GapMind for Amino acid biosynthesis

 

Alignments for a candidate for dapF in Caminibacter mediatlanticus TB-2

Align Diaminopimelate epimerase; DAP epimerase; EC 5.1.1.7; PLP-independent amino acid racemase (uncharacterized)
to candidate WP_007474515.1 CMTB2_RS05090 diaminopimelate epimerase

Query= curated2:A6Q160
         (250 letters)



>NCBI__GCF_000170735.1:WP_007474515.1
          Length = 241

 Score =  297 bits (760), Expect = 2e-85
 Identities = 155/247 (62%), Positives = 185/247 (74%), Gaps = 7/247 (2%)

Query: 1   MQLVKYSASGNDFVIYHTFVKKDRSELAKLLCHRHEGIGADGLIVLLPHSRYDFEWQFYN 60
           M + KYSASGNDFVI+HTF KKDRS LAK LC R  GIGADGLIVLLPH  YDFEWQFYN
Sbjct: 1   MFVCKYSASGNDFVIFHTFKKKDRSNLAKKLCDRFNGIGADGLIVLLPHKTYDFEWQFYN 60

Query: 61  ADGSEAEMCGNGSRAAAHYAYSYGLASKQMRFLTLAGVIEASVEADVVESQLTKPKILDR 120
           ADGSEA MCGNGSRAAA YAY Y LAS +M+FLT AGVIEA V+ D+VE+QLT  K L +
Sbjct: 61  ADGSEASMCGNGSRAAALYAYKYDLASSKMKFLTGAGVIEAEVDGDIVETQLTPYKELGK 120

Query: 121 KIEENGKRWWLIDTGVPHLVTFVEDLNQFDKNESKRLRNKYNANVNYGIIRDLENVGVRT 180
             E     W L DTGVPHLV  ++ L  F+ NE + LR K+NANVN   ++D   + VRT
Sbjct: 121 FDE-----WELFDTGVPHLVK-LDKLENFNINECRELRYKHNANVNIAEVKD-NKLFVRT 173

Query: 181 YERGVEDETLACGTGMAATFLRAFEEGVVNPTVTVVPKSGEELQLRYENEKLFFKGRVKK 240
           YERGVEDETLACGTGM A+FL+A +EG++  ++ V PKS EEL++  +  +L+FKGRVK+
Sbjct: 174 YERGVEDETLACGTGMCASFLKARKEGLIGDSIKVYPKSKEELEISLKRNRLYFKGRVKE 233

Query: 241 VFETFKE 247
            F+   E
Sbjct: 234 TFKALCE 240


Lambda     K      H
   0.319    0.136    0.404 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 260
Number of extensions: 12
Number of successful extensions: 4
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 250
Length of database: 241
Length adjustment: 24
Effective length of query: 226
Effective length of database: 217
Effective search space:    49042
Effective search space used:    49042
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 46 (22.3 bits)

Align candidate WP_007474515.1 CMTB2_RS05090 (diaminopimelate epimerase)
to HMM TIGR00652 (dapF: diaminopimelate epimerase (EC 5.1.1.7))

Software error:

../bin/blast/fastacmd -i /tmp/list.2657.in -d ../tmp/orgsDef_201/orgs.faa -p T > /tmp/gapView.2657.genome.faa failed: Inappropriate ioctl for device at ../lib/pbutils.pm line 379.

For help, please send mail to the webmaster (help@microbesonline.org), giving this error message and the time and date of the error.