Alignments for a candidate for pccA in Pelobacter propionicus DSM 2379

GapMind for catabolism of small carbon sources

Alignments for a candidate for pccA in Pelobacter propionicus DSM 2379

Align Propionyl-CoA carboxylase biotin-containing subunit (EC 6.4.1.3) (characterized)
to candidate WP_011735249.1 PPRO_RS06630 pyruvate carboxylase

Query= reanno::PS:Dsui_0516
         (663 letters)



>NCBI__GCF_000015045.1:WP_011735249.1
          Length = 1148

 Score =  388 bits (996), Expect = e-112
 Identities = 209/453 (46%), Positives = 293/453 (64%), Gaps = 11/453 (2%)

Query: 2   FKKILIANRGEIACRVIKTARKMGIKTVAVYSEADKDALFVEMADEAVCIGPA-ASKESY 60
           FKKI+ ANRGEI+ R+ +   ++GI+ VA+YSE DK +L    ADEA  IG   A  E+Y
Sbjct: 6   FKKIMAANRGEISVRIFRACAELGIRMVAIYSEEDKLSLHRYKADEAYLIGKGKAPVEAY 65

Query: 61  LVADKIIAACKQTGAEAVHPGYGFLSENAEFSRRLEEEGIKFIGPKHYSIAKMGDKIESK 120
           L  D+IIA   + G EA+HPGYGFLSENAEF+ + E  GI FIGP       +GDK+  +
Sbjct: 66  LGIDEIIALALKVGVEAIHPGYGFLSENAEFAEKCEANGIVFIGPTGEMQRALGDKVAGR 125

Query: 121 KLAIEAKVNTIPGYNDAIDGPDAAVEIAKKIGYPVMIKASAGGGGKGLRVAYNDAEAHEG 180
           K+A  A VN +PG  D I+  + A++ AK+ GYP+++KA+AGGGG+G+RVA+N  E  EG
Sbjct: 126 KMATAAGVNVVPGTEDPIEREEDALKFAKQYGYPIIVKAAAGGGGRGMRVAHNQKELLEG 185

Query: 181 FSSCVNEARNSFGDDRVFIEKYVLEPRHIEIQVLGDSHGNYVYLNERDCSIQRRHQKVIE 240
             +  +EA+ +FG+  VF+E+Y+  P+HIE+QVLGD++GN V+  ERDCS+QRRHQKV+E
Sbjct: 186 LVAAGSEAKAAFGNAAVFLERYLENPKHIEVQVLGDNYGNLVHFFERDCSVQRRHQKVVE 245

Query: 241 EAPSPFVDPEMRKAMGEQAVALARAVNYESAGTVEFVVSGATKEFYFLEMNTRLQVEHPV 300
            APS  + PE+R+ + + A+ +AR VNY +AGTVEF+V    + +YF+EMN R+QVEH V
Sbjct: 246 FAPSLCITPELREELCDAALKIARQVNYRNAGTVEFLVDQQGR-YYFMEMNPRIQVEHTV 304

Query: 301 TELITGLDLVEQMIRVAYGEKLP------LTQADVQINGWAMECRINAEDPFRGFLPSTG 354
           TE+ITG +LV+  I VA G  L        +Q  + + G+A++CR+  EDP   F P  G
Sbjct: 305 TEMITGRNLVQNQILVAGGYALSDPEINIPSQGAINMRGYAIQCRVTTEDPANNFAPDFG 364

Query: 355 RLVKFQPPAEVDGQVRVDTG-VYDGGEISMYYDSMIAKLIVHGASREQAIARMRDALNGF 413
            L  ++  A     VR+D G    G  I+ +YDS++ K+   G +  +A   M  AL  F
Sbjct: 365 MLTTYRSAAGCG--VRLDAGNATTGARITPHYDSLLVKVGTWGLTFPEAAHIMDRALREF 422

Query: 414 VIRGISSNIPFQAALMQHARFQSGIFDTGFIAK 446
            IRG+ +NI F   ++ H  F  G  DT FI K
Sbjct: 423 RIRGVKTNIAFLENVVTHPVFLEGKCDTSFIDK 455



 Score = 56.2 bits (134), Expect = 8e-12
 Identities = 30/74 (40%), Positives = 46/74 (62%)

Query: 587  EKAAPDLSKFLLSPMPGLLREVSVAVGQEVKAGEKLAVIEAMKMENILKAEQDCKVKKIS 646
            +KA  D    + +PMPG + +V+V  G EVKAG+ L V EAMKME  +KA+ D +V ++ 
Sbjct: 1072 DKADRDNPSHIGAPMPGKVFKVNVKPGFEVKAGDVLMVTEAMKMETNIKAKVDGRVMEVK 1131

Query: 647  VTAGSSLSVDEIII 660
               G  +  D+++I
Sbjct: 1132 FKEGDKVEKDDLLI 1145


Lambda     K      H
   0.319    0.135    0.391 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1698
Number of extensions: 81
Number of successful extensions: 6
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 2
Number of HSP's successfully gapped: 2
Length of query: 663
Length of database: 1148
Length adjustment: 42
Effective length of query: 621
Effective length of database: 1106
Effective search space:   686826
Effective search space used:   686826
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 56 (26.2 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources