Alignments for a candidate for frcA in Trichormus variabilis ATCC 29413

GapMind for catabolism of small carbon sources

Alignments for a candidate for frcA in Trichormus variabilis ATCC 29413

Align ABC-type sugar transport system, ATP-binding protein; EC 3.6.3.17 (characterized, see rationale)
to candidate WP_011318959.1 AVA_RS10980 sugar ABC transporter ATP-binding protein

Query= uniprot:A0A0C4Y5F6
         (540 letters)



>NCBI__GCF_000204075.1:WP_011318959.1
          Length = 517

 Score =  498 bits (1282), Expect = e-145
 Identities = 280/516 (54%), Positives = 361/516 (69%), Gaps = 10/516 (1%)

Query: 2   SDMSDTSTKAPLLALRNICKTFPGVRALRKVELTAYAGEVHALMGENGAGKSTLMKILSG 61
           +D S  +T  P+L ++ I K F GV AL+ V LT Y GEVHALMGENGAGKSTLMKIL+G
Sbjct: 7   TDFSPPATATPVLEMQGIAKRFHGVPALQGVNLTIYPGEVHALMGENGAGKSTLMKILAG 66

Query: 62  AYTADPGGECHIDGQRVQIDGPQSARDLGVAVIYQELSLAPNLSVAENIYLGRALQRRGL 121
           AY AD G E  I+G+ V+I  P +AR  G+ +IYQEL++APNL+V ENI++G  L R   
Sbjct: 67  AYIADEG-EIRINGKGVKITDPGTARQAGINLIYQELNVAPNLTVTENIFMGSELTRGQF 125

Query: 122 VARGDMVRACAPTLARLGADFSPAANVASLSIAQRQLVEIARAVHFEARILVMDEPTTPL 181
           + R  M       LA LGA F+P   V +L+IA++Q VEIARA+   +RILVMDEPT  L
Sbjct: 126 LDRKAMELEAQQVLASLGASFAPTDIVGTLAIAEQQQVEIARALKDNSRILVMDEPTAAL 185

Query: 182 STHETDRLFALIRQLRGEGMAILYISHRMAEIDELADRVTVLRDGCFVGTLDRAHLSQAA 241
           S  ET+RLF +IR+LR +G+AI+YISHRM EI  LADR++VLRDG ++G+L R  +S   
Sbjct: 186 SDRETERLFEVIRKLRNDGIAIIYISHRMEEIYALADRISVLRDGQYIGSLTREEISPQR 245

Query: 242 LVKMMVGRDLSGFYTKTHGQAVEREVMLSVRDVADGRRVKGCSFDLRAGEVLGLAGLVGA 301
           LV+MMVGR +  FY +   Q+    V+L VR+++DGR+V+  SF LRAGE+LGLAGLVGA
Sbjct: 246 LVQMMVGRSMQDFY-EHQRQSNPGPVVLEVRNISDGRKVQPASFQLRAGEILGLAGLVGA 304

Query: 302 GRTELARLVFGADARTRGEVRIANPAGSGGLVTLPAGGPRQAIDAGIAYLTEDRKLQGLF 361
           GRTE++RL+FGAD +  GEV +           L    P  AI  GI Y+ EDRK QGLF
Sbjct: 305 GRTEVSRLIFGADRKVSGEVFLNGK-------KLEIHSPSDAIAVGIGYVPEDRKDQGLF 357

Query: 362 LDQSVHENINLIVAARDALGLGRLNRTAARRRTTEAIDTLGIRVAHAQVNVGALSGGNQQ 421
           L+ S  +NI L    +DA  LG +N  +  +  T+A++   IR+A+ ++    LSGGNQQ
Sbjct: 358 LEMSSRKNIGLNRLKQDA-NLGIVNWGSVNKIATDAVENFHIRLANLEIRAVDLSGGNQQ 416

Query: 422 KVMLSRLLEIQPRVLILDEPTRGVDIGAKSEIYRLINALAQSGVAILMISSELPEVVGLC 481
           K++L+R L I PRVL+LDEPTRGVDIGAKSEIYR+I+ L+  GVAILM+SSELPE+VGL 
Sbjct: 417 KLLLARWLAINPRVLMLDEPTRGVDIGAKSEIYRIISDLSAQGVAILMVSSELPEIVGLS 476

Query: 482 DRVLVMREGTLAGEVRPAGSAAETQERIIALATGAA 517
           DRVLVMREG L GE+  +     TQE I+  ATGA+
Sbjct: 477 DRVLVMREGQLVGELDNSIGKEITQENIMHYATGAS 512


Lambda     K      H
   0.320    0.136    0.382 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 667
Number of extensions: 30
Number of successful extensions: 9
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 540
Length of database: 517
Length adjustment: 35
Effective length of query: 505
Effective length of database: 482
Effective search space:   243410
Effective search space used:   243410
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources