Align Acetyl-coenzyme A synthetase; AcCoA synthetase; Acs; Acetate--CoA ligase; Acyl-activating enzyme; EC 6.2.1.1 (characterized)
to candidate WP_011318049.1 AVA_RS06110 acetate--CoA ligase
Query= SwissProt::P31638 (660 letters) >NCBI__GCF_000204075.1:WP_011318049.1 Length = 657 Score = 746 bits (1926), Expect = 0.0 Identities = 373/659 (56%), Positives = 478/659 (72%), Gaps = 13/659 (1%) Query: 4 IESVMQEHRVFNPPEGFASQAAIPSMEAYQALCDEAERDYEGFWARHARELLHWTKPFTK 63 IES++QE R+F+P F+ A I S+E YQ L D+A+ D + FWA A LHW + + Sbjct: 6 IESILQEKRLFHPSAEFSQNAQIKSLEDYQRLYDKAKADPQKFWAELAETELHWFQKWDT 65 Query: 64 VLDQSNAPFYKWFEDGELNASYNCLDRNLQNGNADKVAIVFEADDGSVTRVTYRELHGKV 123 VLD APF KWF G++N SYNCLDR+L +K A+++E + G +TY +LH +V Sbjct: 66 VLDWQ-APFAKWFVGGKINISYNCLDRHLTTWRKNKAALIWEGEPGDSRTLTYAQLHREV 124 Query: 124 CRFANGLKALGIRKGDRVVIYMPMSVEGVVAMQACARLGATHSVVFGGFSAKSLQERLVD 183 C+FAN LK LG++KGDRV IYMPM E +AM ACAR+GA HSVVFGGFSA++L++RL+D Sbjct: 125 CQFANVLKQLGVQKGDRVGIYMPMIPEAAIAMLACARIGAPHSVVFGGFSAEALRDRLID 184 Query: 184 VGAVALITADEQMRGGKALPLKAIADDALALGGCEAVRNVIVYRRTGGKVAWT-EGRDRW 242 A ++TAD R +PLK D ALA G +V NV+V +RTG + GRD W Sbjct: 185 AQAKVVVTADGGWRKDAIVPLKEQVDKALADGAVPSVENVLVVKRTGQDIYMQLGGRDHW 244 Query: 243 MEDVSAGQPDTCEAEPVSAEHPLFVLYTSGSTGKPKGVQHSTGGYLLWALMTMKWTFDIK 302 D+ G C AEP+ +E LFVLYTSGSTGKPKGV H+TGGY L+ MT KW FD++ Sbjct: 245 WHDLQKGASADCPAEPMDSEDLLFVLYTSGSTGKPKGVVHTTGGYNLYTHMTTKWIFDLQ 304 Query: 303 PDDLFWCTADIGWVTGHTYIAYGPLAAGATQVVFEGVPTYPNAGRFWDMIARHKVSIFYT 362 D++WCTAD+GW+TGH+YI YGPL+ GAT +++EG P N G FWD+I ++ V+IFYT Sbjct: 305 DTDVYWCTADVGWITGHSYIVYGPLSNGATTLMYEGAPRASNPGCFWDVIEKYGVNIFYT 364 Query: 363 APTAIRSLIKAAEADEKIHPKQYDLSSLRLLGTVGEPINPEAWMWYYKNIGNERCPIVDT 422 APTAIR+ IK E HP +LSSLRLLGTVGEPINPEAWMWY+K IG +RCPIVDT Sbjct: 365 APTAIRAFIKMGEH----HPNARNLSSLRLLGTVGEPINPEAWMWYHKVIGGDRCPIVDT 420 Query: 423 FWQTETGGHMITPLPGATPLVPGSCTLPLPGIMAAIVDETGHDVPNGNGGILVVKRPWPA 482 +WQTETGG MITPLPGA PGS TLP PGI+A IVD G+ VP GG L V+ PWP Sbjct: 421 WWQTETGGIMITPLPGAISTKPGSATLPFPGILADIVDLDGNTVPQNEGGYLAVRYPWPG 480 Query: 483 MIRTIWGDPERFRKSYF---PEELGGKLYLAGDGSIRDKDTGYFTIMGRIDDVLNVSGHR 539 M+RT++GDPERFR++Y+ P + G Y AGDG+ +D+D GYF +MGR+DDVLNVSGHR Sbjct: 481 MMRTVYGDPERFRRTYWEHIPPKDGNYTYFAGDGARKDED-GYFWVMGRVDDVLNVSGHR 539 Query: 540 MGTMEIESALVSNPLVAEAAVVGRPDDMTGEAICAFVVLKRSRPTGEEAVKIATELRNWV 599 +GTME+ESALVS+P VAEAAVVG+PDD+ GE + AFV L+ + ++ K+ L+ V Sbjct: 540 LGTMEVESALVSHPAVAEAAVVGKPDDLKGEEVVAFVTLEGTYQASDDLSKV---LKQHV 596 Query: 600 GKEIGPIAKPKDIRFGDNLPKTRSGKIMRRLLRSLAKGEEITQDTSTLENPAILEQLKQ 658 +EIG IA+P +IRF D LPKTRSGKIMRRLLR+LA G+E++ DTSTLE+ ++L++L++ Sbjct: 597 VQEIGAIARPGEIRFTDALPKTRSGKIMRRLLRNLAAGQEVSGDTSTLEDRSVLDKLRE 655 Lambda K H 0.319 0.136 0.422 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 1408 Number of extensions: 73 Number of successful extensions: 7 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 660 Length of database: 657 Length adjustment: 38 Effective length of query: 622 Effective length of database: 619 Effective search space: 385018 Effective search space used: 385018 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 54 (25.4 bits)
Align candidate WP_011318049.1 AVA_RS06110 (acetate--CoA ligase)
to HMM TIGR02188 (acs: acetate--CoA ligase (EC 6.2.1.1))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.carbon/TIGR02188.hmm # target sequence database: /tmp/gapView.19932.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR02188 [M=629] Accession: TIGR02188 Description: Ac_CoA_lig_AcsA: acetate--CoA ligase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 0 1026.6 0.0 0 1026.4 0.0 1.0 1 lcl|NCBI__GCF_000204075.1:WP_011318049.1 AVA_RS06110 acetate--CoA ligase Domain annotation for each sequence (and alignments): >> lcl|NCBI__GCF_000204075.1:WP_011318049.1 AVA_RS06110 acetate--CoA ligase # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 1026.4 0.0 0 0 2 628 .. 29 655 .. 28 656 .. 0.98 Alignments for each domain: == domain 1 score: 1026.4 bits; conditional E-value: 0 TIGR02188 2 aeleeykelyeeaiedpekfwaklakeelewlkpfekvldeslepkvkWfedgelnvsyncvdrhvek. 69 ++le+y++ly++a++dp+kfwa+la++el+w++++++vld++ +p++kWf++g++n+sync+drh+++ lcl|NCBI__GCF_000204075.1:WP_011318049.1 29 KSLEDYQRLYDKAKADPQKFWAELAETELHWFQKWDTVLDWQ-APFAKWFVGGKINISYNCLDRHLTTw 96 6899*************************************9.78************************ PP TIGR02188 70 rkdkvaiiwegdeegedsrkltYaellrevcrlanvlkelGvkkgdrvaiYlpmipeaviamlacaRiG 138 rk+k+a+iweg+ +g dsr+ltYa+l+revc++anvlk+lGv+kgdrv iY+pmipea+iamlacaRiG lcl|NCBI__GCF_000204075.1:WP_011318049.1 97 RKNKAALIWEGE-PG-DSRTLTYAQLHREVCQFANVLKQLGVQKGDRVGIYMPMIPEAAIAMLACARIG 163 ************.77.59*************************************************** PP TIGR02188 139 avhsvvfaGfsaealaeRivdaeaklvitadeglRggkvielkkivdealekaee.svekvlvvkrtge 206 a hsvvf+Gfsaeal++R++da+ak+v+tad+g+R++ +++lk++vd+al++ sve+vlvvkrtg+ lcl|NCBI__GCF_000204075.1:WP_011318049.1 164 APHSVVFGGFSAEALRDRLIDAQAKVVVTADGGWRKDAIVPLKEQVDKALADGAVpSVENVLVVKRTGQ 232 ***************************************************99989************* PP TIGR02188 207 evaewkegrDvwweelvekeasaecepekldsedplfiLYtsGstGkPkGvlhttgGylllaaltvkyv 275 ++ grD+ww++l + +asa+c++e++dsed+lf+LYtsGstGkPkGv+httgGy l++++t+k++ lcl|NCBI__GCF_000204075.1:WP_011318049.1 233 DIYMQLGGRDHWWHDLQK-GASADCPAEPMDSEDLLFVLYTSGSTGKPKGVVHTTGGYNLYTHMTTKWI 300 *998889**********9.6************************************************* PP TIGR02188 276 fdikdedifwCtaDvGWvtGhsYivygPLanGattllfegvptypdasrfweviekykvtifYtaPtai 344 fd++d+d++wCtaDvGW+tGhsYivygPL+nGattl++eg+p+ +++++fw+vieky+v+ifYtaPtai lcl|NCBI__GCF_000204075.1:WP_011318049.1 301 FDLQDTDVYWCTADVGWITGHSYIVYGPLSNGATTLMYEGAPRASNPGCFWDVIEKYGVNIFYTAPTAI 369 ********************************************************************* PP TIGR02188 345 RalmklgeelvkkhdlsslrvlgsvGepinpeaweWyyevvGkekcpivdtwWqtetGgilitplpgva 413 Ra++k+ge+++++++lsslr+lg+vGepinpeaw+Wy++v+G ++cpivdtwWqtetGgi+itplpg a lcl|NCBI__GCF_000204075.1:WP_011318049.1 370 RAFIKMGEHHPNARNLSSLRLLGTVGEPINPEAWMWYHKVIGGDRCPIVDTWWQTETGGIMITPLPG-A 437 *******************************************************************.6 PP TIGR02188 414 telkpgsatlPlfGieaevvdeegkeveeeeeggvLvikkpwPsmlrtiygdeerfvetYfkklkg... 479 +++kpgsatlP++Gi a++vd +g++v ++e+ g+L+++ pwP+m+rt+ygd+erf +tY+++++ lcl|NCBI__GCF_000204075.1:WP_011318049.1 438 ISTKPGSATLPFPGILADIVDLDGNTVPQNEG-GYLAVRYPWPGMMRTVYGDPERFRRTYWEHIPPkdg 505 *****************************999.8*****************************987788 PP TIGR02188 480 ..lyftGDgarrdkdGyiwilGRvDdvinvsGhrlgtaeiesalvsheavaeaavvgvpdeikgeaiva 546 +yf+GDgar+d+dGy+w++GRvDdv+nvsGhrlgt+e+esalvsh+avaeaavvg+pd++kge +va lcl|NCBI__GCF_000204075.1:WP_011318049.1 506 nyTYFAGDGARKDEDGYFWVMGRVDDVLNVSGHRLGTMEVESALVSHPAVAEAAVVGKPDDLKGEEVVA 574 989****************************************************************** PP TIGR02188 547 fvvlkegveedeeelekelkklvrkeigpiakpdkilvveelPktRsGkimRRllrkiaegeellgdvs 615 fv+l+ +++++++ l+k lk++v +eig+ia+p +i+++++lPktRsGkimRRllr++a+g+e+ gd+s lcl|NCBI__GCF_000204075.1:WP_011318049.1 575 FVTLEGTYQASDD-LSKVLKQHVVQEIGAIARPGEIRFTDALPKTRSGKIMRRLLRNLAAGQEVSGDTS 642 **********995.******************************************************* PP TIGR02188 616 tledpsvveelke 628 tled+sv+++l+e lcl|NCBI__GCF_000204075.1:WP_011318049.1 643 TLEDRSVLDKLRE 655 *********9986 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (629 nodes) Target sequences: 1 (657 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.02u 0.01s 00:00:00.03 Elapsed: 00:00:00.03 # Mc/sec: 12.55 // [ok]
This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory