Alignments for a candidate for arcB in Methylococcus capsulatus Bath

GapMind for catabolism of small carbon sources

Alignments for a candidate for arcB in Methylococcus capsulatus Bath

Align Ornithine carbamoyltransferase, anabolic; OTCase; EC 2.1.3.3 (characterized)
to candidate WP_010961323.1 MCA_RS10185 ornithine carbamoyltransferase

Query= SwissProt::P11724
         (305 letters)



>NCBI__GCF_000008325.1:WP_010961323.1
          Length = 298

 Score =  388 bits (997), Expect = e-113
 Identities = 197/299 (65%), Positives = 227/299 (75%), Gaps = 3/299 (1%)

Query: 1   MSVRHFLSFMDYSPEELIGLIRRGSELKDLRNRGVLYEPLKSRVLGMVFEKASTRTRLSF 60
           M  RHF++  D S  E   LI R     DL+ R   YEPLK++VLGMVFEK+STRTR+SF
Sbjct: 1   MKPRHFVTLRDLSSAEFRALIARAI---DLKARKEPYEPLKNKVLGMVFEKSSTRTRVSF 57

Query: 61  EAGMIQLGGQAIFLSPRDTQLGRGEPIGDSARVMSRMLDGVMIRTFAHATLTEFAAHSKV 120
           E GM Q GG +IFLSPRDTQLGRGEPI DSARV+SRM+D +M+RT AH T+  FA +S+V
Sbjct: 58  EVGMAQFGGSSIFLSPRDTQLGRGEPIEDSARVLSRMVDCIMLRTHAHRTVEIFAEYSRV 117

Query: 121 PVINGLSDDLHPCQLLADMQTFHEHRGSIQGKTVAWIGDGNNMCNSYIEAALKFDFQLRV 180
           PVINGL+D  HPCQLLADMQT+ EHRG I GKTVAWIGDGNNMC +Y+ AA   DF+LR+
Sbjct: 118 PVINGLTDRFHPCQLLADMQTYFEHRGDIAGKTVAWIGDGNNMCQTYVHAAGLLDFRLRI 177

Query: 181 ACPEGYEPKAEFVALAGDRLRVVRDPREAVAGAHLVSTDVWASMGQEDEAAARIALFRPY 240
           ACP GYEP+AE V  AG  + V  D R AV GA LV TDVWASMGQE E   R   FR Y
Sbjct: 178 ACPPGYEPEAELVEAAGKCVEVGHDVRTAVNGADLVVTDVWASMGQESEQTERTGAFRDY 237

Query: 241 QVNAALLDGAADDVLFMHCLPAHRGEEISEELLDDPRSVAWDQAENRLHAQKALLELLI 299
           QVNAAL+  A  D LFMHCLPAHRGEE+S E+L+ P+SV WD+AENRLHAQKALLE L+
Sbjct: 238 QVNAALMALAHADALFMHCLPAHRGEEVSAEVLEGPQSVVWDEAENRLHAQKALLEFLL 296


Lambda     K      H
   0.322    0.136    0.404 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 331
Number of extensions: 9
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 305
Length of database: 298
Length adjustment: 27
Effective length of query: 278
Effective length of database: 271
Effective search space:    75338
Effective search space used:    75338
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 48 (23.1 bits)

Align candidate WP_010961323.1 MCA_RS10185 (ornithine carbamoyltransferase)
to HMM TIGR00658 (argF: ornithine carbamoyltransferase (EC 2.1.3.3))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.carbon/TIGR00658.hmm
# target sequence database:        /tmp/gapView.21725.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR00658  [M=304]
Accession:   TIGR00658
Description: orni_carb_tr: ornithine carbamoyltransferase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                                 Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                                 -----------
   3.3e-121  390.1   0.0   3.7e-121  389.9   0.0    1.0  1  lcl|NCBI__GCF_000008325.1:WP_010961323.1  MCA_RS10185 ornithine carbamoylt


Domain annotation for each sequence (and alignments):
>> lcl|NCBI__GCF_000008325.1:WP_010961323.1  MCA_RS10185 ornithine carbamoyltransferase
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  389.9   0.0  3.7e-121  3.7e-121       1     303 [.       4     296 ..       4     297 .. 0.98

  Alignments for each domain:
  == domain 1  score: 389.9 bits;  conditional E-value: 3.7e-121
                                 TIGR00658   1 rhllslldlseeelkellelakklkkekkkgkeekklkgktlaliFekrstRtRvsfevaayelGaqvl 69 
                                               rh+++l dls++e++ l+ +a +lk++k+     + lk+k l+++Fek+stRtRvsfev+++++G+ ++
  lcl|NCBI__GCF_000008325.1:WP_010961323.1   4 RHFVTLRDLSSAEFRALIARAIDLKARKEP---YEPLKNKVLGMVFEKSSTRTRVSFEVGMAQFGGSSI 69 
                                               8************************99887...789********************************* PP

                                 TIGR00658  70 ylnkeelqlgrkesikDtarvlsryvdaivvRvykhedveelakyasvPvingLtdlehPcqilaDllt 138
                                               +l++ ++qlgr+e+i+D arvlsr+vd+i++R+++h++ve +a+y+ vPvingLtd +hPcq+laD++t
  lcl|NCBI__GCF_000008325.1:WP_010961323.1  70 FLSPRDTQLGRGEPIEDSARVLSRMVDCIMLRTHAHRTVEIFAEYSRVPVINGLTDRFHPCQLLADMQT 138
                                               ********************************************************************* PP

                                 TIGR00658 139 ikeklgklkevklvyvGDannvanslllaaaklGldvvvatPeglepeaeivkkakkiakenggklelt 207
                                                 e+ g++ + +++++GD+nn++++ + aa +l + +++a+P+g+epeae+v+ a       g  +e+ 
  lcl|NCBI__GCF_000008325.1:WP_010961323.1 139 YFEHRGDIAGKTVAWIGDGNNMCQTYVHAAGLLDFRLRIACPPGYEPEAELVEAA-------GKCVEVG 200
                                               ****************************************************999.......558999* PP

                                 TIGR00658 208 edpkkavkdadviytDvwvsmGeeekkeerlkllkpyqvneellelakpevkflhCLPavrGeevtdev 276
                                               +d+  av++ad+++tDvw+smG+e+++ er  ++++yqvn  l++la+ ++ f+hCLPa+rGeev+ ev
  lcl|NCBI__GCF_000008325.1:WP_010961323.1 201 HDVRTAVNGADLVVTDVWASMGQESEQTERTGAFRDYQVNAALMALAHADALFMHCLPAHRGEEVSAEV 269
                                               ********************************************************************* PP

                                 TIGR00658 277 legeasivfdeaenRlhaqkavlkall 303
                                               leg++s+v+deaenRlhaqka+l++ll
  lcl|NCBI__GCF_000008325.1:WP_010961323.1 270 LEGPQSVVWDEAENRLHAQKALLEFLL 296
                                               ************************998 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (304 nodes)
Target sequences:                          1  (298 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.00u 0.01s 00:00:00.01 Elapsed: 00:00:00.01
# Mc/sec: 7.93
//
[ok]

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources