Align Purine nucleoside phosphorylase 1; PNP 1; Inosine phosphorylase; Inosine-guanosine phosphorylase; Purine nucleoside phosphorylase I; PNP I; Pu-NPase I; EC 2.4.2.1 (characterized)
to candidate WP_005811031.1 DHAF_RS17210 purine-nucleoside phosphorylase
Query= SwissProt::P77834 (274 letters) >NCBI__GCF_000021925.1:WP_005811031.1 Length = 277 Score = 337 bits (863), Expect = 2e-97 Identities = 162/267 (60%), Positives = 204/267 (76%) Query: 6 IEQAAQFLKEKFPTSPQIGLILGSGLGVLADEIEQAIKIPYSDIPNFPVSTVEGHAGQLV 65 + + +F+ EK PQ+G+ILGSGLG D IE + IPY +IPNFPVSTVEGH GQLV Sbjct: 11 LSETRRFIIEKVNIEPQMGVILGSGLGGFVDLIEDKVVIPYQEIPNFPVSTVEGHKGQLV 70 Query: 66 YGQLEGATVVVMQGRFHYYEGYSFDKVTFPVRVMKALGVEQLIVTNAAGGVNESFEPGDL 125 +G++ G TVV MQGRFH+YEGYS VTFPVRVM+ LGV LIVTNAAGG+N ++ PGDL Sbjct: 71 FGKVLGKTVVAMQGRFHFYEGYSMQSVTFPVRVMQVLGVSGLIVTNAAGGINPAYRPGDL 130 Query: 126 MIISDHINNMGGNPLIGPNDSALGVRFPDMSEAYSKRLRQLAKDVANDIGLRVREGVYVA 185 ++I DHIN MG NPL G N S LG RFPD+SE Y RQ A +A ++G+ +EGVY A Sbjct: 131 ILIKDHINMMGDNPLRGANLSNLGPRFPDLSEGYDLEWRQKALTIAREVGIHPQEGVYAA 190 Query: 186 NTGPAYETPAEIRMIRVMGGDAVGMSTVPEVIVARHAGMEVLGISCISNMAAGILDQPLT 245 +GP+YE+PAEIR +R +G D VGMSTVPEVI+A H GM VLGISC++NMAAGIL Q L+ Sbjct: 191 MSGPSYESPAEIRFLRTIGADLVGMSTVPEVIIANHGGMRVLGISCVTNMAAGILAQRLS 250 Query: 246 HDEVIETTEKVKADFLRFVKAIVRNMA 272 H EV+ET E+++ F+RFV+A+V+ +A Sbjct: 251 HAEVMETAERIEKQFVRFVQALVKGLA 277 Lambda K H 0.319 0.137 0.388 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 283 Number of extensions: 8 Number of successful extensions: 1 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 274 Length of database: 277 Length adjustment: 25 Effective length of query: 249 Effective length of database: 252 Effective search space: 62748 Effective search space used: 62748 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 47 (22.7 bits)
Align candidate WP_005811031.1 DHAF_RS17210 (purine-nucleoside phosphorylase)
to HMM TIGR01700 (purine nucleoside phosphorylase I, inosine and guanosine-specific (EC 2.4.2.1))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.carbon/TIGR01700.hmm # target sequence database: /tmp/gapView.7563.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR01700 [M=249] Accession: TIGR01700 Description: PNPH: purine nucleoside phosphorylase I, inosine and guanosine-specific Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 1.8e-117 377.2 0.8 2e-117 377.0 0.8 1.0 1 lcl|NCBI__GCF_000021925.1:WP_005811031.1 DHAF_RS17210 purine-nucleoside p Domain annotation for each sequence (and alignments): >> lcl|NCBI__GCF_000021925.1:WP_005811031.1 DHAF_RS17210 purine-nucleoside phosphorylase # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 377.0 0.8 2e-117 2e-117 1 248 [. 27 273 .. 27 274 .. 0.99 Alignments for each domain: == domain 1 score: 377.0 bits; conditional E-value: 2e-117 TIGR01700 1 diaiilGsGlGelaekvedavildyseiPefpestveGhkGklvfGklegkkvvilqGrfhlyegydla 69 ++++ilGsGlG +++ +ed+v+++y+eiP+fp stveGhkG+lvfGk+ gk+vv++qGrfh+yegy+++ lcl|NCBI__GCF_000021925.1:WP_005811031.1 27 QMGVILGSGLGGFVDLIEDKVVIPYQEIPNFPVSTVEGHKGQLVFGKVLGKTVVAMQGRFHFYEGYSMQ 95 579****************************************************************** PP TIGR01700 70 kvtfPvrvlkllGvealvvtnaaGgintefkvGdlmlikdhinllalnPliGpneekfGarfpdmsday 138 vtfPvrv+++lGv l+vtnaaGgin+ +++Gdl+likdhin+++ nPl+G+n +++G+rfpd+s+ y lcl|NCBI__GCF_000021925.1:WP_005811031.1 96 SVTFPVRVMQVLGVSGLIVTNAAGGINPAYRPGDLILIKDHINMMGDNPLRGANLSNLGPRFPDLSEGY 164 ********************************************************************* PP TIGR01700 139 dkelrqkakeiakelditlkeGvyvavtGPsyetpaevrllkklGadavGmstvpevivarhcGlrvlg 207 d e+rqka ia+e++i+ +eGvy a++GPsye+pae+r+l+++Gad+vGmstvpevi+a+h G+rvlg lcl|NCBI__GCF_000021925.1:WP_005811031.1 165 DLEWRQKALTIAREVGIHPQEGVYAAMSGPSYESPAEIRFLRTIGADLVGMSTVPEVIIANHGGMRVLG 233 ********************************************************************* PP TIGR01700 208 lslitnkaagildaelsdheevlevakkakekleklvsalv 248 +s++tn+aagil ++ls h ev+e+a+++++++ ++v+alv lcl|NCBI__GCF_000021925.1:WP_005811031.1 234 ISCVTNMAAGILAQRLS-HAEVMETAERIEKQFVRFVQALV 273 *****************.*******************9998 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (249 nodes) Target sequences: 1 (277 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.01u 0.01s 00:00:00.02 Elapsed: 00:00:00.01 # Mc/sec: 6.67 // [ok]
This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory