Alignments for a candidate for lpd in Thauera aminoaromatica S2

GapMind for catabolism of small carbon sources

Alignments for a candidate for lpd in Thauera aminoaromatica S2

Align Dihydrolipoyl dehydrogenase; Dihydrolipoamide dehydrogenase; EC 1.8.1.4 (characterized)
to candidate WP_004326337.1 C665_RS18455 hypothetical protein

Query= SwissProt::P85207
         (461 letters)



>NCBI__GCF_000310185.1:WP_004326337.1
          Length = 722

 Score =  246 bits (627), Expect = 2e-69
 Identities = 167/461 (36%), Positives = 244/461 (52%), Gaps = 29/461 (6%)

Query: 5   DLIVIGTGPGGYPAAIRGAQLGLKVLAVEAAEVGGVCLNVGCIPTKALLHAAETVHHLKG 64
           +L+VIG G  G   +   A +  KV  VEA  +GG CLN GC+P+KAL+ +A   H ++ 
Sbjct: 237 NLVVIGAGAAGLVTSYIAAAVKAKVTLVEAGRMGGDCLNTGCVPSKALIRSARLAHQIRH 296

Query: 65  AEGFGLK-AKPELDLKKLGAWRDGVVKKLTG-GVAGLLKGNKVELLRGFARFKGPREIEV 122
           A+ +GL+  +PE+D ++L A    V++K+             VE+L+G AR   P  +EV
Sbjct: 297 ADRYGLEPGEPEIDFRRLMARVRAVIRKIEPHDSVERYSALGVEVLQGRARIVDPWTVEV 356

Query: 123 ---NGET--YGAQSFIIATGSEP-------MPLKGFPFGEDVWDSTRALRVEEGIPKRLL 170
               G T     +S +IATG+ P       +   G+   + VWD+  AL   +  P+RLL
Sbjct: 357 ALNEGGTRRLSTRSIVIATGARPTVPDLPGLEAVGYLTSDTVWDAFAAL---DAPPRRLL 413

Query: 171 VIGGGAVGLELGQIYHRLGSEVTLIEYMPEILPAGDRETAALLRKALEKEGLKVRTGTKA 230
           V+GGG +G EL Q   RLGS+V L+   P++LP  D +  A  R ALE +G++V TG  A
Sbjct: 414 VLGGGPIGCELAQACARLGSQVVLVGRAPQLLPREDADVGAFARAALEADGVEVLTGVAA 473

Query: 231 VGYEKKQ-----DGLHVLLEAAQGGSQEEIVVDKILVAVGRRPRTEGLGLEKAGVKVDER 285
           +  E++      D   V+ + A GG +  I  D +L AVGR  R EG GLE  G+     
Sbjct: 474 LRCEQETAAGGTDKFLVVADPASGG-ERRIAFDTLLCAVGRSARLEGFGLEALGIPTGAT 532

Query: 286 GFIQVNARMETSAPGVYAIGDVARPPLLAHKAMKEGLVAAENAAG---KNALFDFQ-VPS 341
             +  +  ++T  P + A GDVA P    H A  +   AA NA     +    D++ VP 
Sbjct: 533 --VATDDFLQTRYPNILAAGDVAGPLQFTHVAAHQAWHAAVNALFGHLRRFRVDYRCVPH 590

Query: 342 VVYTGPEWAGVGLTEEEARKAGYNVKVGKFPFSASGRALTLGGAEGLIKVVGDAETDLLL 401
             +T PE A VGL E EAR  G   +  ++  +   RA+T G   G +KV+     D +L
Sbjct: 591 TTFTDPEVARVGLNETEARARGIAFEFTRYDLAELDRAITDGADRGFVKVLTVPGKDRIL 650

Query: 402 GVFVVGPQAGELIAEATLALEMGATVSDLGLTIHPHPTLSE 442
           G  +VG  AGEL+AE  LA++ G  ++ L  TIH +PT +E
Sbjct: 651 GATIVGEHAGELLAEFVLAMKHGLGLNKLLGTIHAYPTFAE 691



 Score = 27.3 bits (59), Expect = 0.002
 Identities = 19/53 (35%), Positives = 28/53 (52%), Gaps = 3/53 (5%)

Query: 167 KRLLVIGGGAVGLELGQIYHRLGSEVTLIE---YMPEILPAGDRETAALLRKA 216
           + L+VIG GA GL    I   + ++VTL+E      + L  G   + AL+R A
Sbjct: 236 RNLVVIGAGAAGLVTSYIAAAVKAKVTLVEAGRMGGDCLNTGCVPSKALIRSA 288


Lambda     K      H
   0.316    0.138    0.395 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 868
Number of extensions: 49
Number of successful extensions: 6
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 2
Number of HSP's successfully gapped: 2
Length of query: 461
Length of database: 722
Length adjustment: 36
Effective length of query: 425
Effective length of database: 686
Effective search space:   291550
Effective search space used:   291550
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 53 (25.0 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources