVNSVLGPYMLEALRCVEEGVA--PEMVDAALVAFGMPMGPVELVDTVGLDIAVAAGK 549 Query: 243 AVTARGFKAFPCSSTEKLVSQGKLGVKSGSGYYQYPSPGKFV----------------RP 286 A+ G A P + KLV +GKLG KSG GYY++ GK V RP Sbjct: 550 ALAGEG--AEPPARLLKLVGEGKLGRKSGEGYYRWVD-GKAVKAGDEDERKGGLWRKRRP 606 Query: 287 T-LPSTSKKLGRYLISPAVNEVSYLLREGIVGKDD-AEKGCVLGLG 330 P L +++P + + G+VG D A+ G + G G Sbjct: 607 APPPPIPAGLAERVLAPLLAATRRCVELGVVGDADLADAGVIFGTG 652 Score = 102 bits (255), Expect = 4e-26 Identities = 70/212 (33%), Positives = 112/212 (52%), Gaps = 7/212 (3%) Query: 402 RVEPPLAWIVLNRP-TRYNAINGDMIREINQALDSLEEREDVRVIAITGQGRVFSAGADV 460 R E LAW+ L++ N ++ D++ E LD L+ ++ + + F AGAD+ Sbjct: 16 REETGLAWLDLDKAEASANVLSADVLAEFAGVLDVLDAVPPKALVIASAKPAGFIAGADI 75 Query: 461 TEFGSLTPVKA--MIASRKFHEVFMKIQFLTKPVIAVINGLALGGGMELALSADFRVA-- 516 EF L V A M+ R + +F ++ ++ P +A+I G +GGG+ELAL+ R+ Sbjct: 76 EEFSRLDSVLAARMLVERGWL-LFNRLAAVSYPTLALIRGHCMGGGLELALACRHRIVVD 134 Query: 517 SKTAEMGQPEINLGLIPGGGGTQRLSRLSG-RKGLELVLTGRRVKAEEAYRLGIVEFLAE 575 ++ PE+ LG++PG GG RL L G L+L+LTG+ V A++A RLG+ + Sbjct: 135 EPGTKLALPEVMLGIVPGWGGMLRLPALIGPAAALDLMLTGKSVDAKKAKRLGLADDCVP 194 Query: 576 PEELESEVRKLANAIAEKSPLAVASAKLAYKL 607 P +ES R LA + K PL L + + Sbjct: 195 PRVMESAARILALSGVAKKPLPFKERALRWAM 226 Score = 45.8 bits (107), Expect = 6e-09 Identities = 26/96 (27%), Positives = 47/96 (48%), Gaps = 6/96 (6%) Query: 299 LISPAVNEVSYLLREGIVGKDDAEKGCVLGLGLPKGILSYADEIGIDVVVNTLEEMRQTS 358 ++ P + E + EG+ + ++ G+P G + D +G+D+ V + + Sbjct: 498 VLGPYMLEALRCVEEGVA--PEMVDAALVAFGMPMGPVELVDTVGLDIAVAAGKALAGEG 555 Query: 359 GMDHYSPDPLLLSMVKEGKLGRKSGQGFHTYAHEEA 394 P LL +V EGKLGRKSG+G++ + +A Sbjct: 556 A----EPPARLLKLVGEGKLGRKSGEGYYRWVDGKA 587 Lambda K H 0.316 0.134 0.377 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 925 Number of extensions: 45 Number of successful extensions: 9 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 3 Number of HSP's successfully gapped: 3 Length of query: 651 Length of database: 671 Length adjustment: 38 Effective length of query: 613 Effective length of database: 633 Effective search space: 388029 Effective search space used: 388029 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 54 (25.4 bits)
This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory