Align PTS system fructose-specific EIIA component; EIIA-Fru; Fructose-specific phosphotransferase enzyme IIA component (characterized)
to candidate WP_011650215.1 RL_RS02215 PTS IIA-like nitrogen-regulatory protein PtsN
Query= SwissProt::D4GYE4 (154 letters) >NCBI__GCF_000009265.1:WP_011650215.1 Length = 154 Score = 85.1 bits (209), Expect = 4e-22 Identities = 47/125 (37%), Positives = 67/125 (53%), Gaps = 2/125 (1%) Query: 29 EFLLDLAVDAGRVD--DRDAALDALLEREGEATTGVGFGIGIPHAKTDAVSKPTVAFARS 86 + L +LA A R+ D +L+RE +TGVG GI IPH K + FAR Sbjct: 23 QLLQELAARAARITGLSEREIFDVILQRERLGSTGVGNGIAIPHGKLGNIHSIVGIFARL 82 Query: 87 AEGIDFDAMDDKPAKLLFMILVPAAGGEDHLQILSALSRSLMHEDVREKLLEAESKQTVQ 146 + +DF+A+DD+P L+F++L P G DHL+ LS ++R L D+ KL ES + Sbjct: 83 EQPVDFEALDDQPVDLVFLLLAPEGAGADHLKALSRIARVLRDHDLVAKLRATESASAIY 142 Query: 147 DVLAE 151 L E Sbjct: 143 AFLNE 147 Lambda K H 0.315 0.134 0.359 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 56 Number of extensions: 3 Number of successful extensions: 1 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 154 Length of database: 154 Length adjustment: 17 Effective length of query: 137 Effective length of database: 137 Effective search space: 18769 Effective search space used: 18769 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 43 (21.2 bits)
This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory