Align methylmalonyl-CoA mutase (subunit 2/2) (EC 5.4.99.2) (characterized)
to candidate WP_011734468.1 PPRO_RS02565 methylmalonyl-CoA mutase
Query= BRENDA::O74009 (563 letters) >NCBI__GCF_000015045.1:WP_011734468.1 Length = 551 Score = 710 bits (1832), Expect = 0.0 Identities = 358/554 (64%), Positives = 439/554 (79%), Gaps = 9/554 (1%) Query: 12 IKEEEKRWEETTVKKFLEKAPERKEKFMTDDGFEIKRIYTPADLGEDWNYM---EKLGFP 68 I E++K+W +T V KFL ++PER EKF+T EI+R++TP D++Y EKLGFP Sbjct: 3 IAEKKKQWTDTKVAKFLTRSPERYEKFLTTSNLEIERVFTP-----DFDYPGYDEKLGFP 57 Query: 69 GEYPFTRGVYATMYRGRIWTMRQYAGYATAEESNKRYKYLLSQGQTGLSVAFDLPTQLGY 128 G YP+TRGV TMYRGR WTMRQYAG+ A+ESN+RYKYLL GQTGLS+AFDLPTQ+GY Sbjct: 58 GSYPYTRGVQPTMYRGRFWTMRQYAGFGNAKESNERYKYLLGAGQTGLSIAFDLPTQMGY 117 Query: 129 DSDHPLAEGEVGKVGVAIDSLWDMRILFDGIPLDKVSTSMTINSTAANLLAMYILVAEEQ 188 DSD +++GEVGKVGVAIDSL D+ +LFDGIPLDKVSTSMT+NSTAA LL MYI VAE+Q Sbjct: 118 DSDAAMSQGEVGKVGVAIDSLADVEVLFDGIPLDKVSTSMTVNSTAAILLCMYIAVAEKQ 177 Query: 189 GVSQEKLRGTVQNDILKEYIARGTYIFPPQPSMRLTTDIIMYCAENVPKWNPISISGYHI 248 GVS +K+ GT+QNDILKEY+ARGTYI+PP+ SMRL TDI YC +NVPKWN ISISGYHI Sbjct: 178 GVSADKISGTIQNDILKEYMARGTYIYPPKESMRLITDIFAYCKDNVPKWNTISISGYHI 237 Query: 249 REAGANAVQEVAFTLADGIEYVKAVIERGMDVDKFAPRLSFFFAAHNNFLEEIAKFRAAR 308 REAG++AVQEVAFTLADGI YV A I+ G+DVD FAPRL+FFF AHNN LEE+AKFRAAR Sbjct: 238 REAGSSAVQEVAFTLADGIAYVDAAIKAGLDVDDFAPRLAFFFNAHNNLLEEVAKFRAAR 297 Query: 309 RLWAYIMKEWFNAKNPRSMMLRFHTQTAGSTLTAQQPENNIVRVAIQALAAVLGGTQSLH 368 R+WA IMKE F AK +S MLRFHTQTAG TLTAQQP+NNI+RV IQ LAAVLGGTQSLH Sbjct: 298 RIWAKIMKERFGAKLEKSQMLRFHTQTAGCTLTAQQPDNNIMRVTIQTLAAVLGGTQSLH 357 Query: 369 TNSYDEALSLPTEKSVRIALRTQQIIAYESGVVDTVDPLGGAYYIEWLTDHIYEEALKYI 428 TNS DEAL+LPTE SVRIALRTQQ++AYESGV D++DPL G++++E LTD I + A +YI Sbjct: 358 TNSRDEALALPTEDSVRIALRTQQVVAYESGVADSIDPLAGSFFVESLTDQIEKAAWEYI 417 Query: 429 EKIQKMGGMMRAIERGYVQKEIAEAAYKYQKEIEEGKRIIVGVNAFVTDEPIEVEILKVD 488 EKI K+GG + AI G+ QKEI ++AY YQK IE+ + IIVGVN F EP +LKV Sbjct: 418 EKIDKLGGAVEAISNGFQQKEIQDSAYAYQKAIEKNELIIVGVNKFTISEPPPTGLLKVT 477 Query: 489 PSIREKQIERLKKLRSERDNKKVQEALDKLRNAAEKEDENLMPYIIEAHRHLATLQEVTD 548 + Q + L +++++RD V+ AL KL AA K +NL+P I+EA + ATL E+ D Sbjct: 478 GEVETNQKKALAEVKAKRDGAAVKAALAKLEVAA-KGTDNLIPLILEAVKVYATLGEIAD 536 Query: 549 VLREIWGEYRAPLI 562 V R ++G+++ ++ Sbjct: 537 VFRGVFGKHQETVV 550 Lambda K H 0.318 0.134 0.388 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 875 Number of extensions: 32 Number of successful extensions: 3 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 563 Length of database: 551 Length adjustment: 36 Effective length of query: 527 Effective length of database: 515 Effective search space: 271405 Effective search space used: 271405 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 53 (25.0 bits)
This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory