Align 3-ketoacyl-CoA thiolase (EC 2.3.1.16) (characterized)
to candidate WP_004312070.1 C665_RS12455 acetyl-CoA C-acyltransferase
Query= reanno::pseudo13_GW456_L13:PfGW456L13_2982 (397 letters) >NCBI__GCF_000310185.1:WP_004312070.1 Length = 402 Score = 549 bits (1414), Expect = e-161 Identities = 276/393 (70%), Positives = 329/393 (83%), Gaps = 3/393 (0%) Query: 5 NDPIVIVSAVRTPMGGFQGELKSLTAPQLGAAAIKAAVERAGVASDSVDEVLFGCVLPAG 64 +DP+VIVS RTP+GGFQG+L SLT PQLG+ AIKAAVERAGVA V EV+ GCVLPAG Sbjct: 2 SDPVVIVSVARTPLGGFQGDLSSLTTPQLGSIAIKAAVERAGVAPAQVQEVIMGCVLPAG 61 Query: 65 LGQAPARQAALGAGLDKSTRCTTLNKMCGSGMEAAILAHDMLLAGSADVVVAGGMESMSN 124 LGQAPARQAALGAGL S CTT+NK+CGSGM+A +LAHD++LAGS +V+VAGGMESMSN Sbjct: 62 LGQAPARQAALGAGLPLSAGCTTINKVCGSGMKATMLAHDLILAGSNEVMVAGGMESMSN 121 Query: 125 APYLLDRARAGYRMGHGRVQDSMFLDGLEDAY---DKGRLMGTFAEDCAETNGFSREAQD 181 APYLL +ARAGYR+GHG++ D MFLDGLED+Y +KGRLMGTFAEDCA F+R+AQD Sbjct: 122 APYLLPKARAGYRLGHGQMFDHMFLDGLEDSYSKENKGRLMGTFAEDCAGHFNFTRQAQD 181 Query: 182 AFAIASTTRAQQAIKDGSFKAEIVPLTVTVGKEQVVISNDEQPPKARLDKIASLKPAFRE 241 FAIASTTRAQ AI +G F E+VP+TV K VV+ DEQP KA++DKI SLKPAF++ Sbjct: 182 EFAIASTTRAQAAINNGDFSWEVVPVTVASRKGDVVVDKDEQPLKAQIDKIPSLKPAFKK 241 Query: 242 GGTVTAANSSSISDGAAALVLMRQSQAQKQGLKPLAVIHGHAAFADTPGLFPVAPIGAIK 301 GTVT ANSSSISDGAAALVLMR+S A+K G+KP+A I GHA A P LF AP+GA++ Sbjct: 242 DGTVTPANSSSISDGAAALVLMRKSTAEKLGVKPVATIIGHATHAQEPALFTTAPVGAMQ 301 Query: 302 KLMKKTGWSLNDVDLVEVNEAFAVVGMAAMTHLEIPHEKLNVHGGACALGHPIGASGARI 361 K++ K GWS VDL E+NEAFAVV MAAM +++PH+K+NV+GGACALGHPIGASGARI Sbjct: 302 KVLAKAGWSTEQVDLWEINEAFAVVTMAAMHEMKLPHDKVNVNGGACALGHPIGASGARI 361 Query: 362 LVTLLSALRQKGLKRGVAAICIGGGEATAMAVE 394 LV+L+ ALR++GLKRGVA++CIGGGEATAMA+E Sbjct: 362 LVSLIGALRKRGLKRGVASLCIGGGEATAMAIE 394 Lambda K H 0.318 0.132 0.376 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 580 Number of extensions: 17 Number of successful extensions: 2 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 397 Length of database: 402 Length adjustment: 31 Effective length of query: 366 Effective length of database: 371 Effective search space: 135786 Effective search space used: 135786 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 50 (23.9 bits)
Align candidate WP_004312070.1 C665_RS12455 (acetyl-CoA C-acyltransferase)
to HMM TIGR01930 (acetyl-CoA C-acyltransferase (EC 2.3.1.16))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.carbon/TIGR01930.hmm # target sequence database: /tmp/gapView.5008.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR01930 [M=385] Accession: TIGR01930 Description: AcCoA-C-Actrans: acetyl-CoA C-acyltransferase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 2.1e-130 421.1 5.8 2.3e-130 420.9 5.8 1.0 1 lcl|NCBI__GCF_000310185.1:WP_004312070.1 C665_RS12455 acetyl-CoA C-acyltr Domain annotation for each sequence (and alignments): >> lcl|NCBI__GCF_000310185.1:WP_004312070.1 C665_RS12455 acetyl-CoA C-acyltransferase # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 420.9 5.8 2.3e-130 2.3e-130 1 385 [] 7 394 .. 7 394 .. 0.98 Alignments for each domain: == domain 1 score: 420.9 bits; conditional E-value: 2.3e-130 TIGR01930 1 ivdavRtpigklggslkelsaedLlaavikelleragldpekidevilGnvlqageqaniaReaalaag 69 iv+ +Rtp g ++g l++l+ +L++ +ik+++erag+ p+++ evi+G+vl+ag ++++aR+aal ag lcl|NCBI__GCF_000310185.1:WP_004312070.1 7 IVSVARTPLGGFQGDLSSLTTPQLGSIAIKAAVERAGVAPAQVQEVIMGCVLPAGLGQAPARQAALGAG 75 89********99********************************************************* PP TIGR01930 70 lpesvpaltvnrvCaSglqAvalaaqkikaGeadvvvaGGvEsmSrvpillkaslrreslklgkakled 138 lp s +t+n+vC+Sg++A +la + i aG ++v+vaGG+EsmS++p+ll+++ r++++lg+ ++ d lcl|NCBI__GCF_000310185.1:WP_004312070.1 76 LPLSAGCTTINKVCGSGMKATMLAHDLILAGSNEVMVAGGMESMSNAPYLLPKA--RAGYRLGHGQMFD 142 ****************************************************98..89*********** PP TIGR01930 139 qllkdl.......vktklsmgetAenlakkygisReeqDeyalrShqkaakAieegkfkdeivpvevkg 200 ++ d+ ++++ mg+ Ae +a +++++R+ qDe+a++S ++a++Ai++g f+ e+vpv+v lcl|NCBI__GCF_000310185.1:WP_004312070.1 143 HMFLDGledsyskENKGRLMGTFAEDCAGHFNFTRQAQDEFAIASTTRAQAAINNGDFSWEVVPVTVAS 211 9999999*****999999*************************************************99 PP TIGR01930 201 k..kkvvskDegirpnttlekLakLkpafkekkgstvtAgNssqlnDGAaalllmseevakelgltpla 267 + ++vv+kDe++ + +++k+ +Lkpafk+ +g tvt +Nss+++DGAaal+lm +++a++lg++p+a lcl|NCBI__GCF_000310185.1:WP_004312070.1 212 RkgDVVVDKDEQPL-KAQIDKIPSLKPAFKK-DG-TVTPANSSSISDGAAALVLMRKSTAEKLGVKPVA 277 99888899999887.899***********95.9*.6********************************* PP TIGR01930 268 rivsaavagvdpeemglgpvpAiekaLkkaglsisdidlvEinEAFAaqvlavekelgsldlekvNvnG 336 +i+++a+ + +p+ ++++pv A++k+L+kag+s++++dl+EinEAFA++++a+++e++ l ++kvNvnG lcl|NCBI__GCF_000310185.1:WP_004312070.1 278 TIIGHATHAQEPALFTTAPVGAMQKVLAKAGWSTEQVDLWEINEAFAVVTMAAMHEMK-LPHDKVNVNG 345 **********************************************************.88******** PP TIGR01930 337 GAiAlGHPlGasGarivltllkeLkergkkyGlatlCvggGqGaAvile 385 GA AlGHP+GasGari+++l+ +L++rg k+G+a+lC+ggG ++A+ +e lcl|NCBI__GCF_000310185.1:WP_004312070.1 346 GACALGHPIGASGARILVSLIGALRKRGLKRGVASLCIGGGEATAMAIE 394 **********************************************987 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (385 nodes) Target sequences: 1 (402 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.01u 0.01s 00:00:00.02 Elapsed: 00:00:00.01 # Mc/sec: 9.35 // [ok]
This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory