Align aconitate hydratase (EC 4.2.1.3) (characterized)
to candidate WP_011365887.1 GMET_RS09660 bifunctional aconitate hydratase 2/2-methylisocitrate dehydratase
Query= BRENDA::P36683 (865 letters) >NCBI__GCF_000012925.1:WP_011365887.1 Length = 847 Score = 1025 bits (2649), Expect = 0.0 Identities = 535/858 (62%), Positives = 640/858 (74%), Gaps = 18/858 (2%) Query: 1 MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEFLLDLLTNRVPPGVDEA 60 M+E Y H AER A+GI PL+ Q A L LL+NPPAG+EEFLL+LL RV PGVD A Sbjct: 1 MIEAYLAHEAERKAQGIPALPLNPEQTADLCNLLQNPPAGKEEFLLNLLKERVSPGVDPA 60 Query: 61 AYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHPLIDALDDAKLAPIAAKALS 120 A VKA FLA I KG KSPL++ AI +LGTM GGYN+ PL++AL DA+LA AA ALS Sbjct: 61 AKVKAAFLAEIVKGTKKSPLVSKVDAIRILGTMIGGYNVGPLVEALKDAELAEEAACALS 120 Query: 121 HTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRPALAEKLTVTVFKVTGETNTD 180 L++D F V E +K+ N AK+V++SWA+AEWF NRP + E + V VFKV GE NTD Sbjct: 121 RMTLVYDGFDQVVELSKS-NAAAKKVLESWANAEWFTNRPGVPETIKVKVFKVEGEINTD 179 Query: 181 DLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVGPIKQIEALQQKGFPLAYVGDVV 240 D SPA DAWSRPDIPLHALAM K P++ +K I + G +A+VGDVV Sbjct: 180 DFSPAGDAWSRPDIPLHALAMGKTRF----PNR------LKDIADWRAAGHQVAFVGDVV 229 Query: 241 GTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGGKIAPIFFNTMEDAGALPIEVDVSN 300 GTGSSRKSA NSVLW MG DIP VPNK+ G+ +GG IAPIFFNT +D+GALP++ DV+ Sbjct: 230 GTGSSRKSACNSVLWHMGQDIPAVPNKKTAGVIIGGVIAPIFFNTAQDSGALPLKADVTK 289 Query: 301 LNMGDVIDVYPYKGEVRNHETGELLATFELKTDVLIDEVRAGGRIPLIIGRGLTTKAREA 360 +N GDVI + KGE+ N + GE+++TF++ + L DE RAGGRIPLIIGR +T KAR+A Sbjct: 290 MNDGDVITINTAKGEISN-DKGEVISTFKISPNTLADEFRAGGRIPLIIGRAITEKARKA 348 Query: 361 LGLPHSDVFRQAKDVA-ESDRGFSLAQKMVGRACGVKGIRPGAYCEPKMTSVGSQDTTGP 419 LGL +DVF + + A ++ +G+SLAQKMVG+ACGV GI PG CEPKMT+VGSQDTTGP Sbjct: 349 LGLGDTDVFTKPVNPAPKAGQGYSLAQKMVGKACGVAGILPGTACEPKMTTVGSQDTTGP 408 Query: 420 MTRDELKDLACLGFSADLVMQSFCHTAAYPKPVDVNTHHTLPDFIMNRGGVSLRPGDGVI 479 MT DELK+LACL F + + MQSFCHTAAYPKP DV H LP FI+ RGGV L+PGDGVI Sbjct: 409 MTADELKELACLKFLSPMFMQSFCHTAAYPKPADVKMHKNLPGFIIERGGVPLKPGDGVI 468 Query: 480 HSWLNRMLLPDTVGTGGDSHTRFPIGISFPAGSGLVAFAAATGVMPLDMPESVLVRFKGK 539 HSWLNR+LLPDTVGTGGDSHTRFPIGISFPAGSGLVAFA A G MPLDMPESVLVRFKGK Sbjct: 469 HSWLNRLLLPDTVGTGGDSHTRFPIGISFPAGSGLVAFAGAMGFMPLDMPESVLVRFKGK 528 Query: 540 MQPGITLRDLVHAIPLYAIKQGLLTVEKKGKKNIFSGRILEIEGLPDLKVEQAFELTDAS 599 + PGITLRD V+AIP +AIKQG LTV KK K NIF+GRILE+EGLPDL VEQAFELTDA+ Sbjct: 529 LNPGITLRDAVNAIPYWAIKQGKLTVPKKNKINIFNGRILEMEGLPDLTVEQAFELTDAA 588 Query: 600 AERSAAGCTIKLNKEPIIEYLNSNIVLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLE 659 AERSAA I+L+KE + YL SN+ L+K MIA+GY D +TL+ RI + +WL NP+LLE Sbjct: 589 AERSAAAGCIQLSKESVATYLRSNVALMKKMIADGYQDAQTLQNRIDAVNEWLKNPQLLE 648 Query: 660 ADADA----EYAAVIDIDLADIKEPILCAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIG 715 AD +A YA VI+IDLA+I EPIL PNDPDD + LS V G I +VF+GSCMTNIG Sbjct: 649 ADKNAVETGAYADVIEIDLAEITEPILACPNDPDDVKLLSDVAGTPIQDVFLGSCMTNIG 708 Query: 716 HFRAAGKLLDAHKGQLPTRLWVAPPTRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGN 775 HFRAA ++ K R W+ PPTRMD QL +E Y+SV+ GARIEI GCSLCMGN Sbjct: 709 HFRAAAEIWRGLKFNPSVRTWICPPTRMDQKQLKDEAYFSVYSAFGARIEIAGCSLCMGN 768 Query: 776 QARVADGATVVSTSTRNFPNRLGTGANVFLASAELAAVAALIGKLPTPEEYQ-TYVAQVD 834 QARV DG + STSTRNF +R+G GA VFL SAEL AV A +GKLPT E+ Y +V+ Sbjct: 769 QARVPDGVNMFSTSTRNFDDRIGDGAKVFLGSAELGAVTATLGKLPTVAEFMAAYKEKVE 828 Query: 835 KTAVDTYRYLNFNQLSQY 852 Y+YL F+++ +Y Sbjct: 829 PKKDVVYKYLQFDEMPEY 846 Lambda K H 0.317 0.136 0.400 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 1903 Number of extensions: 80 Number of successful extensions: 6 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 865 Length of database: 847 Length adjustment: 42 Effective length of query: 823 Effective length of database: 805 Effective search space: 662515 Effective search space used: 662515 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 56 (26.2 bits)
This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory