Alignments for a candidate for bkdC in Saccharomonospora cyanea NA-134

GapMind for catabolism of small carbon sources

Alignments for a candidate for bkdC in Saccharomonospora cyanea NA-134

Align Lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex; EC 2.3.1.168; Branched-chain alpha-keto acid dehydrogenase complex component E2; BCKAD-E2; BCKADE2; Dihydrolipoamide acetyltransferase component of branched-chain alpha-keto acid dehydrogenase complex; Dihydrolipoamide branched chain transacylase; Dihydrolipoyllysine-residue (2-methylpropanoyl)transferase (uncharacterized)
to candidate WP_005454511.1 SACCYDRAFT_RS05985 2-oxoglutarate dehydrogenase, E2 component, dihydrolipoamide succinyltransferase

Query= curated2:P09062
         (423 letters)



>NCBI__GCF_000244975.1:WP_005454511.1
          Length = 607

 Score =  231 bits (589), Expect = 5e-65
 Identities = 156/465 (33%), Positives = 238/465 (51%), Gaps = 57/465 (12%)

Query: 6   IKMPDIGEGIAQVELVEWFVKVGDIIAEDQVVADVMTDKATVEIPSPVSGKVLALGGQPG 65
           + +P++GE + +  +  W  +VGD +  D+ + ++ TDK   E+PSPV+G VL +     
Sbjct: 137 VTLPELGESVTEGTVTRWLKQVGDRVEVDEPLLEISTDKVDTEVPSPVAGTVLEITVGED 196

Query: 66  EVMAVGSELIRIEVEGSGNHVDV--PQAKPAEVPAAPVAAKPEPQKDVKPAAYQASAS-- 121
           E + VG++L    V GS +      P A P   P     A PEPQ + +PA  Q SA   
Sbjct: 197 ETVEVGAQLA---VVGSADAAPAQQPPAAPEPQPQQQAPAAPEPQPEPEPAPQQPSAPAP 253

Query: 122 --------------HEAAPIVP-------RQPGDKPLASPAVRKRALDAGIELRYVHGSG 160
                          EA P  P       R     P  +P VRK A + GI+L  + GSG
Sbjct: 254 QPQAQQPQAPAPAPQEAEPAKPAAAAPAERDGAGTPYVTPLVRKLAAEHGIDLSTLKGSG 313

Query: 161 PAGRILHED-LDAFMSKPQSAAGQT---------------PNGYARRTDSEQVPVIG--- 201
             GRI  +D L A  +K +  A  T               P+  A  +D+E+  + G   
Sbjct: 314 VGGRIRKQDVLAAAEAKQKEVAAPTAQQPAPAAPSAQPARPSAPAPVSDAEKAALRGTVQ 373

Query: 202 ----LRRKIAQRMQDAKRRVAHFSYVEEIDVTALEALRQQLNSKHGDSRG-KLTLLPFLV 256
               +R+  A + +++ +  A  + V E+DVT +  LRQ+      +  G  LT LPF  
Sbjct: 374 KANRIRQITAVKTKESLQVSAQLTQVHEVDVTKVAQLRQRAKGAFREREGVNLTFLPFFA 433

Query: 257 RALVVALRDFPQINATYDDEAQIITRHGAVHVGIATQGDNGLMVPVLRHAEAGSLWANAG 316
           +A V AL+  P +NA+Y++E + IT HGA+++G+A   D GL+  V+  A   SL   A 
Sbjct: 434 KATVEALKQHPNVNASYNEETKEITYHGAINLGMAVDTDRGLLSVVIHDAGELSLAGLAH 493

Query: 317 EISRLANAARNNKASREELSGSTITLTSLGALGGIVSTPVVNTPEVAIVGVNRMVERPVV 376
            I+ LA  AR+NK + +EL+G T T+T+LG+ G +  TP++  P+  I+GV  +V RPVV
Sbjct: 494 RIADLAARARSNKVTPDELTGGTFTITNLGSNGALFDTPIIVQPQSGILGVGAVVRRPVV 553

Query: 377 ---IDGQ--IVVRKMMNLSSSFDHRVVDGMDAALFIQAVRGLLEQ 416
               DG   I VR M+ L  ++DHR++DG DA  F+  ++  LE+
Sbjct: 554 ATDADGNDTIAVRSMVYLPLTYDHRLIDGADAGRFLTTIKQRLEE 598



 Score = 72.4 bits (176), Expect = 4e-17
 Identities = 42/136 (30%), Positives = 69/136 (50%), Gaps = 11/136 (8%)

Query: 6   IKMPDIGEGIAQVELVEWFVKVGDIIAEDQVVADVMTDKATVEIPSPVSGKVLALGGQPG 65
           + +P++GE + +  +  W  + GD +  D+ + ++ TDK   E+PSPV+G +  +  +  
Sbjct: 5   VTLPELGESVTEGTVTRWLKQEGDRVEVDEPLLEISTDKVDTEVPSPVAGTLSRIVARED 64

Query: 66  EVMAVGSELIRIEVEGSGNHVDVPQAKPAEVPAAPVAAKPEPQKDVKPAAYQASASHEAA 125
           E + VG EL  I+ +GSG          AE  AAP A   +P++   P A QA A  E  
Sbjct: 65  ETVEVGGELAVID-DGSGGGA-------AETAAAPEA---QPEQQAAPQAQQAEAQPEPQ 113

Query: 126 PIVPRQPGDKPLASPA 141
               +Q    P A+P+
Sbjct: 114 AAPAQQQAPAPAAAPS 129


Lambda     K      H
   0.316    0.133    0.376 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 662
Number of extensions: 33
Number of successful extensions: 6
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 2
Number of HSP's successfully gapped: 2
Length of query: 423
Length of database: 607
Length adjustment: 34
Effective length of query: 389
Effective length of database: 573
Effective search space:   222897
Effective search space used:   222897
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources