Align 2-methylbutanoyl-CoA dehydrogenase / butanoyl-CoA dehydrogenase / isobutyryl-CoA dehydrogenase (EC 1.3.8.1; EC 1.3.8.5) (characterized)
to candidate WP_011654331.1 RL_RS28910 acyl-CoA dehydrogenase
Query= reanno::pseudo3_N2E3:AO353_25680 (375 letters) >NCBI__GCF_000009265.1:WP_011654331.1 Length = 375 Score = 554 bits (1428), Expect = e-162 Identities = 273/375 (72%), Positives = 324/375 (86%) Query: 1 MLPTDEQLQISDAARQFAQERLKPFAAEWDREHRFPKEAIGEMAELGFFGMLVPEQWGGC 60 M+ +D Q QISD AR FA++RL P AA+ DREH FP+E + EM ELG GMLVPE +GG Sbjct: 1 MILSDLQQQISDLARDFARDRLAPGAAKRDREHLFPREELKEMGELGLLGMLVPEAYGGS 60 Query: 61 DTGYLAYAMALEEIAAGDGACSTIMSVHNSVGCVPILKFGNDDQKERFLKPLASGAMLGA 120 DTG +AYA ALEEIAAGDG CSTIMSVH+SVGCVPILKFG ++Q++RFL LASG +G Sbjct: 61 DTGVVAYAAALEEIAAGDGPCSTIMSVHSSVGCVPILKFGTEEQRQRFLPKLASGEWIGG 120 Query: 121 FALTEPQAGSDASSLKTRARLNGDHYVLNGCKQFITSGQNAGVVIVFAVTDPSAGKRGIS 180 FALTEPQAGSDAS+LKTRAR +GD+YVL+G KQFITSG+N V+IVFAVTDP GK+GI+ Sbjct: 121 FALTEPQAGSDASNLKTRARRDGDYYVLDGSKQFITSGKNGDVIIVFAVTDPDVGKKGIT 180 Query: 181 AFIVPTDSPGYKVARVEDKLGQHASDTCQILFEDVQVPVANRLGEEGEGYKIALANLEGG 240 AFIVPTD+PGY+V RVE+KLG H+SDTCQI F +++P RLG EGEGY+IALANLEGG Sbjct: 181 AFIVPTDTPGYEVIRVEEKLGLHSSDTCQIAFNSMRIPAELRLGAEGEGYRIALANLEGG 240 Query: 241 RVGIASQSVGMARAAFEAARDYARERESFGKPIIEHQAVAFRLADMATQIAVARQMVHYA 300 R+GIA+Q+VGMARAAFEAARDYARER +FGKPI+EHQAVAFRLADMA +I ARQ+V +A Sbjct: 241 RIGIAAQAVGMARAAFEAARDYARERIAFGKPIVEHQAVAFRLADMAVRIEAARQLVFHA 300 Query: 301 AALRDSGKPALVEASMAKLFASEMAEKVCSTALQTLGGYGYLSDFPLERIYRDVRVCQIY 360 A+LR++G P L EASMAKLFASEMAE+VCS A+Q GGYGY++D+P+ERIYRDVR+CQIY Sbjct: 301 ASLREAGLPCLSEASMAKLFASEMAERVCSDAIQIHGGYGYMADYPVERIYRDVRICQIY 360 Query: 361 EGTSDIQRMVISRNL 375 EGTSD+QRMVI+RNL Sbjct: 361 EGTSDVQRMVIARNL 375 Lambda K H 0.319 0.134 0.389 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 476 Number of extensions: 15 Number of successful extensions: 2 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 375 Length of database: 375 Length adjustment: 30 Effective length of query: 345 Effective length of database: 345 Effective search space: 119025 Effective search space used: 119025 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 50 (23.9 bits)
This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory