Alignments for a candidate for pccB in Frankia alni ACN14A

GapMind for catabolism of small carbon sources

Alignments for a candidate for pccB in Frankia alni ACN14A

Align propionyl-CoA carboxylase α subunit (EC 6.4.1.3) (characterized)
to candidate WP_011604307.1 FRAAL_RS13795 acetyl-CoA carboxylase subunit beta

Query= metacyc::MONOMER-17283
         (535 letters)



>NCBI__GCF_000058485.1:WP_011604307.1
          Length = 560

 Score =  705 bits (1819), Expect = 0.0
 Identities = 350/532 (65%), Positives = 406/532 (76%)

Query: 4   IHSHIQPNSPDFQANFAYHQSLAADLRERLAQIRQGGGAEQRRRHEERGKLFVRDRIDTL 63
           + S +   SP  +     H +L A+LRERLA    GG    RRRH ERGKL  R+RI+ L
Sbjct: 29  LRSAVDAASPAARGYSETHTALVAELRERLAAAALGGPEPVRRRHVERGKLLARERIEVL 88

Query: 64  IDPDSSFLEIGALAAYNVYDEEVPAAGIVCGIGRVAGRPVMIIANDATVKGGTYFPLTVK 123
           +DP S FLE+  LAA  +YD + P AGIV GIGR+AGR  +++ANDATVKGGTY+PLTVK
Sbjct: 89  LDPGSPFLELSPLAADGMYDGDAPGAGIVTGIGRIAGRECVVVANDATVKGGTYYPLTVK 148

Query: 124 KHLRAQEIARENRLPCIYLVDSGGAYLPLQSEVFPDRDHFGRIFYNQAQMSAEGIPQIAC 183
           KHLRAQE+A  NRLPC+YLVDSGGA+LP+Q +VFPDR+HFGRIFYNQA MS  GI QIA 
Sbjct: 149 KHLRAQEVALHNRLPCVYLVDSGGAFLPMQDDVFPDREHFGRIFYNQATMSKAGIAQIAA 208

Query: 184 VMGSCTAGGAYVPAMSDEVVIVKGNGTIFLGGPPLVKAATGEEVTAEELGGADVHTRISG 243
           V+GS TAGGAYVPAMSDE VIV+  GTIFLGGPPLVKAATGE V AE+LGG  +H + SG
Sbjct: 209 VLGSSTAGGAYVPAMSDETVIVRNQGTIFLGGPPLVKAATGEVVAAEDLGGGLLHAKTSG 268

Query: 244 VADYFANDDREALAIVRDIVAHLGPRQRANWELRDPEPPRYDPREIYGILPRDFRQSYDV 303
           V D+ A+DD +AL  VR IVA+L PR    W +   E P  DP  +Y  +P D R  YDV
Sbjct: 269 VVDHLADDDADALQTVRRIVANLAPRAARPWPVAAVEEPAVDPAGLYAAVPTDSRTPYDV 328

Query: 304 REVIARIVDGSRLHEFKTRYGTTLVCGFAHIEGFPVGILANNGILFSESALKGAHFIELC 363
           REVIARIVDGSR  EFK  YG+TLV G A + G PVGI+ANNG+LF ESALKGAHFIELC
Sbjct: 329 REVIARIVDGSRFAEFKREYGSTLVTGTARLHGHPVGIVANNGVLFGESALKGAHFIELC 388

Query: 364 CARNIPLVFLQNITGFMVGKQYENGGIAKDGAKLVTAVSCANVPKFTVIIGGSFGAGNYG 423
             R IPLVFLQNI+GFMVG++YE GGIAK GAK+VTAV+CA VPKFTV+IGGSFGAGNY 
Sbjct: 389 DQRGIPLVFLQNISGFMVGREYEAGGIAKHGAKMVTAVACARVPKFTVVIGGSFGAGNYS 448

Query: 424 MCGRAYQPRQLWMWPNARISVMGGTQAANVLLTIRRDNLRARGQDMTPEEQERFMAPILA 483
           MCGRAY PR LWMWPNARISVMGG QAA VL T+RR+   ARGQD   E +E F API  
Sbjct: 449 MCGRAYSPRFLWMWPNARISVMGGEQAAAVLATVRREQYAARGQDWPAEAEEDFRAPIRE 508

Query: 484 KYEQEGHPYYASARLWDDGVIDPVETRRVLALGLAAAAEAPVQPTRFGVFRM 535
           +YE +G PYY++ARLWDDGVIDP +TR VL L L+ AA AP+ P  +G+FRM
Sbjct: 509 RYEVQGSPYYSTARLWDDGVIDPRDTRTVLGLALSVAANAPMDPPGYGIFRM 560


Lambda     K      H
   0.322    0.139    0.423 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1012
Number of extensions: 50
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 535
Length of database: 560
Length adjustment: 36
Effective length of query: 499
Effective length of database: 524
Effective search space:   261476
Effective search space used:   261476
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 53 (25.0 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources