Align Exo-alpha-(1->6)-L-arabinopyranosidase; APY; Beta-D-galactopyranosidase; EC 3.2.1.- (characterized)
to candidate WP_005457463.1 SACCYDRAFT_RS15370 beta-glucosidase
Query= SwissProt::E7CY69 (757 letters) >NCBI__GCF_000244975.1:WP_005457463.1 Length = 772 Score = 232 bits (591), Expect = 6e-65 Identities = 193/654 (29%), Positives = 302/654 (46%), Gaps = 71/654 (10%) Query: 68 ATCFPPAAGLSSSWNPELIHKVGEAMAEECIQEKVAVILGPGVNIKRNPLGGRCFEYWSE 127 AT +P +++++PEL+ ++ A+ + V L P +++ R+ GR E E Sbjct: 122 ATVYPAPLAWAATFDPELVERMAAAIGADMRAVGVHQGLSPVLDVARDHRWGRVEETLGE 181 Query: 128 DPYLAGHEAIGIVEGVQSKGVGTSLKHFAANN-QETDRLRVDARISPRALREIYFPAFEH 186 DPYL G V G+QS+GV +LKHFA + R I PR LR++ P FE Sbjct: 182 DPYLVGMLGSAYVRGLQSEGVIATLKHFAGYSASRAARNHAPVPIGPRELRDVILPPFEM 241 Query: 187 IVKKAQPWTIMCSYNRINGVHSAQNHWLLTDVLRDEWGFDGIVMSDW------------- 233 V+ A ++M +Y ++GV +A + LLT VLRDEWGF+G V+SD+ Sbjct: 242 AVRDAGAGSVMNAYVDLDGVPAAADATLLTGVLRDEWGFEGTVVSDYWAVAFLATMHRVA 301 Query: 234 -GADHDRGASLNAGLNLEMPPSYT-DDQIVYAVRDGLITPAQLDRMAQGMI--------- 282 GA +L AG+++E+P + ++V VR G + A +DR A ++ Sbjct: 302 DGAADAGALALAAGIDVELPDALCYGAELVERVRRGEVDEALVDRAAMRVLRQKAELGLL 361 Query: 283 --DLVNKTRAAMSIDNYRFDVDAHDEVAHQAAIESIVMLKNDDAILPLNA--------GP 332 D ++ S+D D AH E+A + A S+V+L N D +LPL A GP Sbjct: 362 DPDWTPESDVDSSVD---LDAGAHRELARRIAERSVVLLAN-DGVLPLAAEHRRIALVGP 417 Query: 333 VANPSATPQKIAVIGEFAR----TPRYQGGGSSHITPTKMTSFLDTLAERGIKADFAPGF 388 A+ +A +G ++ PRY G G P+ + + L + I A PG Sbjct: 418 CAD-----DPLAFLGCYSYPNHVLPRYPGLGLGIEVPSLVEALRAELPDAEITA--VPG- 469 Query: 389 TLDLEPADPALESEAVETAKNADVVLMFLG-----LPEAVESEGFDRDTLDMPAKQIALL 443 + D + +EAV A ADV + +G E EG D L +P Q LL Sbjct: 470 -CGVNDDDRSRFAEAVTAADGADVCVAVVGDRAGLFGEGTSGEGCDAPDLRLPGVQEELL 528 Query: 444 EQVAAANQNVVVVLSNGSVITVAPWAKNAKGILESWLLGQSGGPALADVIFGQVSPSGKL 503 + A VVVV+ +G + A +A +++++L G+ GG ALA ++ G+ +PSG+L Sbjct: 529 SALLATGTPVVVVVVSGRPYALGAHADHAAAVVQAFLPGEEGGAALASILSGRTAPSGRL 588 Query: 504 AQSIPLDINDDPSMLNWPGEEGHVDYGEGVFAGYRYYDTYGKAVDYPFGYGLSYATFEIT 563 +P P+ P G+ +G+ +PFG+GLSY TF+ Sbjct: 589 PVQVPRHPGGQPATYLHPPLGGN---SQGI-------SNLDPTPAFPFGHGLSYTTFDYG 638 Query: 564 GVAVAKTGA---NTATVTATVTNTSDVDAAETVQVYVVPGKADVARPKHELKGFTKAFLK 620 G+ V+ T + V N+ + +E VQ+Y+ +A V RP +L GF + L Sbjct: 639 GLTVSAEQVPTDGTLDIAVDVRNSGERAGSEVVQLYLDDVQAQVTRPVRQLAGFARVDLA 698 Query: 621 AGESKTVAIDLDERAFAYWSEKYNDWHVEAGEYAIEVGVSSRDIADTVAVALDG 674 GE+ V L ++ VE G + VG S D+ + + L G Sbjct: 699 PGETARVTFTLHADRTSFTGRDLGR-IVEPGRIDVHVGRSVADLPCSASFELTG 751 Lambda K H 0.315 0.132 0.393 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 1380 Number of extensions: 78 Number of successful extensions: 8 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 757 Length of database: 772 Length adjustment: 40 Effective length of query: 717 Effective length of database: 732 Effective search space: 524844 Effective search space used: 524844 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 55 (25.8 bits)
This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory