Alignments for a candidate for rhaD in Klebsiella variicola At-22

GapMind for catabolism of small carbon sources

Alignments for a candidate for rhaD in Klebsiella variicola At-22

Align Rhamnulose-1-phosphate aldolase (EC 4.1.2.19) (characterized)
to candidate WP_008807883.1 KVAR_RS25120 rhamnulose-1-phosphate aldolase

Query= reanno::Koxy:BWI76_RS00670
         (276 letters)



>NCBI__GCF_000025465.1:WP_008807883.1
          Length = 276

 Score =  545 bits (1404), Expect = e-160
 Identities = 267/275 (97%), Positives = 267/275 (97%)

Query: 1   MQTIIDAWFVQGMIKATSDAWLKGWDERNGGNLTLRLDEADIEPYAADFHAKPRYIALSQ 60
           MQTIIDAWFVQGMIKATSDAWLKGWDERNGGNLTLRLDEADIEPYAADFHAKPRYIALSQ
Sbjct: 1   MQTIIDAWFVQGMIKATSDAWLKGWDERNGGNLTLRLDEADIEPYAADFHAKPRYIALSQ 60

Query: 61  PMPTLANQPFIVTGSGKFFRNVQLDPAANLGVVKVDSDGAGYHILWGLTNDAVPTSELPA 120
           PMP LANQPFIVTGSGKFFRNVQLDPAANLGVVKVDSDGAGYHILWGLT DAVPTSELPA
Sbjct: 61  PMPILANQPFIVTGSGKFFRNVQLDPAANLGVVKVDSDGAGYHILWGLTEDAVPTSELPA 120

Query: 121 HFLSHSERIKLTNGKDRVIMHCHATNLIALTYVLENSSDLFTRKLWEGSTECLVVFPDGV 180
           HFLSHSERIKLT GKDRVIMHCHATNLIALTYVLEN SDLFTRKLWEGSTECLVVFPDGV
Sbjct: 121 HFLSHSERIKLTGGKDRVIMHCHATNLIALTYVLENHSDLFTRKLWEGSTECLVVFPDGV 180

Query: 181 GILPWMVPGTDEIGQATAEAMQKHSLVLWPFHGVFGSGPNLDETFGLIDTAEKSAEVLVK 240
           GILPWMVPGTDEIGQATAE MQKHSLVLWPFHGVFGSGP LDETFGLIDTAEKSAEVLVK
Sbjct: 181 GILPWMVPGTDEIGQATAETMQKHSLVLWPFHGVFGSGPTLDETFGLIDTAEKSAEVLVK 240

Query: 241 VYSMGGMKQTITREELIALGKRFNVHPLQSALDLY 275
           V SMGGMKQTITREELIALGKRFNV PLQSALDLY
Sbjct: 241 VLSMGGMKQTITREELIALGKRFNVQPLQSALDLY 275


Lambda     K      H
   0.319    0.136    0.420 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 382
Number of extensions: 6
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 276
Length of database: 276
Length adjustment: 25
Effective length of query: 251
Effective length of database: 251
Effective search space:    63001
Effective search space used:    63001
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 47 (22.7 bits)

Align candidate WP_008807883.1 KVAR_RS25120 (rhamnulose-1-phosphate aldolase)
to HMM TIGR02624 (rhaD: rhamnulose-1-phosphate aldolase (EC 4.1.2.19))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.carbon/TIGR02624.hmm
# target sequence database:        /tmp/gapView.22092.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR02624  [M=270]
Accession:   TIGR02624
Description: rhamnu_1P_ald: rhamnulose-1-phosphate aldolase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                                 Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                                 -----------
   5.1e-140  451.4   0.0   5.7e-140  451.2   0.0    1.0  1  lcl|NCBI__GCF_000025465.1:WP_008807883.1  KVAR_RS25120 rhamnulose-1-phosph


Domain annotation for each sequence (and alignments):
>> lcl|NCBI__GCF_000025465.1:WP_008807883.1  KVAR_RS25120 rhamnulose-1-phosphate aldolase
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  451.2   0.0  5.7e-140  5.7e-140       1     270 []       1     272 [.       1     272 [. 0.99

  Alignments for each domain:
  == domain 1  score: 451.2 bits;  conditional E-value: 5.7e-140
                                 TIGR02624   1 mqdildavfvkemikttedlyrkGwdernGGnislrlkeeeveayld.lkqvlrkiplkfdakelanky 68 
                                               mq i+da+fv++mik+t+d++ kGwdernGGn++lrl+e+++e+y+  ++  +r+i l+++++ lan+ 
  lcl|NCBI__GCF_000025465.1:WP_008807883.1   1 MQTIIDAWFVQGMIKATSDAWLKGWDERNGGNLTLRLDEADIEPYAAdFHAKPRYIALSQPMPILANQP 69 
                                               789******************************************876********************* PP

                                 TIGR02624  69 flvtGsGkyfknveeapaenlgllrvsedGksvellwGltdgglptselpahflshiarlkvdp.enrv 136
                                               f+vtGsGk+f+nv+ +pa+nlg+++v++dG+ +++lwGlt++++ptselpahflsh +r+k ++ ++rv
  lcl|NCBI__GCF_000025465.1:WP_008807883.1  70 FIVTGSGKFFRNVQLDPAANLGVVKVDSDGAGYHILWGLTEDAVPTSELPAHFLSHSERIKLTGgKDRV 138
                                               *************************************************************9998**** PP

                                 TIGR02624 137 imhchatnlialtftleldekaftrtlwemsteclvvfpeGvGiipwlvpGtdeiGeataekmkesrlv 205
                                               imhchatnlialt++le+++  ftr+lwe+steclvvfp+GvGi+pw+vpGtdeiG+atae m++++lv
  lcl|NCBI__GCF_000025465.1:WP_008807883.1 139 IMHCHATNLIALTYVLENHSDLFTRKLWEGSTECLVVFPDGVGILPWMVPGTDEIGQATAETMQKHSLV 207
                                               ********************************************************************* PP

                                 TIGR02624 206 lwphhGifgaGesldevfGlietaekaaevytkvlsqggvkqtisdedlialaerfdvkpkakyl 270
                                               lwp+hG+fg+G++lde+fGli+taek+aev++kvls+gg+kqti++e+lial++rf+v+p +++l
  lcl|NCBI__GCF_000025465.1:WP_008807883.1 208 LWPFHGVFGSGPTLDETFGLIDTAEKSAEVLVKVLSMGGMKQTITREELIALGKRFNVQPLQSAL 272
                                               ************************************************************98875 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (270 nodes)
Target sequences:                          1  (276 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.01u 0.00s 00:00:00.01 Elapsed: 00:00:00.00
# Mc/sec: 8.87
//
[ok]

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

Downloads

Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources