Alignments for a candidate for aacS in Methanosarcina acetivorans C2A

GapMind for catabolism of small carbon sources

Alignments for a candidate for aacS in Methanosarcina acetivorans C2A

Align Acetoacetate--CoA ligase (EC 6.2.1.16) (characterized)
to candidate WP_011021438.1 MA_RS07380 AMP-binding protein

Query= reanno::acidovorax_3H11:Ac3H11_3009
         (578 letters)



>NCBI__GCF_000007345.1:WP_011021438.1
          Length = 550

 Score =  532 bits (1371), Expect = e-155
 Identities = 266/551 (48%), Positives = 363/551 (65%), Gaps = 7/551 (1%)

Query: 26  LIEQTIGAFFADMVARQPEREALVSVHQGRRYTYAQLQTEAHRLASALLGMGLTPGDRVG 85
           LIE ++G +F   V   P+ E ++   +  R+TY Q     + LA  LL +G+  GD VG
Sbjct: 3   LIEDSLGEYFEKQVTIDPDHEFIIYPDRNLRFTYGQFNERVNNLAKGLLAIGIKKGDHVG 62

Query: 86  IWSHNNAEWVLMQLATAQVGLVLVNINPAYRTAEVEYALNKVGCKLLVSMARFKTSDYLG 145
           IW+ N  +W+    AT+++G VLV +N AYR+ EVEY L +   K L  +  F+  DYL 
Sbjct: 63  IWAKNVPDWLTFMFATSKIGAVLVTVNTAYRSHEVEYVLKQSDMKALALIDSFREVDYLE 122

Query: 146 MLRELAPEWQGQQPGHLQAAKLPQLKTVVWIDDEAGQGADEPGLLRFTELIARGNA-ADP 204
           ++ EL PE +  + G L++ K P LK+V+++  E  +G     +   +EL+  G+   D 
Sbjct: 123 IINELVPELKSSERGRLKSKKFPYLKSVIYVGQEKHRG-----MYNTSELMLLGSHYPDD 177

Query: 205 RLAQVAAGLQATDPINIQFTSGTTGFPKGATLTHRNILNNGFFIGECMKLTPADRLCIPV 264
            L ++ A +   D IN+Q+TSGTTGFPKG  LT +NILNNG  IG+  K T  DRLC+PV
Sbjct: 178 ELKEILASVSGDDVINMQYTSGTTGFPKGVMLTSKNILNNGLSIGDRQKFTHEDRLCLPV 237

Query: 265 PLYHCFGMVLGNLACFTHGATIVYPNDGFDPLTVLQTVQDERCTGLHGVPTMFIAELDHP 324
           PL+HCFG+VLG +A  TH AT+V   + FDPL VL  VQ E+CT L+GVPTMFIAE  HP
Sbjct: 238 PLFHCFGIVLGVMAVLTHRATLVML-EVFDPLLVLAAVQKEKCTALYGVPTMFIAEYTHP 296

Query: 325 RFAEFNLSTLRTGIMAGSPCPTEVMKRVVEQMNLREITIAYGMTETSPVSCQSSTDTPLS 384
            F  F+LS+LRTGIMAGS CP E MK+VV+ M+  +IT  YG+TE SP   Q++ D P+ 
Sbjct: 297 MFDMFDLSSLRTGIMAGSTCPVEAMKKVVKDMHCHQITSVYGLTEASPGMTQTTVDDPVE 356

Query: 385 KRVSTVGQVQPHLEVKIVDPDTGAVVPIGQRGEFCTKGYSVMHGYWGDEAKTREAIDEGG 444
            RV TVG+  P +EV++VDP T  +VP    GE C +GY++M GY+    +T++ IDEGG
Sbjct: 357 LRVETVGKCFPGVEVRVVDPATNELVPPDTVGEICCRGYNIMKGYYNMPEETKKVIDEGG 416

Query: 445 WMHTGDLATMDAEGYVNIVGRIKDMVIRGGENIYPREIEEFLYRHPQVQDVQVVGVPDQK 504
           W+H+GDL T D  GY  I GRIKDM+IRGGENIYPREIEEFL+  P V+D QVVG+PD+K
Sbjct: 417 WLHSGDLGTCDELGYYRITGRIKDMIIRGGENIYPREIEEFLHAIPGVKDAQVVGIPDKK 476

Query: 505 YGEELCAWIIAKPGTQPTEDDIRAFCKGQIAHYKVPRYIRFVTSFPMTVTGKIQKFKIRD 564
           YGE + A+ I + G   TE DIR +   +IA YKVP+++  V  +P+T +GKIQK+K+R+
Sbjct: 477 YGEIVGAFTILEKGADLTEADIRDYALSKIARYKVPKHVFIVNEYPLTASGKIQKYKLRE 536

Query: 565 EMKDQLGLEEQ 575
              + L   E+
Sbjct: 537 LAVELLKKREE 547


Lambda     K      H
   0.320    0.136    0.412 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 801
Number of extensions: 32
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 578
Length of database: 550
Length adjustment: 36
Effective length of query: 542
Effective length of database: 514
Effective search space:   278588
Effective search space used:   278588
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 53 (25.0 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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Protein sequences (fasta format)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources