Alignments for a candidate for tpi in Methylococcus capsulatus Bath

GapMind for catabolism of small carbon sources

Alignments for a candidate for tpi in Methylococcus capsulatus Bath

Align Triosephosphate isomerase; TIM; TPI; Triose-phosphate isomerase; EC 5.3.1.1 (characterized)
to candidate WP_010960015.1 MCA_RS03330 triose-phosphate isomerase

Query= SwissProt::P50921
         (256 letters)



>NCBI__GCF_000008325.1:WP_010960015.1
          Length = 250

 Score =  233 bits (595), Expect = 2e-66
 Identities = 121/252 (48%), Positives = 167/252 (66%), Gaps = 3/252 (1%)

Query: 1   MRHPVVMGNWKLNGSKEMVVDLLNGLNAELEGVTGVDVAVAPPALFVDLAERTLTEAGSA 60
           MR P+V+GNWK+NG    V  LLN + A + G    +V V  P +++  A   L   G+ 
Sbjct: 1   MRRPLVVGNWKMNGRSASVARLLNDILAGI-GDCKAEVGVCVPFVYIPQASEILK--GTK 57

Query: 61  IILGAQNTDLNNSGAFTGDMSPAMLKEFGATHIIIGHSERREYHAESDEFVAKKFAFLKE 120
           ++LGAQN   +NSGAFTG++S  ML+EFG    I+GHSERR  + ES+E VA ++    +
Sbjct: 58  VMLGAQNVADHNSGAFTGEISAGMLREFGCELAIVGHSERRLLYGESNELVASRYEQAIQ 117

Query: 121 NGLTPVLCIGESDAQNEAGETMAVCARQLDAVINTQGVEALEGAIIAYEPIWAIGTGKAA 180
             L P+LC+GE+  Q E G T+AV   Q+D V    GV++LE A+IAYEP+WA+GTG++A
Sbjct: 118 GHLKPILCVGETLEQREQGRTLAVIGAQIDTVFEFAGVQSLEHAVIAYEPVWAVGTGRSA 177

Query: 181 TAEDAQRIHAQIRAHIAEKSEAVAKNVVIQYGGSVKPENAAAYFAQPDIDGALVGGAALD 240
           T   AQ +H  IR+ IA  +  VA+ V I YGGSVKPEN+A  FA PDIDG L+GGA+LD
Sbjct: 178 TTGQAQEVHYHIRSLIARWNPEVAQAVQIIYGGSVKPENSAELFAMPDIDGGLIGGASLD 237

Query: 241 AKSFAAIAKAAA 252
           A++F +I  + +
Sbjct: 238 ARAFLSICHSVS 249


Lambda     K      H
   0.314    0.130    0.364 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 168
Number of extensions: 5
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 256
Length of database: 250
Length adjustment: 24
Effective length of query: 232
Effective length of database: 226
Effective search space:    52432
Effective search space used:    52432
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.5 bits)
S2: 46 (22.3 bits)

Align candidate WP_010960015.1 MCA_RS03330 (triose-phosphate isomerase)
to HMM TIGR00419 (tpiA: triose-phosphate isomerase (EC 5.3.1.1))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.carbon/TIGR00419.hmm
# target sequence database:        /tmp/gapView.10917.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR00419  [M=228]
Accession:   TIGR00419
Description: tim: triose-phosphate isomerase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                                 Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                                 -----------
    2.5e-71  226.1   0.2    2.9e-71  225.9   0.2    1.0  1  lcl|NCBI__GCF_000008325.1:WP_010960015.1  MCA_RS03330 triose-phosphate iso


Domain annotation for each sequence (and alignments):
>> lcl|NCBI__GCF_000008325.1:WP_010960015.1  MCA_RS03330 triose-phosphate isomerase
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  225.9   0.2   2.9e-71   2.9e-71       1     227 [.       5     238 ..       5     239 .. 0.98

  Alignments for each domain:
  == domain 1  score: 225.9 bits;  conditional E-value: 2.9e-71
                                 TIGR00419   1 lviinfKlnesvgkvelevaklaeevaseagvevavappfvdldvvkdeve.seiqvaAqnvdavksGa 68 
                                               lv++n+K+n+   +v+ +++++ + + + ++ ev+v +pfv+++ +++ ++ ++++++Aqnv  ++sGa
  lcl|NCBI__GCF_000008325.1:WP_010960015.1   5 LVVGNWKMNGRSASVARLLNDILAGIGD-CKAEVGVCVPFVYIPQASEILKgTKVMLGAQNVADHNSGA 72 
                                               79***********************976.789*******************99**************** PP

                                 TIGR00419  69 ftGeisAemlkdlGakgvligHsErRsllkeadeliekkvarlkelglksvvCvgetleere....... 130
                                               ftGeisA ml+++G++  ++gHsErR l  e++el++ +  ++ +  lk+++Cvgetle+re       
  lcl|NCBI__GCF_000008325.1:WP_010960015.1  73 FTGEISAGMLREFGCELAIVGHSERRLLYGESNELVASRYEQAIQGHLKPILCVGETLEQREqgrtlav 141
                                               **************************************************************9999999 PP

                                 TIGR00419 131 aartinnvattaaaaAlepdvvAvEPveliGtGkpvskAeaevveksvrdhlkkvskevaesvrvlyGa 199
                                                + +i++v + a++  le +v+A+EPv+++GtG++++  +a++v+  +r  +++ ++eva+ v+++yG+
  lcl|NCBI__GCF_000008325.1:WP_010960015.1 142 IGAQIDTVFEFAGVQSLEHAVIAYEPVWAVGTGRSATTGQAQEVHYHIRSLIARWNPEVAQAVQIIYGG 210
                                               999****************************************************************** PP

                                 TIGR00419 200 svtaaedaelaaqldvdGvLlasavlka 227
                                               sv+ +++ael+a +d+dG L+++a+l a
  lcl|NCBI__GCF_000008325.1:WP_010960015.1 211 SVKPENSAELFAMPDIDGGLIGGASLDA 238
                                               *************************987 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (228 nodes)
Target sequences:                          1  (250 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.00u 0.00s 00:00:00.00 Elapsed: 00:00:00.00
# Mc/sec: 8.77
//
[ok]

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

Downloads

Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources