Align actP-like component of L-lactate and L-malate uptake system (characterized)
to candidate WP_011612852.1 TERY_RS16355 cation acetate symporter
Query= reanno::PV4:5209923 (572 letters) >NCBI__GCF_000014265.1:WP_011612852.1 Length = 560 Score = 631 bits (1627), Expect = 0.0 Identities = 325/566 (57%), Positives = 415/566 (73%), Gaps = 26/566 (4%) Query: 2 DVQTLTYLIVGFTFALYIGIAIW-SRAGSTKEFYVAGGGVHPVMNGMATAADWMSAASFI 60 +V TL ++ V FT LYIG W SR +K F+VA V + NG AT ADWMSAASFI Sbjct: 4 EVWTLIFVAVSFTGYLYIG---WRSRVKDSKGFFVADQSVPAIANGAATGADWMSAASFI 60 Query: 61 SLAGIVSFVGYDGSVYLMGWTGGYVLLALCMAPYLRKFGKFTVPDFIGDRYYSQAARTVA 120 S+AG++SF+GYDGS+YLMGWTGGYVLLAL +APYLRKFGK+TVPDF+GDRYYS AR VA Sbjct: 61 SMAGLISFLGYDGSIYLMGWTGGYVLLALLLAPYLRKFGKYTVPDFVGDRYYSNVARIVA 120 Query: 121 VVCAIFICFTYIAGQMRGVGVVFSRFLEVEVDTGVYIGMAVVFFYAVLGGMKGITYTQVA 180 V+ AIF+ TY+AGQMRGVG+VFSRFL+V++ TGV+IGM +V F+A+LGGMKGIT+TQVA Sbjct: 121 VIAAIFVSMTYVAGQMRGVGIVFSRFLQVDIGTGVFIGMIIVAFFAILGGMKGITWTQVA 180 Query: 181 QYCVLIFAFMVPAIFISVMMTGHILPQLGFGAELVDAAGNNTGVYLLEKLDGLSAQLGFS 240 QY VLI A+++PAI I+ ++TG +PQ F T ++EKL+ + LGF+ Sbjct: 181 QYTVLIIAYLIPAIAIATVITGIPIPQFAF-----------TFSDIVEKLNQVQVDLGFA 229 Query: 241 QYTE--GSKGMIDVFFITGALMFGTAGLPHVIVRFFTVPKVKDARVSAGWALVFIAIMYT 298 +YT +K M+DV FIT ALM GTAGLPHVIVRF+TVP V+ AR SAGWAL+FIAI+YT Sbjct: 230 EYTAAFSNKTMLDVLFITIALMVGTAGLPHVIVRFYTVPDVRAARYSAGWALLFIAILYT 289 Query: 299 TIPALAAFSRVNMIETINGPESTGVAYETAPDWIKNWEKTGLIKWDDKNNDGKIYYAKG- 357 T PALA F+R N+I++++ V +W WE TGL+++ DKNNDG+ Sbjct: 290 TAPALATFARYNLIDSLH---DKTVEEVQQLNWATKWENTGLLEFTDKNNDGRFQLTPDK 346 Query: 358 ETNEMKIDRDIMVLATPEIANLPAWVIALVAAGGLAAALSTSAGLLLVISTSVSHDLLKK 417 ETNE+KIDRDI+VL+TPE+A L WVIALVAAGGLAAALST++GLLLVIS+S++HD+ + Sbjct: 347 ETNEIKIDRDIIVLSTPEVAKLAPWVIALVAAGGLAAALSTASGLLLVISSSIAHDVYYR 406 Query: 418 NFMPDISDKQELLYARIAAALGIVMAGYFGINPPGFVAAVVAIAFGLAASSLFPAIIMGI 477 P + + L+ R+ I +AGYFG+NPPGFVA VVA AFGLAA+S FP II+G+ Sbjct: 407 MIDPGAPESRRLIVGRMMVGFSIAIAGYFGVNPPGFVAQVVAFAFGLAAASFFPVIILGV 466 Query: 478 FSRTMNKEGAIAGMVIGLLFSASYIIYFKFVNPGDNNASNWLFGISPEGIGMLGMIINFA 537 F + N+EGAIAGMV+GLLF+ YI+ KF W FG+S EGIG +GM +NF Sbjct: 467 FDKRTNREGAIAGMVVGLLFTTIYIVGVKFA-----GMPTWFFGVSAEGIGTVGMALNFI 521 Query: 538 VAFIVSKVTAAVPQNVVDMVESIRFP 563 V FIVS++T P + +MVE++R P Sbjct: 522 VTFIVSRMTPPPPLEIQEMVENLRSP 547 Lambda K H 0.326 0.140 0.423 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 1072 Number of extensions: 49 Number of successful extensions: 9 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 572 Length of database: 560 Length adjustment: 36 Effective length of query: 536 Effective length of database: 524 Effective search space: 280864 Effective search space used: 280864 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 53 (25.0 bits)
This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory