Align gluconate 5-dehydrogenase (EC 1.1.1.69); D-sorbitol dehydrogenase (acceptor) (subunit 1/2) (EC 1.1.99.21) (characterized)
to candidate WP_011651065.1 RL_RS07055 glucose/quinate/shikimate family membrane-bound PQQ-dependent dehydrogenase
Query= BRENDA::Q70JN9 (743 letters) >NCBI__GCF_000009265.1:WP_011651065.1 Length = 779 Score = 483 bits (1243), Expect = e-140 Identities = 266/660 (40%), Positives = 362/660 (54%), Gaps = 24/660 (3%) Query: 83 DIGSMDPNDVPQMAPQQSASPASGDWAAYGHDDSQMRYSPLSEITPQNADQLKVAFVYHT 142 D+ P D P S G+W YG RYSPL +IT +N LK A+ Y T Sbjct: 141 DLAGELPKDTVAANPAFGGSVPDGEWHQYGRTPFGQRYSPLDQITAENVSTLKEAWRYQT 200 Query: 143 GSYPRPGQTNKWAAETTPIKVGDGLYMCSAQNDIMKIDPATGKEIWRHNINEKYEAIPYT 202 G RP + + TP+KV D LY+C+ N + +D TGKE W+++ N Sbjct: 201 GDVKRPDDVGETTYQVTPLKVKDTLYLCTPHNWAIALDAKTGKEKWKYDANSGMNPDRQH 260 Query: 203 AACKGVTYFTSSQVPEGQPCHNRILEGTLDMRLIAVDAATGNLCEGFGNGGQVNLMQGLG 262 C+GVTY+ V GQPC R+ T D RLIA+DAA G +C F + G ++L G+ Sbjct: 261 QTCRGVTYYADPAVAAGQPCAERVYLPTSDARLIALDAADGKVCTSFADQGVLHLETGMR 320 Query: 263 ESVPGFVSMTTPPPVVNGVVVVNHEVLDGQRRWAPSGVIRGYDAESGKFLWAWDVNRPND 322 + G+ T+PP V G ++V V D SGVIR +D +G +W WD P+ Sbjct: 321 YNPAGYYYSTSPPVAVAGKIIVGGAVNDNYSTEEQSGVIRAFDINTGALVWNWDSGNPDL 380 Query: 323 HSQPTGNNHYSRGTPNSWAAMTGDNALGLVYVPTGNSASDYYSALRSPEENKVSSAVVAL 382 + T Y+ +PNSW+ + D ALG+VY+P GN D RS K SS++VAL Sbjct: 381 TTPITEGQTYTTNSPNSWSVFSVDEALGMVYIPLGNQVPDQIGINRSDNVEKFSSSIVAL 440 Query: 383 DVKTGSPRWVFQTVHKDVWDYDIGSQATLMDMPGQDGQPVPALIMPTKRGQTFVLDRRDG 442 D+ TG RWV QTVH D+WD D+ +Q L+D+ QDG VPAL+ PTK+G +VLDRR G Sbjct: 441 DIATGQLRWVRQTVHHDLWDMDVPAQPALIDLTKQDGTVVPALVGPTKQGDIYVLDRRSG 500 Query: 443 KPILPVEERPAPSPGVIPGDPRSPTQPWSTGMPALRVPDLKETDMWGMSPIDQLFCRIKF 502 +PI+PV+E PAP G + GD SPTQP S L+E DMWG+S DQ+ CRI F Sbjct: 501 EPIIPVKEIPAPG-GAVSGDHTSPTQPISD--LTFSPEPLQEKDMWGVSLFDQMACRIDF 557 Query: 503 RRANYTGEFTPPSVDKPWIEYPGYNGGSDWGSVSYDPQSGILIANWNITPMYDQLVTRKK 562 R +Y G +TPPS+ K I YPG G +WGSV+ DP+ I+ P Y +R Sbjct: 558 HRYHYEGRYTPPSL-KGTIVYPGNFGTFNWGSVAVDPERQIMFG----MPTYLAFTSR-- 610 Query: 563 ADELGLMPIDD--PNYKPGGGGAEGNGAMDGTPYGIVVTPFWDQYTGMMCNRPPYGMITA 620 L+P D P + G +G DG PYG+ + PF + C PP+G + Sbjct: 611 -----LVPAADIPPRGQDEKGSEQGLNRNDGAPYGVFMGPFLG-LLQIPCQAPPWGYVAG 664 Query: 621 IDMKHGQKVLWQHPLGTARANGPWGLPTGLPWEIGTPNNGGSVVTAGGVVFIAAATDNQI 680 +D++ G K+ + H GT P LP LP+++G P GG ++T GGV F+ AA DN + Sbjct: 665 VDLRTG-KIAYMHKNGTVHDMTP--LP--LPFKVGVPGIGGPMLTKGGVAFLGAAVDNYL 719 Query: 681 RAIDEHTGKVVWSAVLPGGGQANPMTYEANGH-QYVAIMAGGHHFMMTPVSDQLVVYALP 739 RA D +G+ +W LP GGQA PMTY + + QYV ++AGGH + T D ++ Y LP Sbjct: 720 RAYDVTSGRELWQGRLPAGGQATPMTYMTDDNKQYVVMVAGGHGSVGTTPGDYVIAYTLP 779 Lambda K H 0.315 0.134 0.428 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 2142 Number of extensions: 149 Number of successful extensions: 13 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 743 Length of database: 779 Length adjustment: 40 Effective length of query: 703 Effective length of database: 739 Effective search space: 519517 Effective search space used: 519517 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 55 (25.8 bits)
This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory