Align Probable glycine dehydrogenase (decarboxylating) subunit 1; EC 1.4.4.2; Glycine cleavage system P-protein subunit 1; Glycine decarboxylase subunit 1; Glycine dehydrogenase (aminomethyl-transferring) subunit 1 (uncharacterized)
to candidate WP_010959710.1 MCA_RS01720 glycine dehydrogenase
Query= curated2:B8GN16 (451 letters) >NCBI__GCF_000008325.1:WP_010959710.1 Length = 453 Score = 588 bits (1516), Expect = e-172 Identities = 283/451 (62%), Positives = 348/451 (77%), Gaps = 1/451 (0%) Query: 1 MPFIPHTETDIQDMLAAIGVNDIEQLFDEIPSALRTGPLNLVPEAMSEMEITRLMHRRAA 60 MPFIPHTE +++DMLAAIG I++LF EIP LR G L +PEA+SEME+ +LM RAA Sbjct: 1 MPFIPHTEAEVRDMLAAIGAGSIDELFAEIPPDLRCGELKDLPEALSEMEVCKLMEERAA 60 Query: 61 QDHVDLNFIGAGAYEHHIPAAVWQIATRGEFYSAYTPYQAEASQGTLQLVYEYQTMMTGL 120 ++ L F GAGAYEH+IPAAVW+IA RGEFYSAYTPYQAEASQG+LQ+ YEYQ+MM GL Sbjct: 61 ENRSALCFAGAGAYEHYIPAAVWEIALRGEFYSAYTPYQAEASQGSLQVFYEYQSMMAGL 120 Query: 121 TGMDVSNASLYDGASALAEAVLMSVRANRKSKSHRVLMPATVNPVYRSVTRAIVHGQGIE 180 MDVSNASLYDGASAL EA+LM++R N +S+S ++L+P ++NPVY+ V R + Q +E Sbjct: 121 MAMDVSNASLYDGASALGEAILMALRTNPESRSRKILLPRSLNPVYKRVVRTLTRNQRVE 180 Query: 181 LVEVPFDPATGRVDPKSLAAFEGQDITALVIPQPNFFGVLEEADALTDWAHAQDAQVIGV 240 LV++ +D + GR+ +L FEG + A+VIPQPN FGVLEE D L DWAH A+ I V Sbjct: 181 LVDLDYDRSLGRIAENALETFEGGEFAAVVIPQPNHFGVLEEVDVLADWAHRHGARSIAV 240 Query: 241 VNPVSLALLKPPGEWGGKGADIVCGEGQPLGAPLSSGGPYFGFMCCRKEHVRQMPGRIVG 300 VNP ++ALLKPPGEWG GAD+ CGEGQPLG PLS+GGPYFGF+C R++ V Q+PGR+VG Sbjct: 241 VNPTAMALLKPPGEWGEHGADLACGEGQPLGIPLSAGGPYFGFLCSRQDFVHQLPGRLVG 300 Query: 301 RTLDADGRTGFVLTLQAREQHIRRSKATSNICTNQGLVVTAATIHMALLGPEGLERVAAA 360 RT+D DGR GF LTLQ REQHIRR KATSNICTNQGL+VTAAT++MAL+GP+GL VAAA Sbjct: 301 RTVDVDGREGFTLTLQPREQHIRRGKATSNICTNQGLMVTAATLYMALMGPKGLRNVAAA 360 Query: 361 CHANTHALIERLKPL-GVTPAFAGEAFHEIAFRLPRPVRPVLDAMAQQGVLGGYALGDDY 419 CHAN AL+ERL + GV P F FHE A RLPR VL +A++G+LGG+ L DY Sbjct: 361 CHANASALLERLTRIDGVEPVFPAPFFHEAAIRLPRAADRVLAGLAERGILGGHVLSADY 420 Query: 420 SDLGDLLLVCATETKTEADLDRYAEVMKAAL 450 +LGD LL+CATET+ D+DRYA + L Sbjct: 421 PELGDALLICATETRGPEDMDRYAAALAEVL 451 Lambda K H 0.320 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 606 Number of extensions: 17 Number of successful extensions: 2 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 451 Length of database: 453 Length adjustment: 33 Effective length of query: 418 Effective length of database: 420 Effective search space: 175560 Effective search space used: 175560 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 51 (24.3 bits)
This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory