Alignments for a candidate for lacZ in Saccharomonospora cyanea NA-134

GapMind for catabolism of small carbon sources

Alignments for a candidate for lacZ in Saccharomonospora cyanea NA-134

Align β-galactosidase (β-gal;BgaA) (EC 3.2.1.23) (characterized)
to candidate WP_005458420.1 SACCYDRAFT_RS18170 glycoside hydrolase family 2

Query= CAZy::ABW87309.1
         (574 letters)



>NCBI__GCF_000244975.1:WP_005458420.1
          Length = 586

 Score =  269 bits (687), Expect = 3e-76
 Identities = 216/583 (37%), Positives = 268/583 (45%), Gaps = 83/583 (14%)

Query: 5   PNHPRPTLQRPGWRSLEGHWDFALSEAEAPGGVRFDRKILVPFPPEAPGSGVGEAWVGVA 64
           P HPRP L RP WR+L G WD+   +    G V    +ILVP+PPE+  S +G     + 
Sbjct: 28  PEHPRPQLTRPNWRTLNGLWDYVGQDEN--GAVHRGERILVPYPPESRLSRIGRRDERM- 84

Query: 65  WYRKVLRAKP--RPGRRLFLRFGAVDYRAEVFVNGVRVLEHEGGHTPFGLDLTPFL--ER 120
           WY +     P  R GR L L FGAVD  A V+VN   +  HEGG+T F +D+T  L  + 
Sbjct: 85  WYHRTFDVPPEWRVGRVL-LHFGAVDQTATVWVNHQPLARHEGGYTAFTVDITDVLRPDA 143

Query: 121 PVEVLVRAEDDPLDPEAPRGKQALGEPGGIFYPRTTGIWQPVWLEWVPESHIAALRLTPD 180
             EVLVRA+D+        GKQA   PGGI Y   +GIWQ VWLE VP  H+  L + PD
Sbjct: 144 EQEVLVRADDEGNRGTFAVGKQA-NRPGGILYTGCSGIWQTVWLEAVPAVHVTELDMEPD 202

Query: 181 LKALGFHLEVQAQGEGEAVEVALFPGVRGEAPLGKAPWLEARFPLLGGRASGFLGLPARG 240
           L   GF L     G    V V++     G   +  A           G     L L    
Sbjct: 203 LH--GFDLVAHVAGAHGKVAVSVEVSAAGGEQVASAR----------GNPGERLRLDV-- 248

Query: 241 NPEGLLWRPETPVLFPLRLRLLSGR-RVLDEVYSYGGLREVAAQ------RGVFLLNGEP 293
            P+ LLW P+ P L+ + +R++     VLDEV SY  LR V         R V LLN  P
Sbjct: 249 -PDPLLWSPDHPHLYDVTVRVVGDDGAVLDEVASYTALRTVRVDTEGDGPRRV-LLNDRP 306

Query: 294 YFPRLVLDQGLWPEGHLAPPGLAALRRDAALAKALGFNGVRKHQKVEDPRYLHLADRLGL 353
            F    LDQG WP+G    P   ALR D    KALGFN VRKH KVE  R+ H AD+LGL
Sbjct: 307 VFLNAPLDQGYWPDGIYTAPTDEALRFDLERIKALGFNSVRKHVKVEPMRWYHWADKLGL 366

Query: 354 LVFAEMPSF--FRFSPK---------AARRYLAELAEALERDRNHPSLVAWILFNEGWGL 402
           LV+ +MPS      +P          A  R+  EL   L + RN PS+V W++FNEGWG 
Sbjct: 367 LVWQDMPSLPVVLDNPPGPQQPPVVVARERFETELRAMLHQLRNVPSIVVWVVFNEGWG- 425

Query: 403 TPWRAETLAFLQGAFLLARALDPTRPLVDNDGFE------HGPFWDLLTVHDYAPPEVLA 456
                +T    +      +A DP R +V   G             D+   H Y       
Sbjct: 426 ---EYDTARITEE----VKATDPGRLVVAASGVNCCHSHPDSGAGDVYDDHTYV------ 472

Query: 457 RRYGEGLPEAPMGRPLALAGLPPGVRPFLSEFGGLRL-----KGPGPGWGYREAEDEEAF 511
              G G PE     P A+            EFGG+ L       PG    Y    D +  
Sbjct: 473 ---GPGRPELAATDPRAIVD---------GEFGGVGLVVEGHLWPGEPMAYEMVSDRDRL 520

Query: 512 LR---EVLRYLETACGSRLSGFCYTQLYDTFQEENGLLDFWRR 551
                E+   LE     RLSG  YTQ+ D   E NGLL + RR
Sbjct: 521 TERYVELSTELEALARDRLSGAVYTQITDVENEVNGLLTYDRR 563


Lambda     K      H
   0.322    0.144    0.467 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1080
Number of extensions: 68
Number of successful extensions: 8
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 574
Length of database: 586
Length adjustment: 36
Effective length of query: 538
Effective length of database: 550
Effective search space:   295900
Effective search space used:   295900
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 53 (25.0 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources