Align Alpha-ketoglutaric semialdehyde dehydrogenase 1; alphaKGSA dehydrogenase 1; 2,5-dioxovalerate dehydrogenase 1; 2-oxoglutarate semialdehyde dehydrogenase 1; KGSADH-I; Succinate-semialdehyde dehydrogenase [NAD(+)]; SSDH; EC 1.2.1.26; EC 1.2.1.24 (characterized)
to candidate WP_004310529.1 C665_RS11695 NAD-dependent succinate-semialdehyde dehydrogenase
Query= SwissProt::Q1JUP4 (481 letters) >NCBI__GCF_000310185.1:WP_004310529.1 Length = 478 Score = 706 bits (1823), Expect = 0.0 Identities = 349/478 (73%), Positives = 401/478 (83%) Query: 4 VTYTDTQLLIDGEWVDAASGKTIDVVNPATGKPIGRVAHAGIADLDRALAAAQSGFEAWR 63 +TY T+LLIDG+W DA G+T+ V NPATG+ IGRVAHA IADLD A+AAA GF+ WR Sbjct: 1 MTYPHTRLLIDGQWCDALDGRTLAVHNPATGEEIGRVAHAAIADLDLAVAAAVKGFQTWR 60 Query: 64 KVPAHERAATMRKAAALVRERADAIAQLMTQEQGKPLTEARVEVLSAADIIEWFADEGRR 123 + PA ERA T+R+AAAL+RERA IA+++TQEQGKPL EA++E L+AADIIEWFADEG R Sbjct: 61 RTPAIERAKTLRRAAALMRERAGDIARVLTQEQGKPLPEAKMETLAAADIIEWFADEGLR 120 Query: 124 VYGRIVPPRNLGAQQTVVKEPVGPVAAFTPWNFPVNQVVRKLSAALATGCSFLVKAPEET 183 VYGRIVP RNL A Q V+K+PVGPVAAFTPWNFP+NQVVRK +AALATGCS LVKA EET Sbjct: 121 VYGRIVPGRNLAATQMVIKDPVGPVAAFTPWNFPINQVVRKAAAALATGCSILVKAAEET 180 Query: 184 PASPAALLRAFVDAGVPAGVIGLVYGDPAEISSYLIPHPVIRKVTFTGSTPVGKQLASLA 243 PA+PA L+RAFVDAGVPAGVIGLVYG+PAEISSYLI HP IRK+TFTGSTPVGKQLA+LA Sbjct: 181 PAAPAELVRAFVDAGVPAGVIGLVYGNPAEISSYLIAHPAIRKITFTGSTPVGKQLAALA 240 Query: 244 GLHMKRATMELGGHAPVIVAEDADVALAVKAAGGAKFRNAGQVCISPTRFLVHNSIRDEF 303 G HMKR TMELGGHAPVIV EDAD+ LA+K + +KFRNAGQVCISPTRFLVH ++R+ F Sbjct: 241 GQHMKRVTMELGGHAPVIVCEDADLELAIKVSAASKFRNAGQVCISPTRFLVHEAVRETF 300 Query: 304 TRALVKHAEGLKVGNGLEEGTTLGALANPRRLTAMASVIDNARKVGASIETGGERIGSEG 363 AL +HA LKVG+GL EGT +G LANPRR+ AMA + +A GA++ GGERIG+ G Sbjct: 301 AAALARHASTLKVGDGLAEGTQMGPLANPRRVAAMADFVQDALARGATVAAGGERIGAAG 360 Query: 364 NFFAPTVIANVPLDADVFNNEPFGPVAAIRGFDKLEEAIAEANRLPFGLAGYAFTRSFAN 423 NFFAPTV+ VPLDA VFN EPFGPVAAIRGF L+EAIAEANRL FGLAGYAFTRS N Sbjct: 361 NFFAPTVLTGVPLDAKVFNEEPFGPVAAIRGFTTLDEAIAEANRLSFGLAGYAFTRSLKN 420 Query: 424 VHLLTQRLEVGMLWINQPATPWPEMPFGGVKDSGYGSEGGPEALEPYLVTKSVTVMAV 481 H L LEVGML++NQPATP EMPFGG+KDSGYG+EGGPEAL+ YL T++VTVM V Sbjct: 421 AHRLAHELEVGMLYVNQPATPSAEMPFGGIKDSGYGTEGGPEALDAYLNTRAVTVMNV 478 Lambda K H 0.318 0.134 0.393 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 828 Number of extensions: 32 Number of successful extensions: 2 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 481 Length of database: 478 Length adjustment: 34 Effective length of query: 447 Effective length of database: 444 Effective search space: 198468 Effective search space used: 198468 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 51 (24.3 bits)
This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory